Details
| Original language | English |
|---|---|
| Pages (from-to) | 595-602 |
| Number of pages | 8 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 129 |
| Issue number | 5 |
| Early online date | 10 Dec 2019 |
| Publication status | Published - May 2020 |
Abstract
Polyhydroxyalkanoates (PHAs) present an eco-friendly alternative for conventional plastics. Industrial wastewater from the food industry is a copious source of organic carbon that can be recovered in the form of PHA. However, the wastewater composition varies considerably among the different industries demanding for an industry-specific investigation of the PHA production process. Wastewater from the yeast industry, besides its high concentration in organic carbon, also contains a high ammonium concentration which might decrease the PHA production. Thus, this study aims to investigate PHA production using yeast industry wastewater via an enriched mixed microbial culture (MMC). A less explored cultivation strategy, i.e., continuous cultivation for PHA production was evaluated and compared to the widely used batch cultivation. PHA accumulating MMC was enriched using a sequencing batch reactor (SBR) operated under aerobic dynamic feeding. An MMC dominated by the Thauera species was successfully enriched in the SBR. Experimental results showed that ammonium is indeed required for PHA accumulation and the complete absence of ammonium negatively affects the accumulation process. Using wastewater, batch and continuous feeding strategies, respectively, yielded the PHA accumulation of 72 % and 65 % per dry cell weight. Despite the slightly lower PHA accumulation with continuous cultivation, four times more biomass growth was produced. Consequently, higher theoretical PHA production (270 t/year) can be expected using continuous cultivation in half of the reactor volume (45 m3). Therefore, this study asserts the viability of continuous cultivation as a feasible investigatory tool and PHA production strategy.
Keywords
- Continuous cultivation, Mixed microbial culture, Polyhydroxyalkanoates, Theoretical polyhydroxyalkanoate production, Yeast industry wastewater
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of Bioscience and Bioengineering, Vol. 129, No. 5, 05.2020, p. 595-602.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Continuous cultivation strategy for yeast industrial wastewater-based polyhydroxyalkanoate production
AU - Bhalerao, Aniruddha
AU - Banerjee, Rintu
AU - Nogueira, Regina
N1 - Funding Information: We acknowledge the Deutscher Akademischer Austauschdienst (DAAD) model Indian Institute of Technology (IIT) Master Sandwich Program scholarships for Master students of IIT and Indian Institute of Management 2017/18 for a funding research stay of Mr. Bhalerao in the Institute for Sanitary Engineering and Waste Management (ISAH) (personal Ref. ID: 91674911). We would also like to acknowledge the technical support provided by Mr. B. Schwanda, Mrs. K. Kock and Dr. Ing. C. Lorey (ISAH) for the completion of this work.
PY - 2020/5
Y1 - 2020/5
N2 - Polyhydroxyalkanoates (PHAs) present an eco-friendly alternative for conventional plastics. Industrial wastewater from the food industry is a copious source of organic carbon that can be recovered in the form of PHA. However, the wastewater composition varies considerably among the different industries demanding for an industry-specific investigation of the PHA production process. Wastewater from the yeast industry, besides its high concentration in organic carbon, also contains a high ammonium concentration which might decrease the PHA production. Thus, this study aims to investigate PHA production using yeast industry wastewater via an enriched mixed microbial culture (MMC). A less explored cultivation strategy, i.e., continuous cultivation for PHA production was evaluated and compared to the widely used batch cultivation. PHA accumulating MMC was enriched using a sequencing batch reactor (SBR) operated under aerobic dynamic feeding. An MMC dominated by the Thauera species was successfully enriched in the SBR. Experimental results showed that ammonium is indeed required for PHA accumulation and the complete absence of ammonium negatively affects the accumulation process. Using wastewater, batch and continuous feeding strategies, respectively, yielded the PHA accumulation of 72 % and 65 % per dry cell weight. Despite the slightly lower PHA accumulation with continuous cultivation, four times more biomass growth was produced. Consequently, higher theoretical PHA production (270 t/year) can be expected using continuous cultivation in half of the reactor volume (45 m3). Therefore, this study asserts the viability of continuous cultivation as a feasible investigatory tool and PHA production strategy.
AB - Polyhydroxyalkanoates (PHAs) present an eco-friendly alternative for conventional plastics. Industrial wastewater from the food industry is a copious source of organic carbon that can be recovered in the form of PHA. However, the wastewater composition varies considerably among the different industries demanding for an industry-specific investigation of the PHA production process. Wastewater from the yeast industry, besides its high concentration in organic carbon, also contains a high ammonium concentration which might decrease the PHA production. Thus, this study aims to investigate PHA production using yeast industry wastewater via an enriched mixed microbial culture (MMC). A less explored cultivation strategy, i.e., continuous cultivation for PHA production was evaluated and compared to the widely used batch cultivation. PHA accumulating MMC was enriched using a sequencing batch reactor (SBR) operated under aerobic dynamic feeding. An MMC dominated by the Thauera species was successfully enriched in the SBR. Experimental results showed that ammonium is indeed required for PHA accumulation and the complete absence of ammonium negatively affects the accumulation process. Using wastewater, batch and continuous feeding strategies, respectively, yielded the PHA accumulation of 72 % and 65 % per dry cell weight. Despite the slightly lower PHA accumulation with continuous cultivation, four times more biomass growth was produced. Consequently, higher theoretical PHA production (270 t/year) can be expected using continuous cultivation in half of the reactor volume (45 m3). Therefore, this study asserts the viability of continuous cultivation as a feasible investigatory tool and PHA production strategy.
KW - Continuous cultivation
KW - Mixed microbial culture
KW - Polyhydroxyalkanoates
KW - Theoretical polyhydroxyalkanoate production
KW - Yeast industry wastewater
UR - http://www.scopus.com/inward/record.url?scp=85076247652&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2019.11.006
DO - 10.1016/j.jbiosc.2019.11.006
M3 - Article
C2 - 31836378
AN - SCOPUS:85076247652
VL - 129
SP - 595
EP - 602
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -