Details
| Original language | English |
|---|---|
| Article number | 15 |
| Journal | Journal of nanobiotechnology |
| Volume | 11 |
| Issue number | 1 |
| Publication status | Published - 25 May 2013 |
Abstract
Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
Keywords
- Bionanoparticles, Dengue envelope domain III, Hepatitis B surface antigen, Pichia pastoris, Virus-like particle
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Medicine (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Engineering(all)
- Biomedical Engineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmaceutical Science
Sustainable Development Goals
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In: Journal of nanobiotechnology, Vol. 11, No. 1, 15, 25.05.2013.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Dengue-specific subviral nanoparticles
T2 - Design, creation and characterization
AU - Khetarpal, Niyati
AU - Poddar, Ankur
AU - Nemani, Satish K.
AU - Dhar, Nisha
AU - Patil, Aravind
AU - Negi, Priyanka
AU - Perween, Ashiya
AU - Viswanathan, Ramaswamy
AU - Lünsdorf, Heinrich
AU - Tyagi, Poornima
AU - Raut, Rajendra
AU - Arora, Upasana
AU - Jain, Swatantra K.
AU - Rinas, Ursula
AU - Swaminathan, Sathyamangalam
AU - Khanna, Navin
N1 - Funding Information: NiK and AnP are recipients of junior research fellowships from the Council of Scientific & Industrial Research, Government of India. SS and NaK are grateful to the Department of Biotechnology, Government of India for providing funds to perform this work. SS and NaK thank Drs. Harold Margolis, Dennis Trent, George Siber and Cristina Cassetti for their inputs in designing the mosaic VLPs.
PY - 2013/5/25
Y1 - 2013/5/25
N2 - Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
AB - Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
KW - Bionanoparticles
KW - Dengue envelope domain III
KW - Hepatitis B surface antigen
KW - Pichia pastoris
KW - Virus-like particle
UR - http://www.scopus.com/inward/record.url?scp=84878005407&partnerID=8YFLogxK
U2 - 10.1186/1477-3155-11-15
DO - 10.1186/1477-3155-11-15
M3 - Article
C2 - 23706089
AN - SCOPUS:84878005407
VL - 11
JO - Journal of nanobiotechnology
JF - Journal of nanobiotechnology
SN - 1477-3155
IS - 1
M1 - 15
ER -