Details
| Original language | English |
|---|---|
| Pages (from-to) | 273-276 |
| Number of pages | 4 |
| Journal | IFAC Proceedings Volumes (IFAC-PapersOnline) |
| Volume | 40 |
| Issue number | 4 |
| Publication status | Published - 2007 |
| Event | 10th IFAC Symposium on Computer Applications in Biotechnology, 2007 - Cancun, Mexico Duration: 4 Jun 2007 → 6 Jun 2007 |
Abstract
We have developed a new probe that measures the oxygen uptake rate (OUR) of mammalian cells, an important indicator for the metabolic state, inside the reactor (in-situ) and in real-time without the need for sampling or complicated analytics. The probe isolates a known volume of cell culture from the bulk inside the bioreactor, monitors the oxygen consumption over time and releases it again. The sample is mixed during the measurement with a new agitation system to keep the cells in suspension and prevent oxygen concentration gradients. The OUR measurement system also doubles as a standard DO probe for process monitoring when it is not performing OUR measurements. This new probe was successfully tested in BHK perfusion cell cultures.
Keywords
- Automatic process control, Biomedical control, Biotechnology, Fermentation processes, Sensor integration, Sensor systems
ASJC Scopus subject areas
- Engineering(all)
- Control and Systems Engineering
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In: IFAC Proceedings Volumes (IFAC-PapersOnline), Vol. 40, No. 4, 2007, p. 273-276.
Research output: Contribution to journal › Conference article › Research › peer review
}
TY - JOUR
T1 - Development of a new probe for in-situ oxygen uptake rate (OUR) measurement in mammalian cell culture processes
AU - Joeris, K.
AU - Behr, L.
AU - Burnett, M.
AU - Scheper, T.
AU - Konstantinov, K.
PY - 2007
Y1 - 2007
N2 - We have developed a new probe that measures the oxygen uptake rate (OUR) of mammalian cells, an important indicator for the metabolic state, inside the reactor (in-situ) and in real-time without the need for sampling or complicated analytics. The probe isolates a known volume of cell culture from the bulk inside the bioreactor, monitors the oxygen consumption over time and releases it again. The sample is mixed during the measurement with a new agitation system to keep the cells in suspension and prevent oxygen concentration gradients. The OUR measurement system also doubles as a standard DO probe for process monitoring when it is not performing OUR measurements. This new probe was successfully tested in BHK perfusion cell cultures.
AB - We have developed a new probe that measures the oxygen uptake rate (OUR) of mammalian cells, an important indicator for the metabolic state, inside the reactor (in-situ) and in real-time without the need for sampling or complicated analytics. The probe isolates a known volume of cell culture from the bulk inside the bioreactor, monitors the oxygen consumption over time and releases it again. The sample is mixed during the measurement with a new agitation system to keep the cells in suspension and prevent oxygen concentration gradients. The OUR measurement system also doubles as a standard DO probe for process monitoring when it is not performing OUR measurements. This new probe was successfully tested in BHK perfusion cell cultures.
KW - Automatic process control
KW - Biomedical control
KW - Biotechnology
KW - Fermentation processes
KW - Sensor integration
KW - Sensor systems
UR - http://www.scopus.com/inward/record.url?scp=84944315856&partnerID=8YFLogxK
U2 - 10.3182/20070604-3-mx-2914.00047
DO - 10.3182/20070604-3-mx-2914.00047
M3 - Conference article
AN - SCOPUS:84944315856
VL - 40
SP - 273
EP - 276
JO - IFAC Proceedings Volumes (IFAC-PapersOnline)
JF - IFAC Proceedings Volumes (IFAC-PapersOnline)
SN - 1474-6670
IS - 4
T2 - 10th IFAC Symposium on Computer Applications in Biotechnology, 2007
Y2 - 4 June 2007 through 6 June 2007
ER -