Details
| Original language | English |
|---|---|
| Pages (from-to) | 187-193 |
| Number of pages | 7 |
| Journal | Journal of Chromatography A |
| Volume | 817 |
| Issue number | 1-2 |
| Publication status | Published - 21 Aug 1998 |
Abstract
In our present work we present the determination of bovine lactoferrin in whey concentrates as they are typically produced by milk and cheese industry after production of cheese. Due to the high total protein content the analysis of whey concentrate samples is difficult and even not possible by using capillary zone electrophoresis with UV detection. To enhance the detection sensitivity we applied a more promising approach by using affinity interactions in combination with laser-induced fluorescence detection. By mixing fluoresceine isothiocyanate (FITC)-conjugated polyanionic lipopolysaccharide with the mostly positively charged lactoferrin we found a significant migration time shift which is clearly dependent on the concentration of the added protein. In the second approach we developed an immunoassay using FITC-conjugated specific antibody against bovine lactoferrin. The results of the immunoassay measurements were compared with data obtained by standard enzyme-linked immunosorbent assay analysis. Copyright (C) 1998 Elsevier Science B.V.
Keywords
- Affinity capillary electrophoresis, Immunoassays, Lactoferrin, Proteins, Whey
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Organic Chemistry
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of Chromatography A, Vol. 817, No. 1-2, 21.08.1998, p. 187-193.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Determination of the minor whey protein bovine lactoferrin in cheese whey concentrates with capillary electrophoresis
AU - Riechel, Peter
AU - Weiss, Torsten
AU - Weiss, Martina
AU - Ulber, Roland
AU - Heinrich Buchholz, Buchholz
AU - Scheper, Thomas
PY - 1998/8/21
Y1 - 1998/8/21
N2 - In our present work we present the determination of bovine lactoferrin in whey concentrates as they are typically produced by milk and cheese industry after production of cheese. Due to the high total protein content the analysis of whey concentrate samples is difficult and even not possible by using capillary zone electrophoresis with UV detection. To enhance the detection sensitivity we applied a more promising approach by using affinity interactions in combination with laser-induced fluorescence detection. By mixing fluoresceine isothiocyanate (FITC)-conjugated polyanionic lipopolysaccharide with the mostly positively charged lactoferrin we found a significant migration time shift which is clearly dependent on the concentration of the added protein. In the second approach we developed an immunoassay using FITC-conjugated specific antibody against bovine lactoferrin. The results of the immunoassay measurements were compared with data obtained by standard enzyme-linked immunosorbent assay analysis. Copyright (C) 1998 Elsevier Science B.V.
AB - In our present work we present the determination of bovine lactoferrin in whey concentrates as they are typically produced by milk and cheese industry after production of cheese. Due to the high total protein content the analysis of whey concentrate samples is difficult and even not possible by using capillary zone electrophoresis with UV detection. To enhance the detection sensitivity we applied a more promising approach by using affinity interactions in combination with laser-induced fluorescence detection. By mixing fluoresceine isothiocyanate (FITC)-conjugated polyanionic lipopolysaccharide with the mostly positively charged lactoferrin we found a significant migration time shift which is clearly dependent on the concentration of the added protein. In the second approach we developed an immunoassay using FITC-conjugated specific antibody against bovine lactoferrin. The results of the immunoassay measurements were compared with data obtained by standard enzyme-linked immunosorbent assay analysis. Copyright (C) 1998 Elsevier Science B.V.
KW - Affinity capillary electrophoresis
KW - Immunoassays
KW - Lactoferrin
KW - Proteins
KW - Whey
UR - http://www.scopus.com/inward/record.url?scp=0032555704&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(98)00445-2
DO - 10.1016/S0021-9673(98)00445-2
M3 - Article
C2 - 9764493
AN - SCOPUS:0032555704
VL - 817
SP - 187
EP - 193
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -