Details
| Original language | English |
|---|---|
| Article number | 51 |
| Journal | Bioengineering |
| Volume | 4 |
| Issue number | 2 |
| Publication status | Published - 25 May 2017 |
| Externally published | Yes |
Abstract
The three dimensional (3D) cultivation of stem cells in dynamic bioreactor systems is essential in the context of regenerative medicine. Still, there is a lack of bioreactor systems that allow the cultivation of multiple independent samples under different conditions while ensuring comprehensive control over the mechanical environment. Therefore, we developed a miniaturized, parallelizable perfusion bioreactor system with two different bioreactor chambers. Pressure sensors were also implemented to determine the permeability of biomaterials which allows us to approximate the shear stress conditions. To characterize the flow velocity and shear stress profile of a porous scaffold in both bioreactor chambers, a computational fluid dynamics analysis was performed. Furthermore, the mixing behavior was characterized by acquisition of the residence time distributions. Finally, the effects of the different flow and shear stress profiles of the bioreactor chambers on osteogenic differentiation of human mesenchymal stem cells were evaluated in a proof of concept study. In conclusion, the data from computational fluid dynamics and shear stress calculations were found to be predictable for relative comparison of the bioreactor geometries, but not for final determination of the optimal flow rate. However, we suggest that the system is beneficial for parallel dynamic cultivation of multiple samples for 3D cell culture processes.
Keywords
- 3D cell culture, Computational fluid dynamics, Dynamic cultivation, Fluid shear stress, Perfusion bioreactor system
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
Sustainable Development Goals
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In: Bioengineering, Vol. 4, No. 2, 51, 25.05.2017.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development and Characterization of a Parallelizable Perfusion Bioreactor for 3D Cell Culture
AU - Egger, Dominik
AU - Fischer, Monica
AU - Hansmann, Jan
AU - Kasper, Cornelia
AU - Clementi, Andreas
AU - Ribitsch, Volker
PY - 2017/5/25
Y1 - 2017/5/25
N2 - The three dimensional (3D) cultivation of stem cells in dynamic bioreactor systems is essential in the context of regenerative medicine. Still, there is a lack of bioreactor systems that allow the cultivation of multiple independent samples under different conditions while ensuring comprehensive control over the mechanical environment. Therefore, we developed a miniaturized, parallelizable perfusion bioreactor system with two different bioreactor chambers. Pressure sensors were also implemented to determine the permeability of biomaterials which allows us to approximate the shear stress conditions. To characterize the flow velocity and shear stress profile of a porous scaffold in both bioreactor chambers, a computational fluid dynamics analysis was performed. Furthermore, the mixing behavior was characterized by acquisition of the residence time distributions. Finally, the effects of the different flow and shear stress profiles of the bioreactor chambers on osteogenic differentiation of human mesenchymal stem cells were evaluated in a proof of concept study. In conclusion, the data from computational fluid dynamics and shear stress calculations were found to be predictable for relative comparison of the bioreactor geometries, but not for final determination of the optimal flow rate. However, we suggest that the system is beneficial for parallel dynamic cultivation of multiple samples for 3D cell culture processes.
AB - The three dimensional (3D) cultivation of stem cells in dynamic bioreactor systems is essential in the context of regenerative medicine. Still, there is a lack of bioreactor systems that allow the cultivation of multiple independent samples under different conditions while ensuring comprehensive control over the mechanical environment. Therefore, we developed a miniaturized, parallelizable perfusion bioreactor system with two different bioreactor chambers. Pressure sensors were also implemented to determine the permeability of biomaterials which allows us to approximate the shear stress conditions. To characterize the flow velocity and shear stress profile of a porous scaffold in both bioreactor chambers, a computational fluid dynamics analysis was performed. Furthermore, the mixing behavior was characterized by acquisition of the residence time distributions. Finally, the effects of the different flow and shear stress profiles of the bioreactor chambers on osteogenic differentiation of human mesenchymal stem cells were evaluated in a proof of concept study. In conclusion, the data from computational fluid dynamics and shear stress calculations were found to be predictable for relative comparison of the bioreactor geometries, but not for final determination of the optimal flow rate. However, we suggest that the system is beneficial for parallel dynamic cultivation of multiple samples for 3D cell culture processes.
KW - 3D cell culture
KW - Computational fluid dynamics
KW - Dynamic cultivation
KW - Fluid shear stress
KW - Perfusion bioreactor system
UR - http://www.scopus.com/inward/record.url?scp=85028876837&partnerID=8YFLogxK
U2 - 10.3390/bioengineering4020051
DO - 10.3390/bioengineering4020051
M3 - Article
AN - SCOPUS:85028876837
VL - 4
JO - Bioengineering
JF - Bioengineering
IS - 2
M1 - 51
ER -