Cuticle deposition ceases during strawberry fruit development

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Jannis Straube
  • Grecia Hurtado
  • Viktoria Zeisler-Diehl
  • Lukas Schreiber
  • Moritz Knoche

External Research Organisations

  • University of Bonn
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Details

Original languageEnglish
Article number623
Number of pages13
JournalBMC plant biology
Volume24
Issue number1
Early online date29 Jun 2024
Publication statusE-pub ahead of print - 29 Jun 2024

Abstract

Background: Ideally, the barrier properties of a fruit’s cuticle persist throughout its development. This presents a challenge for strawberry fruit, with their rapid development and thin cuticles. The objective was to establish the developmental time course of cuticle deposition in strawberry fruit. Results: Fruit mass and surface area increase rapidly, with peak growth rate coinciding with the onset of ripening. On a whole-fruit basis, the masses of cutin and wax increase but on a unit surface-area basis, they decrease. The decrease is associated with marked increases in elastic strain. The expressions of cuticle-associated genes involved in transcriptional regulation (FaSHN1, FaSHN2, FaSHN3), synthesis of cutin (FaLACS2, FaGPAT3) and wax (FaCER1, FaKCS10, FaKCR1), and those involved in transport of cutin monomers and wax constituents (FaABCG11, FaABCG32) decreased until maturity. The only exceptions were FaLACS6 and FaGPAT6 that are presumably involved in cutin synthesis, and FaCER1 involved in wax synthesis. This result was consistent across five strawberry cultivars. Strawberry cutin consists mainly of C16 and C18 monomers, plus minor amounts of C19, C20, C22 and C24 monomers, ω-hydroxy acids, dihydroxy acids, epoxy acids, primary alcohols, carboxylic acids and dicarboxylic acids. The most abundant monomer is 10,16-dihydroxyhexadecanoic acid. Waxes comprise mainly long-chain fatty acids C29 to C46, with smaller amounts of C16 to C28. Wax constituents are carboxylic acids, primary alcohols, alkanes, aldehydes, sterols and esters. Conclusion: The downregulation of cuticle deposition during development accounts for the marked cuticular strain, for the associated microcracking, and for their high susceptibility to the disorders of water soaking and cracking.

Keywords

    Cuticle, Cutin, Fragaria x ananassa Duch, Strain, Wax

ASJC Scopus subject areas

Cite this

Cuticle deposition ceases during strawberry fruit development. / Straube, Jannis; Hurtado, Grecia; Zeisler-Diehl, Viktoria et al.
In: BMC plant biology, Vol. 24, No. 1, 623, 12.2024.

Research output: Contribution to journalArticleResearchpeer review

Straube, J, Hurtado, G, Zeisler-Diehl, V, Schreiber, L & Knoche, M 2024, 'Cuticle deposition ceases during strawberry fruit development', BMC plant biology, vol. 24, no. 1, 623. https://doi.org/10.1186/s12870-024-05327-7
Straube, J., Hurtado, G., Zeisler-Diehl, V., Schreiber, L., & Knoche, M. (2024). Cuticle deposition ceases during strawberry fruit development. BMC plant biology, 24(1), Article 623. Advance online publication. https://doi.org/10.1186/s12870-024-05327-7
Straube J, Hurtado G, Zeisler-Diehl V, Schreiber L, Knoche M. Cuticle deposition ceases during strawberry fruit development. BMC plant biology. 2024 Dec;24(1):623. Epub 2024 Jun 29. doi: 10.1186/s12870-024-05327-7
Straube, Jannis ; Hurtado, Grecia ; Zeisler-Diehl, Viktoria et al. / Cuticle deposition ceases during strawberry fruit development. In: BMC plant biology. 2024 ; Vol. 24, No. 1.
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T1 - Cuticle deposition ceases during strawberry fruit development

AU - Straube, Jannis

AU - Hurtado, Grecia

AU - Zeisler-Diehl, Viktoria

AU - Schreiber, Lukas

AU - Knoche, Moritz

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024/6/29

Y1 - 2024/6/29

N2 - Background: Ideally, the barrier properties of a fruit’s cuticle persist throughout its development. This presents a challenge for strawberry fruit, with their rapid development and thin cuticles. The objective was to establish the developmental time course of cuticle deposition in strawberry fruit. Results: Fruit mass and surface area increase rapidly, with peak growth rate coinciding with the onset of ripening. On a whole-fruit basis, the masses of cutin and wax increase but on a unit surface-area basis, they decrease. The decrease is associated with marked increases in elastic strain. The expressions of cuticle-associated genes involved in transcriptional regulation (FaSHN1, FaSHN2, FaSHN3), synthesis of cutin (FaLACS2, FaGPAT3) and wax (FaCER1, FaKCS10, FaKCR1), and those involved in transport of cutin monomers and wax constituents (FaABCG11, FaABCG32) decreased until maturity. The only exceptions were FaLACS6 and FaGPAT6 that are presumably involved in cutin synthesis, and FaCER1 involved in wax synthesis. This result was consistent across five strawberry cultivars. Strawberry cutin consists mainly of C16 and C18 monomers, plus minor amounts of C19, C20, C22 and C24 monomers, ω-hydroxy acids, dihydroxy acids, epoxy acids, primary alcohols, carboxylic acids and dicarboxylic acids. The most abundant monomer is 10,16-dihydroxyhexadecanoic acid. Waxes comprise mainly long-chain fatty acids C29 to C46, with smaller amounts of C16 to C28. Wax constituents are carboxylic acids, primary alcohols, alkanes, aldehydes, sterols and esters. Conclusion: The downregulation of cuticle deposition during development accounts for the marked cuticular strain, for the associated microcracking, and for their high susceptibility to the disorders of water soaking and cracking.

AB - Background: Ideally, the barrier properties of a fruit’s cuticle persist throughout its development. This presents a challenge for strawberry fruit, with their rapid development and thin cuticles. The objective was to establish the developmental time course of cuticle deposition in strawberry fruit. Results: Fruit mass and surface area increase rapidly, with peak growth rate coinciding with the onset of ripening. On a whole-fruit basis, the masses of cutin and wax increase but on a unit surface-area basis, they decrease. The decrease is associated with marked increases in elastic strain. The expressions of cuticle-associated genes involved in transcriptional regulation (FaSHN1, FaSHN2, FaSHN3), synthesis of cutin (FaLACS2, FaGPAT3) and wax (FaCER1, FaKCS10, FaKCR1), and those involved in transport of cutin monomers and wax constituents (FaABCG11, FaABCG32) decreased until maturity. The only exceptions were FaLACS6 and FaGPAT6 that are presumably involved in cutin synthesis, and FaCER1 involved in wax synthesis. This result was consistent across five strawberry cultivars. Strawberry cutin consists mainly of C16 and C18 monomers, plus minor amounts of C19, C20, C22 and C24 monomers, ω-hydroxy acids, dihydroxy acids, epoxy acids, primary alcohols, carboxylic acids and dicarboxylic acids. The most abundant monomer is 10,16-dihydroxyhexadecanoic acid. Waxes comprise mainly long-chain fatty acids C29 to C46, with smaller amounts of C16 to C28. Wax constituents are carboxylic acids, primary alcohols, alkanes, aldehydes, sterols and esters. Conclusion: The downregulation of cuticle deposition during development accounts for the marked cuticular strain, for the associated microcracking, and for their high susceptibility to the disorders of water soaking and cracking.

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KW - Cutin

KW - Fragaria x ananassa Duch

KW - Strain

KW - Wax

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