Details
| Original language | English |
|---|---|
| Pages (from-to) | 497-507 |
| Number of pages | 11 |
| Journal | PLANT BREEDING |
| Volume | 140 |
| Issue number | 3 |
| Early online date | 24 May 2021 |
| Publication status | Published - 12 Jun 2021 |
Abstract
Poinsettia is an economically important ornamental potted plant in which certain bract colour variants are often obtained by mutation breeding. Previously, in poinsettia, we identified Bract1, a GST gene involved in the sequestration and transport of anthocyanins to the vacuole. This gene carries a short, highly mutable 4-bp repeat in its coding region. Loss of one repeat unit leads to a loss of function for Bract1, and in homozygous mutants, anthocyanin-based coloration is absent, resulting in white or cream-coloured bracts. Although mutation induction through ionizing radiation leads to a high frequency of mutations in Bract1, mutants are difficult to obtain from homozygous red genotypes. In this study, we used Bract1-specific amplicon sequencing as a tool to identify mutations in pools of tissues, which enabled the detection of mutations in dilutions of up to one mutant in 50 nonmutated samples. This approach enabled efficient screening of recalcitrant homozygous genotypes for mutated alleles and the reduction of the mutation load in the application of ionizing radiation in mutation breeding programmes.
Keywords
- amplicon-seq, Bract1, heterozygous mutations, irradiation mutagenesis, mutation breeding, poinsettia breeding
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: PLANT BREEDING, Vol. 140, No. 3, 12.06.2021, p. 497-507.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of a multiplex amplicon-sequencing assay to detect low-frequency mutations in poinsettia (Euphorbia pulcherrima) breeding programmes
AU - Vilperte, Vinicius
AU - Boehm, Robert
AU - Debener, Thomas
N1 - Funding Information: We would like to thank Guido von Tubeuf for providing the poinsettia varieties. This project has received funding from the European Union's Horizon 2020 Framework Programme (research and innovation programme) under the Marie Skłodowska Curie Grant Agreement No. 675657 Flower Power. This funding body had no role in the design of the study, in the collection, analysis or interpretation of data, or in writing the manuscript. The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover.
PY - 2021/6/12
Y1 - 2021/6/12
N2 - Poinsettia is an economically important ornamental potted plant in which certain bract colour variants are often obtained by mutation breeding. Previously, in poinsettia, we identified Bract1, a GST gene involved in the sequestration and transport of anthocyanins to the vacuole. This gene carries a short, highly mutable 4-bp repeat in its coding region. Loss of one repeat unit leads to a loss of function for Bract1, and in homozygous mutants, anthocyanin-based coloration is absent, resulting in white or cream-coloured bracts. Although mutation induction through ionizing radiation leads to a high frequency of mutations in Bract1, mutants are difficult to obtain from homozygous red genotypes. In this study, we used Bract1-specific amplicon sequencing as a tool to identify mutations in pools of tissues, which enabled the detection of mutations in dilutions of up to one mutant in 50 nonmutated samples. This approach enabled efficient screening of recalcitrant homozygous genotypes for mutated alleles and the reduction of the mutation load in the application of ionizing radiation in mutation breeding programmes.
AB - Poinsettia is an economically important ornamental potted plant in which certain bract colour variants are often obtained by mutation breeding. Previously, in poinsettia, we identified Bract1, a GST gene involved in the sequestration and transport of anthocyanins to the vacuole. This gene carries a short, highly mutable 4-bp repeat in its coding region. Loss of one repeat unit leads to a loss of function for Bract1, and in homozygous mutants, anthocyanin-based coloration is absent, resulting in white or cream-coloured bracts. Although mutation induction through ionizing radiation leads to a high frequency of mutations in Bract1, mutants are difficult to obtain from homozygous red genotypes. In this study, we used Bract1-specific amplicon sequencing as a tool to identify mutations in pools of tissues, which enabled the detection of mutations in dilutions of up to one mutant in 50 nonmutated samples. This approach enabled efficient screening of recalcitrant homozygous genotypes for mutated alleles and the reduction of the mutation load in the application of ionizing radiation in mutation breeding programmes.
KW - amplicon-seq
KW - Bract1
KW - heterozygous mutations
KW - irradiation mutagenesis
KW - mutation breeding
KW - poinsettia breeding
UR - http://www.scopus.com/inward/record.url?scp=85106321038&partnerID=8YFLogxK
U2 - 10.1111/pbr.12925
DO - 10.1111/pbr.12925
M3 - Article
AN - SCOPUS:85106321038
VL - 140
SP - 497
EP - 507
JO - PLANT BREEDING
JF - PLANT BREEDING
SN - 0179-9541
IS - 3
ER -