Details
| Original language | English |
|---|---|
| Pages (from-to) | 440-444 |
| Number of pages | 5 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 23 |
| Issue number | 6 |
| Publication status | Published - Mar 1986 |
Abstract
Fluorometric measurements were performed in continuous aerobic cultures of Saccharomyces cerevisiae in order to study the effect of substrate concentration and residence time on the intracellular NADH-level. A modified Beyelermicrofluorometer probe (Beyeler et al. 1981) was used for the experiments. It was possible to use this sensor continuously up to five weeks without problems. The relative NADH-values obtained by the on-line monitoring of the NADH-dependent culture fluorescence were compared to the enzymatically determined NADH-content. Biomass estimation from fluorescence data was performed. During oxidative-reductive catabolism the deviation between calculated and measured data were below 5%. The differences between oxidative and oxidative-reductive catabolism were studied regarding glucose addition, dilution rate increase and aerobic-anaerobic transition. For synchronized continuous cultures, changes in dilution rate resulted in changes of the oscillating behaviour. Flow cytometric studies in comparison with fluorometric studies showed changes in budding behaviour during the oscillations.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Applied Microbiology and Biotechnology, Vol. 23, No. 6, 03.1986, p. 440-444.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Culture fluorescence studies on aerobic continuous cultures of Saccharomyces cerevisiae
AU - Scheper, Th
AU - Schügerl, K.
PY - 1986/3
Y1 - 1986/3
N2 - Fluorometric measurements were performed in continuous aerobic cultures of Saccharomyces cerevisiae in order to study the effect of substrate concentration and residence time on the intracellular NADH-level. A modified Beyelermicrofluorometer probe (Beyeler et al. 1981) was used for the experiments. It was possible to use this sensor continuously up to five weeks without problems. The relative NADH-values obtained by the on-line monitoring of the NADH-dependent culture fluorescence were compared to the enzymatically determined NADH-content. Biomass estimation from fluorescence data was performed. During oxidative-reductive catabolism the deviation between calculated and measured data were below 5%. The differences between oxidative and oxidative-reductive catabolism were studied regarding glucose addition, dilution rate increase and aerobic-anaerobic transition. For synchronized continuous cultures, changes in dilution rate resulted in changes of the oscillating behaviour. Flow cytometric studies in comparison with fluorometric studies showed changes in budding behaviour during the oscillations.
AB - Fluorometric measurements were performed in continuous aerobic cultures of Saccharomyces cerevisiae in order to study the effect of substrate concentration and residence time on the intracellular NADH-level. A modified Beyelermicrofluorometer probe (Beyeler et al. 1981) was used for the experiments. It was possible to use this sensor continuously up to five weeks without problems. The relative NADH-values obtained by the on-line monitoring of the NADH-dependent culture fluorescence were compared to the enzymatically determined NADH-content. Biomass estimation from fluorescence data was performed. During oxidative-reductive catabolism the deviation between calculated and measured data were below 5%. The differences between oxidative and oxidative-reductive catabolism were studied regarding glucose addition, dilution rate increase and aerobic-anaerobic transition. For synchronized continuous cultures, changes in dilution rate resulted in changes of the oscillating behaviour. Flow cytometric studies in comparison with fluorometric studies showed changes in budding behaviour during the oscillations.
UR - http://www.scopus.com/inward/record.url?scp=0022691505&partnerID=8YFLogxK
U2 - 10.1007/BF02346057
DO - 10.1007/BF02346057
M3 - Article
AN - SCOPUS:0022691505
VL - 23
SP - 440
EP - 444
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 6
ER -