Details
| Original language | English |
|---|---|
| Pages (from-to) | 370-376 |
| Number of pages | 7 |
| Journal | Journal of biotechnology |
| Volume | 167 |
| Issue number | 4 |
| Publication status | Published - 26 Jul 2013 |
Abstract
Technical scale (≥5l) cultivations of shear stress sensitive microorganisms are often difficult to perform, as common bioreactors are usually designed to maximize the oxygen input into the culture medium. This is achieved by mechanical stirrers, causing high shear stress. Examples for shear stress sensitive microorganisms, for which no specific cultivation systems exist, are many anaerobic bacteria and fungi, such as basidiomycetes. In this work a disposable bag bioreactor developed for cultivation of mammalian cells was investigated to evaluate its potential to cultivate shear stress sensitive anaerobic Eubacterium ramulus and shear stress sensitive basidiomycetes Flammulina velutipes and Pleurotus sapidus. All cultivations were compared with conventional stainless steel stirred tank reactors (STR) cultivations. Good growth of all investigated microorganisms cultivated in the bag reactor was found. E. ramulus showed growth rates of μ=0.56h-1 (bag) and μ=0.53h-1 (STR). Differences concerning morphology, enzymatic activities and growth in fungal cultivations were observed. In the bag reactor growth in form of small, independent pellets was observed while STR cultivations showed intense aggregation. F. velutipes reached higher biomass concentrations (21.2gl-1 DCW vs. 16.8gl-1 DCW) and up to 2-fold higher peptidolytic activities in comparison to cell cultivation in stirred tank reactors.
Keywords
- Anaerobic cultivation, Basidiomycetes, Eubacterium ramulus, Shear stress, Wave reactor
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of biotechnology, Vol. 167, No. 4, 26.07.2013, p. 370-376.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Cultivation of shear stress sensitive microorganisms in disposable bag reactor systems
AU - Jonczyk, Patrick
AU - Takenberg, Meike
AU - Hartwig, Steffen
AU - Beutel, Sascha
AU - Berger, Ralf G.
AU - Scheper, Thomas
PY - 2013/7/26
Y1 - 2013/7/26
N2 - Technical scale (≥5l) cultivations of shear stress sensitive microorganisms are often difficult to perform, as common bioreactors are usually designed to maximize the oxygen input into the culture medium. This is achieved by mechanical stirrers, causing high shear stress. Examples for shear stress sensitive microorganisms, for which no specific cultivation systems exist, are many anaerobic bacteria and fungi, such as basidiomycetes. In this work a disposable bag bioreactor developed for cultivation of mammalian cells was investigated to evaluate its potential to cultivate shear stress sensitive anaerobic Eubacterium ramulus and shear stress sensitive basidiomycetes Flammulina velutipes and Pleurotus sapidus. All cultivations were compared with conventional stainless steel stirred tank reactors (STR) cultivations. Good growth of all investigated microorganisms cultivated in the bag reactor was found. E. ramulus showed growth rates of μ=0.56h-1 (bag) and μ=0.53h-1 (STR). Differences concerning morphology, enzymatic activities and growth in fungal cultivations were observed. In the bag reactor growth in form of small, independent pellets was observed while STR cultivations showed intense aggregation. F. velutipes reached higher biomass concentrations (21.2gl-1 DCW vs. 16.8gl-1 DCW) and up to 2-fold higher peptidolytic activities in comparison to cell cultivation in stirred tank reactors.
AB - Technical scale (≥5l) cultivations of shear stress sensitive microorganisms are often difficult to perform, as common bioreactors are usually designed to maximize the oxygen input into the culture medium. This is achieved by mechanical stirrers, causing high shear stress. Examples for shear stress sensitive microorganisms, for which no specific cultivation systems exist, are many anaerobic bacteria and fungi, such as basidiomycetes. In this work a disposable bag bioreactor developed for cultivation of mammalian cells was investigated to evaluate its potential to cultivate shear stress sensitive anaerobic Eubacterium ramulus and shear stress sensitive basidiomycetes Flammulina velutipes and Pleurotus sapidus. All cultivations were compared with conventional stainless steel stirred tank reactors (STR) cultivations. Good growth of all investigated microorganisms cultivated in the bag reactor was found. E. ramulus showed growth rates of μ=0.56h-1 (bag) and μ=0.53h-1 (STR). Differences concerning morphology, enzymatic activities and growth in fungal cultivations were observed. In the bag reactor growth in form of small, independent pellets was observed while STR cultivations showed intense aggregation. F. velutipes reached higher biomass concentrations (21.2gl-1 DCW vs. 16.8gl-1 DCW) and up to 2-fold higher peptidolytic activities in comparison to cell cultivation in stirred tank reactors.
KW - Anaerobic cultivation
KW - Basidiomycetes
KW - Eubacterium ramulus
KW - Shear stress
KW - Wave reactor
UR - http://www.scopus.com/inward/record.url?scp=84883252567&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2013.07.018
DO - 10.1016/j.jbiotec.2013.07.018
M3 - Article
C2 - 23892193
AN - SCOPUS:84883252567
VL - 167
SP - 370
EP - 376
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 4
ER -