Details
| Original language | English |
|---|---|
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | Plant cell reports |
| Volume | 23 |
| Issue number | 1-2 |
| Publication status | Published - 25 May 2004 |
Abstract
We have developed a simple protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum. Embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used for cryopreservation. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rates of 75%. An additional pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h also positively affected re-growth. Microscopic studies on viability revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died. Experiments in which the duration of the pre-culture period - i.e. the length of time the embryogenic suspension cells were exposed to 0.6 M sucrose - was varied showed that 2-4 days was the most optimal exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen callus.
Keywords
- Long-term storage, Pre-culture, Re-growth, Somatic embryogenesis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant cell reports, Vol. 23, No. 1-2, 25.05.2004, p. 1-8.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Cryopreservation of embryogenic suspension cultures of Cyclamen persicum Mill
AU - Winkelmann, Traud
AU - Mußmann, Viola
AU - Serek, Margrethe
PY - 2004/5/25
Y1 - 2004/5/25
N2 - We have developed a simple protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum. Embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used for cryopreservation. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rates of 75%. An additional pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h also positively affected re-growth. Microscopic studies on viability revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died. Experiments in which the duration of the pre-culture period - i.e. the length of time the embryogenic suspension cells were exposed to 0.6 M sucrose - was varied showed that 2-4 days was the most optimal exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen callus.
AB - We have developed a simple protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum. Embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used for cryopreservation. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rates of 75%. An additional pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h also positively affected re-growth. Microscopic studies on viability revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died. Experiments in which the duration of the pre-culture period - i.e. the length of time the embryogenic suspension cells were exposed to 0.6 M sucrose - was varied showed that 2-4 days was the most optimal exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen callus.
KW - Long-term storage
KW - Pre-culture
KW - Re-growth
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=4344659726&partnerID=8YFLogxK
U2 - 10.1007/s00299-004-0783-1
DO - 10.1007/s00299-004-0783-1
M3 - Article
C2 - 15160283
AN - SCOPUS:4344659726
VL - 23
SP - 1
EP - 8
JO - Plant cell reports
JF - Plant cell reports
SN - 0721-7714
IS - 1-2
ER -