Details
| Original language | English |
|---|---|
| Pages (from-to) | 252-264 |
| Number of pages | 13 |
| Journal | Engineering in life sciences |
| Volume | 20 |
| Issue number | 7 |
| Publication status | Published - 6 Jul 2020 |
Abstract
The growth of microorganisms on surfaces and interfaces as a biofilm is very common and plays important role in various areas such as material science, biomedicine, or waste treatment among others. Due to their inhomogeneous structure and the variance in the microorganism consortium, the analysis of biofilms represents a significant challenge. An online fluorescence sensor was developed that is able to measure the most important biological fluorophores (proteins, nicotinamide adenine dinucleotide, and flavin) in a noninvasive manner in biofilms, e.g. in bioelectrochemical applications. The sensor gives the opportunity to continuously draw conclusions on the metabolic state of the biofilm. The developed sensor has a diameter of 1 mm at the sensor tip and can be moved on and into the biofilm surface. In the first experiment, the measuring range of the sensor and the long-term stability could be determined and the system applicability was confirmed. In addition, measurements in biofilm-like structures could be performed. The formation of a wastewater-based biofilm was monitored using the developed sensor, demonstrating the functionality of the sensor in a proof-of-principle experiment.
Keywords
- biofilm analysis, biogenic fluorophores, fluorescence sensor, online monitoring
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Environmental Science(all)
- Environmental Engineering
- Chemical Engineering(all)
- Bioengineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Engineering in life sciences, Vol. 20, No. 7, 06.07.2020, p. 252-264.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development and characterization of a fiber optical fluorescence sensor for the online monitoring of biofilms and their microenvironment
AU - Schlaugat, Jana
AU - Patzer, Kai
AU - Hentrop, Thorleif
AU - Solle, Dörte
AU - Pepelanova, Iliyana
AU - Schröder, Uwe
AU - Scheper, Thomas
PY - 2020/7/6
Y1 - 2020/7/6
N2 - The growth of microorganisms on surfaces and interfaces as a biofilm is very common and plays important role in various areas such as material science, biomedicine, or waste treatment among others. Due to their inhomogeneous structure and the variance in the microorganism consortium, the analysis of biofilms represents a significant challenge. An online fluorescence sensor was developed that is able to measure the most important biological fluorophores (proteins, nicotinamide adenine dinucleotide, and flavin) in a noninvasive manner in biofilms, e.g. in bioelectrochemical applications. The sensor gives the opportunity to continuously draw conclusions on the metabolic state of the biofilm. The developed sensor has a diameter of 1 mm at the sensor tip and can be moved on and into the biofilm surface. In the first experiment, the measuring range of the sensor and the long-term stability could be determined and the system applicability was confirmed. In addition, measurements in biofilm-like structures could be performed. The formation of a wastewater-based biofilm was monitored using the developed sensor, demonstrating the functionality of the sensor in a proof-of-principle experiment.
AB - The growth of microorganisms on surfaces and interfaces as a biofilm is very common and plays important role in various areas such as material science, biomedicine, or waste treatment among others. Due to their inhomogeneous structure and the variance in the microorganism consortium, the analysis of biofilms represents a significant challenge. An online fluorescence sensor was developed that is able to measure the most important biological fluorophores (proteins, nicotinamide adenine dinucleotide, and flavin) in a noninvasive manner in biofilms, e.g. in bioelectrochemical applications. The sensor gives the opportunity to continuously draw conclusions on the metabolic state of the biofilm. The developed sensor has a diameter of 1 mm at the sensor tip and can be moved on and into the biofilm surface. In the first experiment, the measuring range of the sensor and the long-term stability could be determined and the system applicability was confirmed. In addition, measurements in biofilm-like structures could be performed. The formation of a wastewater-based biofilm was monitored using the developed sensor, demonstrating the functionality of the sensor in a proof-of-principle experiment.
KW - biofilm analysis
KW - biogenic fluorophores
KW - fluorescence sensor
KW - online monitoring
UR - http://www.scopus.com/inward/record.url?scp=85080990411&partnerID=8YFLogxK
U2 - 10.1002/elsc.201900140
DO - 10.1002/elsc.201900140
M3 - Article
C2 - 32647504
AN - SCOPUS:85080990411
VL - 20
SP - 252
EP - 264
JO - Engineering in life sciences
JF - Engineering in life sciences
SN - 1618-0240
IS - 7
ER -