Details
| Original language | English |
|---|---|
| Article number | 55 |
| Journal | Separations |
| Volume | 6 |
| Issue number | 4 |
| Early online date | 22 Nov 2019 |
| Publication status | Published - Dec 2019 |
Abstract
Continuous chromatography can surmount the disadvantages of batch chromatography like low productivities and extensive usage of consumables. In this work, a 4-column continuous chromatographic system based on the principle of periodic counter-current chromatography (PCCC) was developed and tested with a model protein mixture of BSA and lysozyme. The PCCC system was specially designed for membrane adsorbers as an alternative to conventional columns to facilitate the use of disposable process units and to further increase the productivity due to higher convective mass transport in the membrane adsorber. Membrane adsorber Sartobind® Q was used to continuously purify BSA from the protein mixture. The usage of PCCC led to an increased capacity utilization (here 20%) and higher space–time-yields, and thus to a remarkable productivity increase and cost savings.
Keywords
- Continuous chromatography, Membrane adsorber, Periodic counter-current chromatography
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Chemical Engineering(all)
- Filtration and Separation
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In: Separations, Vol. 6, No. 4, 55, 12.2019.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development and Testing of a 4-Columns Periodic Counter-Current Chromatography System Based on Membrane Adsorbers
AU - Brämer, Chantal
AU - Lammers, Frank
AU - Scheper, Thomas
AU - Beutel, Sascha
N1 - Funding information: Part of this work was funded by the German Federal Ministry of Education and Research (BMBF, funding number: 031B0463C). Acknowledgments: The authors would like to thank Laura Niemeyer for her work on Blockly GUI. The publication of this article was funded by the Open Access fund of Leibniz Universität Hannover.
PY - 2019/12
Y1 - 2019/12
N2 - Continuous chromatography can surmount the disadvantages of batch chromatography like low productivities and extensive usage of consumables. In this work, a 4-column continuous chromatographic system based on the principle of periodic counter-current chromatography (PCCC) was developed and tested with a model protein mixture of BSA and lysozyme. The PCCC system was specially designed for membrane adsorbers as an alternative to conventional columns to facilitate the use of disposable process units and to further increase the productivity due to higher convective mass transport in the membrane adsorber. Membrane adsorber Sartobind® Q was used to continuously purify BSA from the protein mixture. The usage of PCCC led to an increased capacity utilization (here 20%) and higher space–time-yields, and thus to a remarkable productivity increase and cost savings.
AB - Continuous chromatography can surmount the disadvantages of batch chromatography like low productivities and extensive usage of consumables. In this work, a 4-column continuous chromatographic system based on the principle of periodic counter-current chromatography (PCCC) was developed and tested with a model protein mixture of BSA and lysozyme. The PCCC system was specially designed for membrane adsorbers as an alternative to conventional columns to facilitate the use of disposable process units and to further increase the productivity due to higher convective mass transport in the membrane adsorber. Membrane adsorber Sartobind® Q was used to continuously purify BSA from the protein mixture. The usage of PCCC led to an increased capacity utilization (here 20%) and higher space–time-yields, and thus to a remarkable productivity increase and cost savings.
KW - Continuous chromatography
KW - Membrane adsorber
KW - Periodic counter-current chromatography
UR - http://www.scopus.com/inward/record.url?scp=85076500602&partnerID=8YFLogxK
U2 - 10.3390/separations6040055
DO - 10.3390/separations6040055
M3 - Article
AN - SCOPUS:85076500602
VL - 6
JO - Separations
JF - Separations
IS - 4
M1 - 55
ER -