Details
| Original language | English |
|---|---|
| Article number | 541 |
| Journal | Fermentation |
| Volume | 8 |
| Issue number | 10 |
| Publication status | Published - 14 Oct 2022 |
Abstract
Hispidin (6-(3,4-dihydroxystyrl)-4-hydroxy-2-pyrone) production in submerged cultured mycelia of the basidiomycete Inonotus hispidus was doubled in shake flasks through irradiation with white light. The daily addition of 1 mM hydrogen peroxide as a chemical stressor and a repeated supplementation of the shake flask cultures with 2 mM caffeic acid, a biogenetic precursor, further increased the hispidin synthesis. These cultivation conditions were combined and applied to parallel fermentation trials on the 4 L scale using a classical stirred tank bioreactor and a wave bag bioreactor. No significant differences in biomass yield and colorant production were observed. The hispidin concentration in both bioreactors reached 5.5 g·L − 1, the highest ever published. Textile dyeing with hispidin was successful, but impeded by its limited light stability in comparison to industrial dyes. However, following the idea of sustainability and the flawless toxicity profile, applications in natural cosmetics, other daily implements, or even therapeutics appear promising.
Keywords
- Inonotus hispidus, bioprocess, elicitation, hispidin, induction, natural dye, precursor, stirred tank reactor, wave bag reactor
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Fermentation, Vol. 8, No. 10, 541, 14.10.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Cultivation of Inonotus hispidus in Stirred Tank and Wave Bag Bioreactors to Produce the Natural Colorant Hispidin
AU - Bergmann, Pia
AU - Takenberg, Meike
AU - Frank, Christina
AU - Zschätzsch, Marlen
AU - Werner, Anett
AU - Berger, Ralf G.
AU - Ersoy, Franziska
N1 - Funding Information: This research was funded by the German Federal Ministry of Education and Research, grant numbers 031B0879 and 031B1079A. The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover.
PY - 2022/10/14
Y1 - 2022/10/14
N2 - Hispidin (6-(3,4-dihydroxystyrl)-4-hydroxy-2-pyrone) production in submerged cultured mycelia of the basidiomycete Inonotus hispidus was doubled in shake flasks through irradiation with white light. The daily addition of 1 mM hydrogen peroxide as a chemical stressor and a repeated supplementation of the shake flask cultures with 2 mM caffeic acid, a biogenetic precursor, further increased the hispidin synthesis. These cultivation conditions were combined and applied to parallel fermentation trials on the 4 L scale using a classical stirred tank bioreactor and a wave bag bioreactor. No significant differences in biomass yield and colorant production were observed. The hispidin concentration in both bioreactors reached 5.5 g·L − 1, the highest ever published. Textile dyeing with hispidin was successful, but impeded by its limited light stability in comparison to industrial dyes. However, following the idea of sustainability and the flawless toxicity profile, applications in natural cosmetics, other daily implements, or even therapeutics appear promising.
AB - Hispidin (6-(3,4-dihydroxystyrl)-4-hydroxy-2-pyrone) production in submerged cultured mycelia of the basidiomycete Inonotus hispidus was doubled in shake flasks through irradiation with white light. The daily addition of 1 mM hydrogen peroxide as a chemical stressor and a repeated supplementation of the shake flask cultures with 2 mM caffeic acid, a biogenetic precursor, further increased the hispidin synthesis. These cultivation conditions were combined and applied to parallel fermentation trials on the 4 L scale using a classical stirred tank bioreactor and a wave bag bioreactor. No significant differences in biomass yield and colorant production were observed. The hispidin concentration in both bioreactors reached 5.5 g·L − 1, the highest ever published. Textile dyeing with hispidin was successful, but impeded by its limited light stability in comparison to industrial dyes. However, following the idea of sustainability and the flawless toxicity profile, applications in natural cosmetics, other daily implements, or even therapeutics appear promising.
KW - Inonotus hispidus
KW - bioprocess
KW - elicitation
KW - hispidin
KW - induction
KW - natural dye
KW - precursor
KW - stirred tank reactor
KW - wave bag reactor
UR - http://www.scopus.com/inward/record.url?scp=85140651294&partnerID=8YFLogxK
U2 - 10.3390/fermentation8100541
DO - 10.3390/fermentation8100541
M3 - Article
VL - 8
JO - Fermentation
JF - Fermentation
IS - 10
M1 - 541
ER -