Details
| Original language | English |
|---|---|
| Pages (from-to) | 13615-13623 |
| Number of pages | 9 |
| Journal | Analytical chemistry |
| Volume | 93 |
| Issue number | 40 |
| Early online date | 30 Sept 2021 |
| Publication status | Published - 12 Oct 2021 |
Abstract
We introduce the coupling of droplet microfluidics and ion mobility spectrometry (IMS) to address the challenges of label-free and chemical-specific detection of compounds in individual droplets. In analogy to the established use of mass spectrometry, droplet-IMS coupling can be also achieved via electrospray ionization but with significantly less instrumental effort. Because IMS instruments do not require high-vacuum systems, they are very compact, cost-effective, and robust, making them an ideal candidate as a chemical-specific end-of-line detector for segmented flow experiments. Herein, we demonstrate the successful coupling of droplet microfluidics with a custom-built high-resolution drift tube IMS system for monitoring chemical reactions in nL-sized droplets in an oil phase. The analytes contained in each droplet were assigned according to their characteristic ion mobility with limit of detections down to 200 nM to 1 μM and droplet frequencies ranging from 0.1 to 0.5 Hz. Using a custom sheath flow electrospray interface, we have further achieved the chemical-specific monitoring of a biochemical transformation catalyzed by a few hundred yeast cells, at single droplet level.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Analytical chemistry, Vol. 93, No. 40, 12.10.2021, p. 13615-13623.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Coupling Droplet Microfluidics with Ion Mobility Spectrometry for Monitoring Chemical Conversions at Nanoliter Scale
AU - Hartner, Nora T.
AU - Wink, Konstantin
AU - Raddatz, Christian Robert
AU - Thoben, Christian
AU - Schirmer, Martin
AU - Zimmermann, Stefan
AU - Belder, Detlev
N1 - Funding Information: This work was funded by the Deutsche Forschungsgemeinschaft (DFG) Project numbers 396430937, 458829155, and FOR 2177. The authors also thank Carolina F. Costrino for her collaboration during the project.
PY - 2021/10/12
Y1 - 2021/10/12
N2 - We introduce the coupling of droplet microfluidics and ion mobility spectrometry (IMS) to address the challenges of label-free and chemical-specific detection of compounds in individual droplets. In analogy to the established use of mass spectrometry, droplet-IMS coupling can be also achieved via electrospray ionization but with significantly less instrumental effort. Because IMS instruments do not require high-vacuum systems, they are very compact, cost-effective, and robust, making them an ideal candidate as a chemical-specific end-of-line detector for segmented flow experiments. Herein, we demonstrate the successful coupling of droplet microfluidics with a custom-built high-resolution drift tube IMS system for monitoring chemical reactions in nL-sized droplets in an oil phase. The analytes contained in each droplet were assigned according to their characteristic ion mobility with limit of detections down to 200 nM to 1 μM and droplet frequencies ranging from 0.1 to 0.5 Hz. Using a custom sheath flow electrospray interface, we have further achieved the chemical-specific monitoring of a biochemical transformation catalyzed by a few hundred yeast cells, at single droplet level.
AB - We introduce the coupling of droplet microfluidics and ion mobility spectrometry (IMS) to address the challenges of label-free and chemical-specific detection of compounds in individual droplets. In analogy to the established use of mass spectrometry, droplet-IMS coupling can be also achieved via electrospray ionization but with significantly less instrumental effort. Because IMS instruments do not require high-vacuum systems, they are very compact, cost-effective, and robust, making them an ideal candidate as a chemical-specific end-of-line detector for segmented flow experiments. Herein, we demonstrate the successful coupling of droplet microfluidics with a custom-built high-resolution drift tube IMS system for monitoring chemical reactions in nL-sized droplets in an oil phase. The analytes contained in each droplet were assigned according to their characteristic ion mobility with limit of detections down to 200 nM to 1 μM and droplet frequencies ranging from 0.1 to 0.5 Hz. Using a custom sheath flow electrospray interface, we have further achieved the chemical-specific monitoring of a biochemical transformation catalyzed by a few hundred yeast cells, at single droplet level.
UR - http://www.scopus.com/inward/record.url?scp=85117188042&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.1c02883
DO - 10.1021/acs.analchem.1c02883
M3 - Article
AN - SCOPUS:85117188042
VL - 93
SP - 13615
EP - 13623
JO - Analytical chemistry
JF - Analytical chemistry
SN - 0003-2700
IS - 40
ER -