Details
| Original language | English |
|---|---|
| Pages (from-to) | 35-43 |
| Number of pages | 9 |
| Journal | Journal of virological methods |
| Volume | 243 |
| Publication status | Published - May 2017 |
Abstract
A nucleic acid based test for the detection of the economically important plant virus Cucumber mosaic virus (CMV) based on the Luminex xTAG technology was developed. This technology has the advantage of allowing the simultaneous detection of various targets. Applying this method, we prove the presence of CMV in general and differentiate between its two subgroups I and II for which significant differences concerning severity of symptoms and virulence have been reported. For the development of the test procedure the coat protein gene sequences of 29 CMV isolates were cloned, sequenced and classified into subgroups. Sequences from GenBank were used to design primers. Additionally, a subgroup specific ELISA was conducted for comparison. This work is part of a project which aims to develop a test for the simultaneous detection of various plant pathogens (viral, bacterial and fungal) in plant material.
Keywords
- Cucumber mosaic virus, ELISA, Luminex xTAG, Molecular diagnostics
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Virology
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In: Journal of virological methods, Vol. 243, 05.2017, p. 35-43.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of a molecular assay for the detection of Cucumber mosaic virus and the discrimination of its subgroups I and II
AU - Bald-Blume, N.
AU - Bergervoet, J. H.W.
AU - Maiss, E.
N1 - Publisher Copyright: © 2017 Elsevier B.V. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/5
Y1 - 2017/5
N2 - A nucleic acid based test for the detection of the economically important plant virus Cucumber mosaic virus (CMV) based on the Luminex xTAG technology was developed. This technology has the advantage of allowing the simultaneous detection of various targets. Applying this method, we prove the presence of CMV in general and differentiate between its two subgroups I and II for which significant differences concerning severity of symptoms and virulence have been reported. For the development of the test procedure the coat protein gene sequences of 29 CMV isolates were cloned, sequenced and classified into subgroups. Sequences from GenBank were used to design primers. Additionally, a subgroup specific ELISA was conducted for comparison. This work is part of a project which aims to develop a test for the simultaneous detection of various plant pathogens (viral, bacterial and fungal) in plant material.
AB - A nucleic acid based test for the detection of the economically important plant virus Cucumber mosaic virus (CMV) based on the Luminex xTAG technology was developed. This technology has the advantage of allowing the simultaneous detection of various targets. Applying this method, we prove the presence of CMV in general and differentiate between its two subgroups I and II for which significant differences concerning severity of symptoms and virulence have been reported. For the development of the test procedure the coat protein gene sequences of 29 CMV isolates were cloned, sequenced and classified into subgroups. Sequences from GenBank were used to design primers. Additionally, a subgroup specific ELISA was conducted for comparison. This work is part of a project which aims to develop a test for the simultaneous detection of various plant pathogens (viral, bacterial and fungal) in plant material.
KW - Cucumber mosaic virus
KW - ELISA
KW - Luminex xTAG
KW - Molecular diagnostics
UR - http://www.scopus.com/inward/record.url?scp=85012299154&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2017.01.011
DO - 10.1016/j.jviromet.2017.01.011
M3 - Article
C2 - 28109843
AN - SCOPUS:85012299154
VL - 243
SP - 35
EP - 43
JO - Journal of virological methods
JF - Journal of virological methods
SN - 0166-0934
ER -