Details
| Original language | English |
|---|---|
| Title of host publication | Optical Biopsy XXI |
| Subtitle of host publication | Toward Real-Time Spectroscopic Imaging and Diagnosis |
| Editors | Robert R. Alfano, Angela B. Seddon |
| Publisher | SPIE |
| ISBN (electronic) | 9781510658516 |
| Publication status | Published - 6 Mar 2023 |
| Event | Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis 2023 - San Francisco, United States Duration: 28 Jan 2023 → 3 Feb 2023 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 12373 |
| ISSN (Print) | 1605-7422 |
Abstract
Melanoma skin cancer is one of the most dangerous types of cancer and responsible for more than half of the skin cancer-related deaths. Common methods for skin cancer detection are dermoscopy and visual inspection, the accuracy of which is greatly influenced by the training and experience of the physician. In some extreme cases, histological findings, which are considered the gold standard for detecting skin cancer, might also cause a debate among competent clinicians. To achieve accurate non-invasive skin cancer diagnostics, a dual-mode optical biopsy system made up of a Raman spectrometer and an OCT system was built. OCT morphological images enable precise localization of the position and depth of lesions for subsequent Raman detection. Skin models were generated by dissolving synthetic melanin in dimethyl sulfoxide (DMSO) and applying it to fresh porcine skin samples to investigate the influence of melanin concentration on skin spectra. The amide-I band and the CH2-deformation band detected by using Raman spectroscopy had a lower intensity in the spectra of the samples treated with melanin, which may imply that the addition of melanin promotes the breakdown of proteins and lipids. The intensities of the CH2 twist and C-C stretch bands increased compared to the drop of the aforesaid bands. Given that these two structures are often present in phospholipids, which are the fundamental components of cell membranes, one possible explanation is that melanin has less influence on membranes than on biological macromolecules.
Keywords
- dual-modality optical system, melanin in skin, optical coherence tomography, Raman spectroscopy, skin cancer diagnostics
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Biomaterials
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Sustainable Development Goals
Cite this
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- Apa
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- BibTeX
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Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis. ed. / Robert R. Alfano; Angela B. Seddon. SPIE, 2023. 1237309 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12373).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Detection of Cutaneous Melanin based on Raman Spectroscopy with Optical Coherence Tomography Localization
AU - Wu, Di
AU - Fedorov Kukk, Anatoly
AU - Roth, Bernhard
N1 - Funding Information: The authors acknowledge financial support from the German Research Foundation DFG (German Research Foundation, Project ID RO 3471/18-1 and EM 63/13-1). Also, financial support from the German Research Foundation (DFG) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453) is acknowledged.
PY - 2023/3/6
Y1 - 2023/3/6
N2 - Melanoma skin cancer is one of the most dangerous types of cancer and responsible for more than half of the skin cancer-related deaths. Common methods for skin cancer detection are dermoscopy and visual inspection, the accuracy of which is greatly influenced by the training and experience of the physician. In some extreme cases, histological findings, which are considered the gold standard for detecting skin cancer, might also cause a debate among competent clinicians. To achieve accurate non-invasive skin cancer diagnostics, a dual-mode optical biopsy system made up of a Raman spectrometer and an OCT system was built. OCT morphological images enable precise localization of the position and depth of lesions for subsequent Raman detection. Skin models were generated by dissolving synthetic melanin in dimethyl sulfoxide (DMSO) and applying it to fresh porcine skin samples to investigate the influence of melanin concentration on skin spectra. The amide-I band and the CH2-deformation band detected by using Raman spectroscopy had a lower intensity in the spectra of the samples treated with melanin, which may imply that the addition of melanin promotes the breakdown of proteins and lipids. The intensities of the CH2 twist and C-C stretch bands increased compared to the drop of the aforesaid bands. Given that these two structures are often present in phospholipids, which are the fundamental components of cell membranes, one possible explanation is that melanin has less influence on membranes than on biological macromolecules.
AB - Melanoma skin cancer is one of the most dangerous types of cancer and responsible for more than half of the skin cancer-related deaths. Common methods for skin cancer detection are dermoscopy and visual inspection, the accuracy of which is greatly influenced by the training and experience of the physician. In some extreme cases, histological findings, which are considered the gold standard for detecting skin cancer, might also cause a debate among competent clinicians. To achieve accurate non-invasive skin cancer diagnostics, a dual-mode optical biopsy system made up of a Raman spectrometer and an OCT system was built. OCT morphological images enable precise localization of the position and depth of lesions for subsequent Raman detection. Skin models were generated by dissolving synthetic melanin in dimethyl sulfoxide (DMSO) and applying it to fresh porcine skin samples to investigate the influence of melanin concentration on skin spectra. The amide-I band and the CH2-deformation band detected by using Raman spectroscopy had a lower intensity in the spectra of the samples treated with melanin, which may imply that the addition of melanin promotes the breakdown of proteins and lipids. The intensities of the CH2 twist and C-C stretch bands increased compared to the drop of the aforesaid bands. Given that these two structures are often present in phospholipids, which are the fundamental components of cell membranes, one possible explanation is that melanin has less influence on membranes than on biological macromolecules.
KW - dual-modality optical system
KW - melanin in skin
KW - optical coherence tomography
KW - Raman spectroscopy
KW - skin cancer diagnostics
UR - http://www.scopus.com/inward/record.url?scp=85152767534&partnerID=8YFLogxK
U2 - 10.1117/12.2648242
DO - 10.1117/12.2648242
M3 - Conference contribution
AN - SCOPUS:85152767534
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Biopsy XXI
A2 - Alfano, Robert R.
A2 - Seddon, Angela B.
PB - SPIE
T2 - Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis 2023
Y2 - 28 January 2023 through 3 February 2023
ER -