Details
| Original language | English |
|---|---|
| Pages (from-to) | 323-327 |
| Number of pages | 5 |
| Journal | Current Directions in Biomedical Engineering |
| Volume | 2 |
| Issue number | 1 |
| Early online date | 30 Sept 2016 |
| Publication status | Published - Sept 2016 |
Abstract
A successful cryopreservation is based on knowledge of the optimal cooling rate. So far, this is often determined by way of complex parameter studies. Alternatively, the identification of cell specific characteristics, such as osmotic behaviour, membrane hydraulic permeability and activation energy could be used to calculate the optimal cooling rate. These parameters should be determined for supra-zero and sub-zero temperatures. In this study cryomicroscopy was used. Mesenchymal stromal cells (MSCs) from bone marrow were analysed. The determined membrane hydraulic permeability for sub-zero temperatures is significantly lower than that for supra-zero temperatures. On the contrary the activation energy is significantly higher in the presence of ice. The addition of a cry-oprotective agent (CPA) such as dimethyl sulfoxid (DMSO) shows an additional influence on the characteristics of the membrane of the cell. The optimal cooling rate was determined with these parameters. For cryopreservation without DMSO the optimal cooling rate was found to be 12.82 K/min. If the MSCs were frozen with 5% (v/v) DMSO the optimal cooling rate is 16.25 K/min.
Keywords
- Cryopreservation, Membrane hydraulic permeability, Mesenchymal stromal cells, Optimal cooling rate
ASJC Scopus subject areas
- Engineering(all)
- Biomedical Engineering
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In: Current Directions in Biomedical Engineering, Vol. 2, No. 1, 09.2016, p. 323-327.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Determination of the membrane hydraulic permeability of MSCs
AU - Lopez, Jennifer Contreras
AU - Lauterböck, Lothar
AU - Glasmacher, Birgit
PY - 2016/9
Y1 - 2016/9
N2 - A successful cryopreservation is based on knowledge of the optimal cooling rate. So far, this is often determined by way of complex parameter studies. Alternatively, the identification of cell specific characteristics, such as osmotic behaviour, membrane hydraulic permeability and activation energy could be used to calculate the optimal cooling rate. These parameters should be determined for supra-zero and sub-zero temperatures. In this study cryomicroscopy was used. Mesenchymal stromal cells (MSCs) from bone marrow were analysed. The determined membrane hydraulic permeability for sub-zero temperatures is significantly lower than that for supra-zero temperatures. On the contrary the activation energy is significantly higher in the presence of ice. The addition of a cry-oprotective agent (CPA) such as dimethyl sulfoxid (DMSO) shows an additional influence on the characteristics of the membrane of the cell. The optimal cooling rate was determined with these parameters. For cryopreservation without DMSO the optimal cooling rate was found to be 12.82 K/min. If the MSCs were frozen with 5% (v/v) DMSO the optimal cooling rate is 16.25 K/min.
AB - A successful cryopreservation is based on knowledge of the optimal cooling rate. So far, this is often determined by way of complex parameter studies. Alternatively, the identification of cell specific characteristics, such as osmotic behaviour, membrane hydraulic permeability and activation energy could be used to calculate the optimal cooling rate. These parameters should be determined for supra-zero and sub-zero temperatures. In this study cryomicroscopy was used. Mesenchymal stromal cells (MSCs) from bone marrow were analysed. The determined membrane hydraulic permeability for sub-zero temperatures is significantly lower than that for supra-zero temperatures. On the contrary the activation energy is significantly higher in the presence of ice. The addition of a cry-oprotective agent (CPA) such as dimethyl sulfoxid (DMSO) shows an additional influence on the characteristics of the membrane of the cell. The optimal cooling rate was determined with these parameters. For cryopreservation without DMSO the optimal cooling rate was found to be 12.82 K/min. If the MSCs were frozen with 5% (v/v) DMSO the optimal cooling rate is 16.25 K/min.
KW - Cryopreservation
KW - Membrane hydraulic permeability
KW - Mesenchymal stromal cells
KW - Optimal cooling rate
UR - http://www.scopus.com/inward/record.url?scp=85059811825&partnerID=8YFLogxK
U2 - 10.1515/cdbme-2016-0072
DO - 10.1515/cdbme-2016-0072
M3 - Article
AN - SCOPUS:85059811825
VL - 2
SP - 323
EP - 327
JO - Current Directions in Biomedical Engineering
JF - Current Directions in Biomedical Engineering
IS - 1
ER -