Details
| Original language | English |
|---|---|
| Pages (from-to) | 10-12 |
| Number of pages | 3 |
| Journal | Analytical biochemistry |
| Volume | 455 |
| Issue number | 1 |
| Publication status | Published - 20 Mar 2014 |
Abstract
In the current work, the quantification of different poly(ethylene glycol) (PEG)-potassium phosphate/sodium citrate aqueous two-phase system (ATPS) phase-forming components was investigated by using conductivity and refractive index measurements. For this purpose, refractive index and conductivity calibration curves were obtained for ATPS at different pH values in the presence of different bovine serum albumin (BSA) concentrations. Whereas BSA had no effect on the conductivity, it had a considerable effect on the refractive index. Finally, a convenient dilution of the samples prior to the ATPS constituent determination is needed to ensure no significant influence from BSA.
Keywords
- Aqueous two-phase system, Conductivity measurement, Refractive index measurement
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Analytical biochemistry, Vol. 455, No. 1, 20.03.2014, p. 10-12.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Determination of aqueous two-phase system phase-forming components in the presence of bovine serum albumin
AU - Glyk, Anna
AU - Scheper, Thomas
AU - Beutel, Sascha
PY - 2014/3/20
Y1 - 2014/3/20
N2 - In the current work, the quantification of different poly(ethylene glycol) (PEG)-potassium phosphate/sodium citrate aqueous two-phase system (ATPS) phase-forming components was investigated by using conductivity and refractive index measurements. For this purpose, refractive index and conductivity calibration curves were obtained for ATPS at different pH values in the presence of different bovine serum albumin (BSA) concentrations. Whereas BSA had no effect on the conductivity, it had a considerable effect on the refractive index. Finally, a convenient dilution of the samples prior to the ATPS constituent determination is needed to ensure no significant influence from BSA.
AB - In the current work, the quantification of different poly(ethylene glycol) (PEG)-potassium phosphate/sodium citrate aqueous two-phase system (ATPS) phase-forming components was investigated by using conductivity and refractive index measurements. For this purpose, refractive index and conductivity calibration curves were obtained for ATPS at different pH values in the presence of different bovine serum albumin (BSA) concentrations. Whereas BSA had no effect on the conductivity, it had a considerable effect on the refractive index. Finally, a convenient dilution of the samples prior to the ATPS constituent determination is needed to ensure no significant influence from BSA.
KW - Aqueous two-phase system
KW - Conductivity measurement
KW - Refractive index measurement
UR - http://www.scopus.com/inward/record.url?scp=84899446959&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2014.03.009
DO - 10.1016/j.ab.2014.03.009
M3 - Article
C2 - 24657359
AN - SCOPUS:84899446959
VL - 455
SP - 10
EP - 12
JO - Analytical biochemistry
JF - Analytical biochemistry
SN - 0003-2697
IS - 1
ER -