Details
| Original language | English |
|---|---|
| Pages (from-to) | 131-143 |
| Number of pages | 13 |
| Journal | BioNanoMaterials |
| Volume | 17 |
| Issue number | 3-4 |
| Early online date | 29 Apr 2016 |
| Publication status | Published - 1 Sept 2016 |
Abstract
As magnesium and many of its alloys are a promising class of degradable implant materials, a thorough understanding of their degradation under physiological conditions is a key challenge in the field of biomaterial science. In order to increase the predictive power of in vitro studies, it is necessary to imitate the in vivo conditions, track the decomposition process and identify the products that form during the degradation pathway. In this in vitro study, slices of pure magnesium were exposed to Hank's Balanced Salt Solution (HBSS), Dulbecco's Modified Eagle Medium (DMEM) and simulated body fluid (SBF), respectively, under cell culture conditions, which included CO2 gassing. The series were repeated with supplements of fetal bovine serum (FBS), added to the respective media. Degradation rates, osmolality and pH were found to vary with the choice of medium and supplementation with proteins. In order to identify the crystalline degradation products, the crusts formed on the specimens were investigated via X-ray diffraction (XRD) measurements. As expected, brucite, Mg(OH)2, was found among the degradation products; interestingly, nesquehonite, Mg(HCO3)(OH)·2H2O, was found to be the dominant degradation product in this study. The experimental data are well in accordance with solubility calculations.
Keywords
- brucite, Dulbecco's Modified Eagle Medium, Hank's Balanced Salt Solution, nesquehonite, pure magnesium, simulated body fluid
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Engineering(all)
- Biomedical Engineering
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In: BioNanoMaterials, Vol. 17, No. 3-4, 01.09.2016, p. 131-143.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Degradation rates and products of pure magnesium exposed to different aqueous media under physiological conditions
AU - Kieke, Marc
AU - Feyerabend, Frank
AU - Lemaitre, Jacques
AU - Behrens, Peter
AU - Willumeit-Römer, Regine
PY - 2016/9/1
Y1 - 2016/9/1
N2 - As magnesium and many of its alloys are a promising class of degradable implant materials, a thorough understanding of their degradation under physiological conditions is a key challenge in the field of biomaterial science. In order to increase the predictive power of in vitro studies, it is necessary to imitate the in vivo conditions, track the decomposition process and identify the products that form during the degradation pathway. In this in vitro study, slices of pure magnesium were exposed to Hank's Balanced Salt Solution (HBSS), Dulbecco's Modified Eagle Medium (DMEM) and simulated body fluid (SBF), respectively, under cell culture conditions, which included CO2 gassing. The series were repeated with supplements of fetal bovine serum (FBS), added to the respective media. Degradation rates, osmolality and pH were found to vary with the choice of medium and supplementation with proteins. In order to identify the crystalline degradation products, the crusts formed on the specimens were investigated via X-ray diffraction (XRD) measurements. As expected, brucite, Mg(OH)2, was found among the degradation products; interestingly, nesquehonite, Mg(HCO3)(OH)·2H2O, was found to be the dominant degradation product in this study. The experimental data are well in accordance with solubility calculations.
AB - As magnesium and many of its alloys are a promising class of degradable implant materials, a thorough understanding of their degradation under physiological conditions is a key challenge in the field of biomaterial science. In order to increase the predictive power of in vitro studies, it is necessary to imitate the in vivo conditions, track the decomposition process and identify the products that form during the degradation pathway. In this in vitro study, slices of pure magnesium were exposed to Hank's Balanced Salt Solution (HBSS), Dulbecco's Modified Eagle Medium (DMEM) and simulated body fluid (SBF), respectively, under cell culture conditions, which included CO2 gassing. The series were repeated with supplements of fetal bovine serum (FBS), added to the respective media. Degradation rates, osmolality and pH were found to vary with the choice of medium and supplementation with proteins. In order to identify the crystalline degradation products, the crusts formed on the specimens were investigated via X-ray diffraction (XRD) measurements. As expected, brucite, Mg(OH)2, was found among the degradation products; interestingly, nesquehonite, Mg(HCO3)(OH)·2H2O, was found to be the dominant degradation product in this study. The experimental data are well in accordance with solubility calculations.
KW - brucite
KW - Dulbecco's Modified Eagle Medium
KW - Hank's Balanced Salt Solution
KW - nesquehonite
KW - pure magnesium
KW - simulated body fluid
UR - http://www.scopus.com/inward/record.url?scp=84996508943&partnerID=8YFLogxK
U2 - 10.1515/bnm-2015-0020
DO - 10.1515/bnm-2015-0020
M3 - Article
AN - SCOPUS:84996508943
VL - 17
SP - 131
EP - 143
JO - BioNanoMaterials
JF - BioNanoMaterials
SN - 2193-0651
IS - 3-4
ER -