Details
| Original language | English |
|---|---|
| Pages (from-to) | 374-390 |
| Number of pages | 17 |
| Journal | Journal of Cardiovascular Translational Research |
| Volume | 10 |
| Issue number | 4 |
| Early online date | 1 May 2017 |
| Publication status | Published - Aug 2017 |
Abstract
Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.
Keywords
- Biochemistry, Biocompatibility, Biomechanics, Collagen IV, Cytotoxicity, Decellularization, Heart valve replacement, Histology, Human adipose-derived stem cells, Human colony-forming endothelial cells, Human foreskin fibroblasts, Immunohistochemistry, Mitral valve, Scaffold, Scaffold seeding, Scanning electron microscopy, Tissue engineering, Transmission electron microscopy, Xenoepitope, α-Gal
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmaceutical Science
- Medicine(all)
- Cardiology and Cardiovascular Medicine
- Medicine(all)
- Genetics(clinical)
Sustainable Development Goals
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In: Journal of Cardiovascular Translational Research, Vol. 10, No. 4, 08.2017, p. 374-390.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering
AU - Granados, M.
AU - Morticelli, L.
AU - Andriopoulou, S.
AU - Kalozoumis, P.
AU - Pflaum, M.
AU - Iablonskii, P.
AU - Glasmacher, Birgit
AU - Harder, M.
AU - Hegermann, Jan
AU - Wrede, C.
AU - Tudorache, I.
AU - Cebotari, S.
AU - Hilfiker, A.
AU - Haverich, A.
AU - Korossis, Sotirios
N1 - Funding Information: Acknowledgements The authors would like to acknowledge the contribution of Karin Burgwitz and Annemarie Beck at the Lower Saxony Centre for Biomedical Engineering Implant Research and Development for their technical support on histological and immunohistochemical analysis. This research was supported by the People Programme (Marie Curie Actions) of the EU 7th Framework Programme FP7/2007–2013/ under the REA grant agreement number 317512, and the German Research Foundation through the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy; EXC 62). Funding Information: The authors would like to acknowledge the contribution of Karin Burgwitz and Annemarie Beck at the Lower Saxony Centre for Biomedical Engineering Implant Research and Development for their technical support on histological and immunohistochemical analysis. This research was supported by the People Programme (Marie Curie Actions) of the EU 7th Framework Programme FP7/2007?2013/ under the REA grant agreement number 317512, and the German Research Foundation through the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy; EXC 62). Publisher Copyright: © 2017, Springer Science+Business Media New York. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2017/8
Y1 - 2017/8
N2 - Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.
AB - Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.
KW - Biochemistry
KW - Biocompatibility
KW - Biomechanics
KW - Collagen IV
KW - Cytotoxicity
KW - Decellularization
KW - Heart valve replacement
KW - Histology
KW - Human adipose-derived stem cells
KW - Human colony-forming endothelial cells
KW - Human foreskin fibroblasts
KW - Immunohistochemistry
KW - Mitral valve
KW - Scaffold
KW - Scaffold seeding
KW - Scanning electron microscopy
KW - Tissue engineering
KW - Transmission electron microscopy
KW - Xenoepitope
KW - α-Gal
UR - http://www.scopus.com/inward/record.url?scp=85018361266&partnerID=8YFLogxK
U2 - 10.1007/s12265-017-9747-z
DO - 10.1007/s12265-017-9747-z
M3 - Article
C2 - 28462436
AN - SCOPUS:85018361266
VL - 10
SP - 374
EP - 390
JO - Journal of Cardiovascular Translational Research
JF - Journal of Cardiovascular Translational Research
SN - 1937-5387
IS - 4
ER -