Details
| Original language | English |
|---|---|
| Pages (from-to) | 479-483 |
| Number of pages | 5 |
| Journal | Journal of Horticultural Science and Biotechnology |
| Volume | 79 |
| Issue number | 3 |
| Early online date | 7 Nov 2015 |
| Publication status | E-pub ahead of print - 7 Nov 2015 |
Abstract
To achieve storable somatic embryos (SE) of Cyclamen persicum, different desiccation conditions and two rehydration methods were tested. Somatic embryos derived from liquid cultures were dried in various relative humidities (r.h.) using desiccators filled with saturated salt solutions. Embryos dried to moisture contents of 24% and higher were able to germinate. For germination of the dried somatic embryos, the mode of rehydration was crucial. Only after imbibition in liquid hormone-free medium tubers were cotyledons and roots formed. Drying in decreasing r.h. (91-57-18%) resulted in low moisture contents of 12% on average, comparable with that found in seeds. Depending on the quality of somatic embryos significant differences were observed between different experiments. Somatic embryos with a high dry-matter content showed germination rates of 60% after desiccation in decreasing r.h. Germinating embryos formed bigger tubers than non-dried controls and developed cotyledons of better quality.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Journal of Horticultural Science and Biotechnology, Vol. 79, No. 3, 07.11.2015, p. 479-483.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Desiccation of somatic embryos of Cyclamen persicum Mill
AU - Winkelmann, T.
AU - Meyer, L.
AU - Serek, M.
PY - 2015/11/7
Y1 - 2015/11/7
N2 - To achieve storable somatic embryos (SE) of Cyclamen persicum, different desiccation conditions and two rehydration methods were tested. Somatic embryos derived from liquid cultures were dried in various relative humidities (r.h.) using desiccators filled with saturated salt solutions. Embryos dried to moisture contents of 24% and higher were able to germinate. For germination of the dried somatic embryos, the mode of rehydration was crucial. Only after imbibition in liquid hormone-free medium tubers were cotyledons and roots formed. Drying in decreasing r.h. (91-57-18%) resulted in low moisture contents of 12% on average, comparable with that found in seeds. Depending on the quality of somatic embryos significant differences were observed between different experiments. Somatic embryos with a high dry-matter content showed germination rates of 60% after desiccation in decreasing r.h. Germinating embryos formed bigger tubers than non-dried controls and developed cotyledons of better quality.
AB - To achieve storable somatic embryos (SE) of Cyclamen persicum, different desiccation conditions and two rehydration methods were tested. Somatic embryos derived from liquid cultures were dried in various relative humidities (r.h.) using desiccators filled with saturated salt solutions. Embryos dried to moisture contents of 24% and higher were able to germinate. For germination of the dried somatic embryos, the mode of rehydration was crucial. Only after imbibition in liquid hormone-free medium tubers were cotyledons and roots formed. Drying in decreasing r.h. (91-57-18%) resulted in low moisture contents of 12% on average, comparable with that found in seeds. Depending on the quality of somatic embryos significant differences were observed between different experiments. Somatic embryos with a high dry-matter content showed germination rates of 60% after desiccation in decreasing r.h. Germinating embryos formed bigger tubers than non-dried controls and developed cotyledons of better quality.
UR - http://www.scopus.com/inward/record.url?scp=2642557362&partnerID=8YFLogxK
U2 - 10.1080/14620316.2004.11511793
DO - 10.1080/14620316.2004.11511793
M3 - Article
AN - SCOPUS:2642557362
VL - 79
SP - 479
EP - 483
JO - Journal of Horticultural Science and Biotechnology
JF - Journal of Horticultural Science and Biotechnology
SN - 1462-0316
IS - 3
ER -