Details
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding |
| Editors | Miklos Fari, Imre Holb, Gyorgy Bisztray |
| Pages | 391-396 |
| Number of pages | 6 |
| Publication status | Published - Nov 2006 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 725 I |
| ISSN (Print) | 0567-7572 |
Abstract
We have developed a protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum, which does not involve the need of sophisticated equipment. With the aim of achieving this simple protocol for cryopreservation, different cryoprotectants in the pre-culture and pre-treatment periods on cell growth and re-growth after cryopreservation were tested. For cryopreservation experiments embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rate of 75% based on the number of Petri dishes plated after thawing. Additionally, a pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h immediately prior to freezing, also positively affected re-growth. Experiments in which the optimal preculture duration was varied showed that 2-4 days was the best exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen control callus. Microscopic studies on viability using FDA (fluorescein diacetate) staining revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died.
Keywords
- Long-term storage, Ornamental plants, Pre-culture, Re-growth, Somatic embryogenesis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding. ed. / Miklos Fari; Imre Holb; Gyorgy Bisztray. 2006. p. 391-396 (Acta Horticulturae; Vol. 725 I).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Cryopreservation of embryogenic suspension cultures of Cyclamen persicum Mill.
AU - Mußmann, V.
AU - Serek, M.
AU - Winkelmann, T.
PY - 2006/11
Y1 - 2006/11
N2 - We have developed a protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum, which does not involve the need of sophisticated equipment. With the aim of achieving this simple protocol for cryopreservation, different cryoprotectants in the pre-culture and pre-treatment periods on cell growth and re-growth after cryopreservation were tested. For cryopreservation experiments embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rate of 75% based on the number of Petri dishes plated after thawing. Additionally, a pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h immediately prior to freezing, also positively affected re-growth. Experiments in which the optimal preculture duration was varied showed that 2-4 days was the best exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen control callus. Microscopic studies on viability using FDA (fluorescein diacetate) staining revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died.
AB - We have developed a protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum, which does not involve the need of sophisticated equipment. With the aim of achieving this simple protocol for cryopreservation, different cryoprotectants in the pre-culture and pre-treatment periods on cell growth and re-growth after cryopreservation were tested. For cryopreservation experiments embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rate of 75% based on the number of Petri dishes plated after thawing. Additionally, a pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h immediately prior to freezing, also positively affected re-growth. Experiments in which the optimal preculture duration was varied showed that 2-4 days was the best exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen control callus. Microscopic studies on viability using FDA (fluorescein diacetate) staining revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died.
KW - Long-term storage
KW - Ornamental plants
KW - Pre-culture
KW - Re-growth
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=33846487418&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:33846487418
SN - 906605719X
SN - 9789066057197
T3 - Acta Horticulturae
SP - 391
EP - 396
BT - Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding
A2 - Fari, Miklos
A2 - Holb, Imre
A2 - Bisztray, Gyorgy
ER -