Details
| Original language | English |
|---|---|
| Pages (from-to) | 1385-1389 |
| Number of pages | 5 |
| Journal | Archives of virology |
| Volume | 153 |
| Issue number | 7 |
| Publication status | Published - 10 Jun 2008 |
Abstract
An infectious full-length cDNA clone of potato virus M (PVM) was produced. Total RNA was extracted from PVM-infected Nicotiana hesperis plants and used for cDNA synthesis. Subsequent RT-PCR produced two DNA fragments of about 5.5 and 3.2 kbp, which were ligated downstream of an enhanced 35S cauliflower mosaic virus promoter. After cloning of the enhanced 35S promoter with the PVM sequence into a modified pBIN19 plasmid and electroporation of Agrobacterium tumefaciens, the agroinoculated PVM full-length clone (pPVM-flc) led to systemic PVM infections in different host plants, causing symptoms indistinguishable from those caused by wild-type PVM.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Virology
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In: Archives of virology, Vol. 153, No. 7, 10.06.2008, p. 1385-1389.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Construction of an infectious full-length cDNA clone of potato virus M
AU - Flatken, S.
AU - Ungewickell, V.
AU - Menzel, W.
AU - Maiss, E.
N1 - Funding Information: This study was supported by the Federal Ministry of Education and Research (Germany) within the project 0312632E.
PY - 2008/6/10
Y1 - 2008/6/10
N2 - An infectious full-length cDNA clone of potato virus M (PVM) was produced. Total RNA was extracted from PVM-infected Nicotiana hesperis plants and used for cDNA synthesis. Subsequent RT-PCR produced two DNA fragments of about 5.5 and 3.2 kbp, which were ligated downstream of an enhanced 35S cauliflower mosaic virus promoter. After cloning of the enhanced 35S promoter with the PVM sequence into a modified pBIN19 plasmid and electroporation of Agrobacterium tumefaciens, the agroinoculated PVM full-length clone (pPVM-flc) led to systemic PVM infections in different host plants, causing symptoms indistinguishable from those caused by wild-type PVM.
AB - An infectious full-length cDNA clone of potato virus M (PVM) was produced. Total RNA was extracted from PVM-infected Nicotiana hesperis plants and used for cDNA synthesis. Subsequent RT-PCR produced two DNA fragments of about 5.5 and 3.2 kbp, which were ligated downstream of an enhanced 35S cauliflower mosaic virus promoter. After cloning of the enhanced 35S promoter with the PVM sequence into a modified pBIN19 plasmid and electroporation of Agrobacterium tumefaciens, the agroinoculated PVM full-length clone (pPVM-flc) led to systemic PVM infections in different host plants, causing symptoms indistinguishable from those caused by wild-type PVM.
UR - http://www.scopus.com/inward/record.url?scp=45849146919&partnerID=8YFLogxK
U2 - 10.1007/s00705-008-0127-5
DO - 10.1007/s00705-008-0127-5
M3 - Article
C2 - 18543062
AN - SCOPUS:45849146919
VL - 153
SP - 1385
EP - 1389
JO - Archives of virology
JF - Archives of virology
SN - 0304-8608
IS - 7
ER -