Details
| Original language | English |
|---|---|
| Article number | 4290 |
| Pages (from-to) | 1-17 |
| Number of pages | 17 |
| Journal | MATERIALS |
| Volume | 13 |
| Issue number | 19 |
| Publication status | Published - 25 Sept 2020 |
Abstract
Due to the ever-increasing resolution of 3D printing technology, additive manufacturing is now even used to produce complex devices for laboratory applications. Personalized experimental devices or entire cultivation systems of almost unlimited complexity can potentially be manufactured within hours from start to finish—an enormous potential for experimental parallelization in a highly controllable environment. This study presents customized 3D-printed co-cultivation systems, which qualify for angiogenesis studies. In these systems, endothelial and mesenchymal stem cells (AD-MSC) were indirectly co-cultivated—that is, both cell types were physically separated through a rigid, 3D-printed barrier in the middle, while still sharing the same cell culture medium that allows for the exchange of signalling molecules. Biochemical-based cytotoxicity assays initially confirmed that the 3D printing material does not exert any negative effects on cells. Since the material also enables phase contrast and fluorescence microscopy, the behaviour of cells could be observed over the entire cultivation via both. Microscopic observations and subsequent quantitative analysis revealed that endothelial cells form tubular-like structures as angiogenic feature when indirectly co-cultured alongside AD-MSCs in the 3D-printed co-cultivation system. In addition, further 3D-printed devices are also introduced that address different issues and aspire to help in varying experimental setups. Our results mark an important step forward for the integration of customized 3D-printed systems as self-contained test systems or equipment in biomedical applications.
Keywords
- Additive manufacturing, Angiogenesis, Biomaterials, Biomedical application, Co-cultivation, Mammalian cell culture
ASJC Scopus subject areas
- Materials Science(all)
- General Materials Science
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In: MATERIALS, Vol. 13, No. 19, 4290, 25.09.2020, p. 1-17.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Customizable 3D-printed (Co-)cultivation systems for in vitro study of angiogenesis
AU - Siller, Ina G.
AU - Epping, Niklas Maximilian
AU - Lavrentieva, Antonina
AU - Scheper, Thomas
AU - Bahnemann, Janina
N1 - Funding Information: This research was funded by the German Research Foundation (DFG) via the Emmy Noether programme, project ID 346772917 and the publication of this article was funded by the Open Access fund of Leibniz Universit?t Hannover. The authors would like to thank Jan Ebbecke for his support in cell cultivation. Furthermore, we would like to thank Sebastian Heene and the group of Cornelia Blume (Institute of Technical Chemistry, Hannover, Germany), who kindly provided the endothelial cells. We acknowledge the corporation with Sarah Strauss and Peter Vogt, (Hannover Medical School, Hannover, Germany), who provided tissue material for AD-MSCs isolation. Finally, we would like to thank the group of Oliver Plettenburg (Institute of Organic Chemistry, Hannover, Germany) for providing the live cell imaging microscope.
PY - 2020/9/25
Y1 - 2020/9/25
N2 - Due to the ever-increasing resolution of 3D printing technology, additive manufacturing is now even used to produce complex devices for laboratory applications. Personalized experimental devices or entire cultivation systems of almost unlimited complexity can potentially be manufactured within hours from start to finish—an enormous potential for experimental parallelization in a highly controllable environment. This study presents customized 3D-printed co-cultivation systems, which qualify for angiogenesis studies. In these systems, endothelial and mesenchymal stem cells (AD-MSC) were indirectly co-cultivated—that is, both cell types were physically separated through a rigid, 3D-printed barrier in the middle, while still sharing the same cell culture medium that allows for the exchange of signalling molecules. Biochemical-based cytotoxicity assays initially confirmed that the 3D printing material does not exert any negative effects on cells. Since the material also enables phase contrast and fluorescence microscopy, the behaviour of cells could be observed over the entire cultivation via both. Microscopic observations and subsequent quantitative analysis revealed that endothelial cells form tubular-like structures as angiogenic feature when indirectly co-cultured alongside AD-MSCs in the 3D-printed co-cultivation system. In addition, further 3D-printed devices are also introduced that address different issues and aspire to help in varying experimental setups. Our results mark an important step forward for the integration of customized 3D-printed systems as self-contained test systems or equipment in biomedical applications.
AB - Due to the ever-increasing resolution of 3D printing technology, additive manufacturing is now even used to produce complex devices for laboratory applications. Personalized experimental devices or entire cultivation systems of almost unlimited complexity can potentially be manufactured within hours from start to finish—an enormous potential for experimental parallelization in a highly controllable environment. This study presents customized 3D-printed co-cultivation systems, which qualify for angiogenesis studies. In these systems, endothelial and mesenchymal stem cells (AD-MSC) were indirectly co-cultivated—that is, both cell types were physically separated through a rigid, 3D-printed barrier in the middle, while still sharing the same cell culture medium that allows for the exchange of signalling molecules. Biochemical-based cytotoxicity assays initially confirmed that the 3D printing material does not exert any negative effects on cells. Since the material also enables phase contrast and fluorescence microscopy, the behaviour of cells could be observed over the entire cultivation via both. Microscopic observations and subsequent quantitative analysis revealed that endothelial cells form tubular-like structures as angiogenic feature when indirectly co-cultured alongside AD-MSCs in the 3D-printed co-cultivation system. In addition, further 3D-printed devices are also introduced that address different issues and aspire to help in varying experimental setups. Our results mark an important step forward for the integration of customized 3D-printed systems as self-contained test systems or equipment in biomedical applications.
KW - Additive manufacturing
KW - Angiogenesis
KW - Biomaterials
KW - Biomedical application
KW - Co-cultivation
KW - Mammalian cell culture
UR - http://www.scopus.com/inward/record.url?scp=85093932826&partnerID=8YFLogxK
U2 - 10.3390/ma13194290
DO - 10.3390/ma13194290
M3 - Article
AN - SCOPUS:85093932826
VL - 13
SP - 1
EP - 17
JO - MATERIALS
JF - MATERIALS
SN - 1996-1944
IS - 19
M1 - 4290
ER -