Details
| Original language | English |
|---|---|
| Pages (from-to) | 108-114 |
| Number of pages | 7 |
| Journal | CRYOBIOLOGY |
| Volume | 61 |
| Issue number | 1 |
| Early online date | 8 Jun 2010 |
| Publication status | Published - Aug 2010 |
Abstract
According to the water replacement hypothesis, trehalose stabilizes dry membranes by preventing the decrease in spacing between adjacent phopspholipid headgroups during dehydration. Alternatively, the water-entrapment hypothesis postulates that in the dried state sugars trap residual water at the biomolecule sugar interface. In this study, Fourier transform infrared spectroscopy with an attenuated total reflection accessory was used to investigate the influence of trehalose on the dehydration kinetics and residual water content of egg phosphatidylcholine liposomes in real time under controlled relative humidity conditions. In the absence of trehalose, the lipids displayed a transition to a more ordered gel phase upon drying. The membrane conformational disorder in the dried state was found to decrease with decreasing relative humidity. Even at a relative humidity as high as 94% the conformational disorder of the lipid acyl chains decreased after evaporation of the bulk water. The presence of trehalose affects the rate of water removal from the system and the lipid phase behavior. The rate of water removal is decreased and the residual water content is higher, as compared to drying in the absence of trehalose. During drying, the level of hydrogen bonding to the head groups remains constant. In addition, the conformational disorder of the lipid acyl chains in the dried state more closely resembles that of the lipids in the fully hydrated state. We conclude that water entrapment rather than water replacement explains the effect of trehalose on lipid phase behavior of phosphatidylcholine lipid bilayers during the initial phase of drying.
Keywords
- Anhydrobiosis, Fourier transform infrared spectroscopy, Lipid phase behavior, Liposomes, Lyotropism, Trehalose, Water replacement hypothesis
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Agricultural and Biological Sciences(all)
- General Agricultural and Biological Sciences
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In: CRYOBIOLOGY, Vol. 61, No. 1, 08.2010, p. 108-114.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Dehydrating phospholipid vesicles measured in real-time using ATR Fourier transform infrared spectroscopy
AU - Wolkers, Willem F.
AU - Oldenhof, Harriëtte
AU - Glasmacher, Birgit
N1 - Funding Information: Statement of funding: This work is supported by funding from the German Research Foundation (DFG, Deutsche Forschungsgemeinschaft) for the Cluster of Excellence “From Regenerative Biology to Reconstructive Therapy” (REBIRTH).
PY - 2010/8
Y1 - 2010/8
N2 - According to the water replacement hypothesis, trehalose stabilizes dry membranes by preventing the decrease in spacing between adjacent phopspholipid headgroups during dehydration. Alternatively, the water-entrapment hypothesis postulates that in the dried state sugars trap residual water at the biomolecule sugar interface. In this study, Fourier transform infrared spectroscopy with an attenuated total reflection accessory was used to investigate the influence of trehalose on the dehydration kinetics and residual water content of egg phosphatidylcholine liposomes in real time under controlled relative humidity conditions. In the absence of trehalose, the lipids displayed a transition to a more ordered gel phase upon drying. The membrane conformational disorder in the dried state was found to decrease with decreasing relative humidity. Even at a relative humidity as high as 94% the conformational disorder of the lipid acyl chains decreased after evaporation of the bulk water. The presence of trehalose affects the rate of water removal from the system and the lipid phase behavior. The rate of water removal is decreased and the residual water content is higher, as compared to drying in the absence of trehalose. During drying, the level of hydrogen bonding to the head groups remains constant. In addition, the conformational disorder of the lipid acyl chains in the dried state more closely resembles that of the lipids in the fully hydrated state. We conclude that water entrapment rather than water replacement explains the effect of trehalose on lipid phase behavior of phosphatidylcholine lipid bilayers during the initial phase of drying.
AB - According to the water replacement hypothesis, trehalose stabilizes dry membranes by preventing the decrease in spacing between adjacent phopspholipid headgroups during dehydration. Alternatively, the water-entrapment hypothesis postulates that in the dried state sugars trap residual water at the biomolecule sugar interface. In this study, Fourier transform infrared spectroscopy with an attenuated total reflection accessory was used to investigate the influence of trehalose on the dehydration kinetics and residual water content of egg phosphatidylcholine liposomes in real time under controlled relative humidity conditions. In the absence of trehalose, the lipids displayed a transition to a more ordered gel phase upon drying. The membrane conformational disorder in the dried state was found to decrease with decreasing relative humidity. Even at a relative humidity as high as 94% the conformational disorder of the lipid acyl chains decreased after evaporation of the bulk water. The presence of trehalose affects the rate of water removal from the system and the lipid phase behavior. The rate of water removal is decreased and the residual water content is higher, as compared to drying in the absence of trehalose. During drying, the level of hydrogen bonding to the head groups remains constant. In addition, the conformational disorder of the lipid acyl chains in the dried state more closely resembles that of the lipids in the fully hydrated state. We conclude that water entrapment rather than water replacement explains the effect of trehalose on lipid phase behavior of phosphatidylcholine lipid bilayers during the initial phase of drying.
KW - Anhydrobiosis
KW - Fourier transform infrared spectroscopy
KW - Lipid phase behavior
KW - Liposomes
KW - Lyotropism
KW - Trehalose
KW - Water replacement hypothesis
UR - http://www.scopus.com/inward/record.url?scp=77955414935&partnerID=8YFLogxK
U2 - 10.1016/j.cryobiol.2010.06.001
DO - 10.1016/j.cryobiol.2010.06.001
M3 - Article
C2 - 20566369
AN - SCOPUS:77955414935
VL - 61
SP - 108
EP - 114
JO - CRYOBIOLOGY
JF - CRYOBIOLOGY
SN - 0011-2240
IS - 1
ER -