Details
| Original language | English |
|---|---|
| Article number | 2304 |
| Journal | Microorganisms |
| Volume | 10 |
| Issue number | 11 |
| Publication status | Published - 21 Nov 2022 |
Abstract
Microorganisms acting as sinks for the greenhouse gas nitrous oxide (N 2O) are gaining increasing attention in the development of strategies to control N 2O emissions. Non-denitrifying N 2O reducers are of particular interest because they can provide a real sink without contributing to N 2O release. The bacterial strain under investigation (IGB 4-14 T), isolated in a mesocosm experiment to study the litter decomposition of Phragmites australis (Cav.), is such an organism. It carries only a nos gene cluster with the sec-dependent Clade II nosZ and is able to consume significant amounts of N 2O under anoxic conditions. However, consumption activity is considerably affected by the O 2 level. The reduction of N 2O was not associated with cell growth, suggesting that no energy is conserved by anaerobic respiration. Therefore, the N 2O consumption of strain IGB 4-14 T rather serves as an electron sink for metabolism to sustain viability during transient anoxia and/or to detoxify high N 2O concentrations. Phylogenetic analysis of 16S rRNA gene similarity revealed that the strain belongs to the genus Flavobacterium. It shares a high similarity in the nos gene cluster composition and the amino acid similarity of the nosZ gene with various type strains of the genus. However, phylogenomic analysis and comparison of overall genome relatedness indices clearly demonstrated a novel species status of strain IGB 4-14 T, with Flavobacterium lacus being the most closely related species. Various phenotypic differences supported a demarcation from this species. Based on these results, we proposed a novel species Flavobacterium azooxidireducens sp. nov. (type strain IGB 4-14 T = LMG 29709 T = DSM 103580 T).
Keywords
- Clade II nosZ, Flavobacterium azooxidireducenssp. nov, nitrous oxide reduction, non-denitrifier, phylogenomic analysis
ASJC Scopus subject areas
- Medicine(all)
- Microbiology (medical)
- Immunology and Microbiology(all)
- Virology
- Immunology and Microbiology(all)
- Microbiology
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In: Microorganisms, Vol. 10, No. 11, 2304, 21.11.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Consumption of N2O by Flavobacterium azooxidireducens sp. nov. Isolated from Decomposing Leaf Litter of Phragmites australis (Cav.)
AU - Behrendt, Undine
AU - Spanner, Tobias
AU - Augustin, Jürgen
AU - Zak, Dominik H.
AU - Horn, Marcus A.
AU - Kolb, Steffen
AU - Ulrich, Andreas
N1 - Funding Information: The research was funded by the DFG, grant no. ZA 742/2-1, and the DFG Priority Program 1374 “Infrastructure-Biodiversity-Exploratories”, project no. 324639010. The publication of this article was supported by funds from the Leibniz Association’s Open Access Fund.
PY - 2022/11/21
Y1 - 2022/11/21
N2 - Microorganisms acting as sinks for the greenhouse gas nitrous oxide (N 2O) are gaining increasing attention in the development of strategies to control N 2O emissions. Non-denitrifying N 2O reducers are of particular interest because they can provide a real sink without contributing to N 2O release. The bacterial strain under investigation (IGB 4-14 T), isolated in a mesocosm experiment to study the litter decomposition of Phragmites australis (Cav.), is such an organism. It carries only a nos gene cluster with the sec-dependent Clade II nosZ and is able to consume significant amounts of N 2O under anoxic conditions. However, consumption activity is considerably affected by the O 2 level. The reduction of N 2O was not associated with cell growth, suggesting that no energy is conserved by anaerobic respiration. Therefore, the N 2O consumption of strain IGB 4-14 T rather serves as an electron sink for metabolism to sustain viability during transient anoxia and/or to detoxify high N 2O concentrations. Phylogenetic analysis of 16S rRNA gene similarity revealed that the strain belongs to the genus Flavobacterium. It shares a high similarity in the nos gene cluster composition and the amino acid similarity of the nosZ gene with various type strains of the genus. However, phylogenomic analysis and comparison of overall genome relatedness indices clearly demonstrated a novel species status of strain IGB 4-14 T, with Flavobacterium lacus being the most closely related species. Various phenotypic differences supported a demarcation from this species. Based on these results, we proposed a novel species Flavobacterium azooxidireducens sp. nov. (type strain IGB 4-14 T = LMG 29709 T = DSM 103580 T).
AB - Microorganisms acting as sinks for the greenhouse gas nitrous oxide (N 2O) are gaining increasing attention in the development of strategies to control N 2O emissions. Non-denitrifying N 2O reducers are of particular interest because they can provide a real sink without contributing to N 2O release. The bacterial strain under investigation (IGB 4-14 T), isolated in a mesocosm experiment to study the litter decomposition of Phragmites australis (Cav.), is such an organism. It carries only a nos gene cluster with the sec-dependent Clade II nosZ and is able to consume significant amounts of N 2O under anoxic conditions. However, consumption activity is considerably affected by the O 2 level. The reduction of N 2O was not associated with cell growth, suggesting that no energy is conserved by anaerobic respiration. Therefore, the N 2O consumption of strain IGB 4-14 T rather serves as an electron sink for metabolism to sustain viability during transient anoxia and/or to detoxify high N 2O concentrations. Phylogenetic analysis of 16S rRNA gene similarity revealed that the strain belongs to the genus Flavobacterium. It shares a high similarity in the nos gene cluster composition and the amino acid similarity of the nosZ gene with various type strains of the genus. However, phylogenomic analysis and comparison of overall genome relatedness indices clearly demonstrated a novel species status of strain IGB 4-14 T, with Flavobacterium lacus being the most closely related species. Various phenotypic differences supported a demarcation from this species. Based on these results, we proposed a novel species Flavobacterium azooxidireducens sp. nov. (type strain IGB 4-14 T = LMG 29709 T = DSM 103580 T).
KW - Clade II nosZ
KW - Flavobacterium azooxidireducenssp. nov
KW - nitrous oxide reduction
KW - non-denitrifier
KW - phylogenomic analysis
UR - http://www.scopus.com/inward/record.url?scp=85149627611&partnerID=8YFLogxK
U2 - 10.3390/microorganisms10112304
DO - 10.3390/microorganisms10112304
M3 - Article
VL - 10
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 11
M1 - 2304
ER -