Details
| Original language | English |
|---|---|
| Article number | 897 |
| Journal | Plants |
| Volume | 10 |
| Issue number | 5 |
| Publication status | Published - 29 Apr 2021 |
Abstract
The detection of QTL by association genetics depends on the genetic architecture of the trait under study, the size and structure of the investigated population and the availability of phenotypic and marker data of sufficient quality and quantity. In roses, we previously demonstrated that major QTL could already be detected in small association panels. In this study, we analyzed petal number, petal size and fragrance in a small panel of 95 mostly tetraploid garden rose genotypes. After genotyping the panel with the 68 K Axiom WagRhSNP chip we detected major QTL for all three traits. Each trait was significantly influenced by several genomic regions. Some of the QTL span genomic regions that comprise several candidate genes. Selected markers from some of these regions were converted into KASP markers and were validated in independent populations of up to 282 garden rose genotypes. These markers demonstrate the robustness of the detected effects independent of the set of genotypes analyzed. Furthermore, the markers can serve as tools for marker-assisted breeding in garden roses. Over an extended timeframe, they may be used as a starting point for the isolation of the genes underlying the QTL.
Keywords
- Association mapping, Flower QTL, Fragrance, KASP, Marker-assisted selection, Petal number, Petal size, SNP
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Agricultural and Biological Sciences(all)
- Plant Science
- Environmental Science(all)
- Ecology
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In: Plants, Vol. 10, No. 5, 897, 29.04.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Detection of Reproducible Major Effect QTL for Petal Traits in Garden Roses
AU - Schulz, Dietmar
AU - Linde, Marcus
AU - Debener, Thomas
N1 - Funding Information: Funding: This research was supported by a grant from the Aif Projekt GmbH (FKZ: KF2554805MD4) The publication of this article was funded by the Open Access fund of the Leibniz Universität Hannover.
PY - 2021/4/29
Y1 - 2021/4/29
N2 - The detection of QTL by association genetics depends on the genetic architecture of the trait under study, the size and structure of the investigated population and the availability of phenotypic and marker data of sufficient quality and quantity. In roses, we previously demonstrated that major QTL could already be detected in small association panels. In this study, we analyzed petal number, petal size and fragrance in a small panel of 95 mostly tetraploid garden rose genotypes. After genotyping the panel with the 68 K Axiom WagRhSNP chip we detected major QTL for all three traits. Each trait was significantly influenced by several genomic regions. Some of the QTL span genomic regions that comprise several candidate genes. Selected markers from some of these regions were converted into KASP markers and were validated in independent populations of up to 282 garden rose genotypes. These markers demonstrate the robustness of the detected effects independent of the set of genotypes analyzed. Furthermore, the markers can serve as tools for marker-assisted breeding in garden roses. Over an extended timeframe, they may be used as a starting point for the isolation of the genes underlying the QTL.
AB - The detection of QTL by association genetics depends on the genetic architecture of the trait under study, the size and structure of the investigated population and the availability of phenotypic and marker data of sufficient quality and quantity. In roses, we previously demonstrated that major QTL could already be detected in small association panels. In this study, we analyzed petal number, petal size and fragrance in a small panel of 95 mostly tetraploid garden rose genotypes. After genotyping the panel with the 68 K Axiom WagRhSNP chip we detected major QTL for all three traits. Each trait was significantly influenced by several genomic regions. Some of the QTL span genomic regions that comprise several candidate genes. Selected markers from some of these regions were converted into KASP markers and were validated in independent populations of up to 282 garden rose genotypes. These markers demonstrate the robustness of the detected effects independent of the set of genotypes analyzed. Furthermore, the markers can serve as tools for marker-assisted breeding in garden roses. Over an extended timeframe, they may be used as a starting point for the isolation of the genes underlying the QTL.
KW - Association mapping
KW - Flower QTL
KW - Fragrance
KW - KASP
KW - Marker-assisted selection
KW - Petal number
KW - Petal size
KW - SNP
UR - http://www.scopus.com/inward/record.url?scp=85129684140&partnerID=8YFLogxK
U2 - 10.3390/plants10050897
DO - 10.3390/plants10050897
M3 - Article
C2 - 33946713
VL - 10
JO - Plants
JF - Plants
SN - 2223-7747
IS - 5
M1 - 897
ER -