Details
| Original language | English |
|---|---|
| Pages (from-to) | 619-626 |
| Number of pages | 8 |
| Journal | Journal of phytopathology |
| Volume | 148 |
| Issue number | 11-12 |
| Publication status | Published - 2000 |
Abstract
A new polymerase chain reaction (PCR) assay was developed for the detection of Ralstonia solanacearum in potato tubers. The designed primers PS-1/PS-2 based on the sequence data of the 16S rRNA gene. Using the optimized PCR protocol, it was possible to detect R. solanacearum cells artificially added to concentrated potato extracts in the range of 1-10 colony-forming units (CFU) per PCR reaction mixture (10-100 CFU/ml potato homogenate). No amplification products were obtained, when bacteria belonging to other species or genera were submitted to PCR under the same conditions. A total of 10 different DNA extraction methods were adapted for the isolation of R. solanacearum DNA from potato homogenates and were compared for their suitability as pre-PCR procedures.
Keywords
- DNA extraction, Polymerase chain reaction, Ralstonia solanacearum
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of phytopathology, Vol. 148, No. 11-12, 2000, p. 619-626.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Detection of Ralstonia solanacearum in potato tubers by polymerase chain reaction
AU - Pastrik, K. H.
AU - Maiss, E.
PY - 2000
Y1 - 2000
N2 - A new polymerase chain reaction (PCR) assay was developed for the detection of Ralstonia solanacearum in potato tubers. The designed primers PS-1/PS-2 based on the sequence data of the 16S rRNA gene. Using the optimized PCR protocol, it was possible to detect R. solanacearum cells artificially added to concentrated potato extracts in the range of 1-10 colony-forming units (CFU) per PCR reaction mixture (10-100 CFU/ml potato homogenate). No amplification products were obtained, when bacteria belonging to other species or genera were submitted to PCR under the same conditions. A total of 10 different DNA extraction methods were adapted for the isolation of R. solanacearum DNA from potato homogenates and were compared for their suitability as pre-PCR procedures.
AB - A new polymerase chain reaction (PCR) assay was developed for the detection of Ralstonia solanacearum in potato tubers. The designed primers PS-1/PS-2 based on the sequence data of the 16S rRNA gene. Using the optimized PCR protocol, it was possible to detect R. solanacearum cells artificially added to concentrated potato extracts in the range of 1-10 colony-forming units (CFU) per PCR reaction mixture (10-100 CFU/ml potato homogenate). No amplification products were obtained, when bacteria belonging to other species or genera were submitted to PCR under the same conditions. A total of 10 different DNA extraction methods were adapted for the isolation of R. solanacearum DNA from potato homogenates and were compared for their suitability as pre-PCR procedures.
KW - DNA extraction
KW - Polymerase chain reaction
KW - Ralstonia solanacearum
UR - http://www.scopus.com/inward/record.url?scp=0034463288&partnerID=8YFLogxK
U2 - 10.1046/j.1439-0434.2000.00567.x
DO - 10.1046/j.1439-0434.2000.00567.x
M3 - Article
AN - SCOPUS:0034463288
VL - 148
SP - 619
EP - 626
JO - Journal of phytopathology
JF - Journal of phytopathology
SN - 0931-1785
IS - 11-12
ER -