Details
| Original language | English |
|---|---|
| Pages (from-to) | 113-119 |
| Number of pages | 7 |
| Journal | Bioresource technology |
| Volume | 171 |
| Publication status | Published - 22 Aug 2014 |
Abstract
Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast. ×. fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.
Keywords
- Basidiomycota, Biotransformation, Monokaryon, Pleurotus
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Environmental Science(all)
- Environmental Engineering
- Energy(all)
- Renewable Energy, Sustainability and the Environment
- Environmental Science(all)
- Waste Management and Disposal
Sustainable Development Goals
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In: Bioresource technology, Vol. 171, 22.08.2014, p. 113-119.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
AU - Omarini, Alejandra B.
AU - Plagemann, Ina
AU - Schimanski, Silke
AU - Krings, Ulrich
AU - Berger, Ralf G.
N1 - Funding information: A.O. was supported by DAAD Postdoctoral Fellowship and a young researchers stipendium from the Gottfried Wilhelm Leibniz Universität , Hannover.
PY - 2014/8/22
Y1 - 2014/8/22
N2 - Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast. ×. fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.
AB - Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast. ×. fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.
KW - Basidiomycota
KW - Biotransformation
KW - Monokaryon
KW - Pleurotus
UR - http://www.scopus.com/inward/record.url?scp=84906841696&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2014.08.061
DO - 10.1016/j.biortech.2014.08.061
M3 - Article
C2 - 25189516
AN - SCOPUS:84906841696
VL - 171
SP - 113
EP - 119
JO - Bioresource technology
JF - Bioresource technology
SN - 0960-8524
ER -