Details
| Original language | English |
|---|---|
| Article number | 1949242 |
| Journal | Tissue barriers |
| Volume | 9 |
| Issue number | 4 |
| Early online date | 6 Jul 2021 |
| Publication status | Published - 2 Oct 2021 |
Abstract
Connexins (Cxs) form gap junction (GJ) channels linking vertebrate cells. During embryogenesis, Cxs are expressed as early as the 4-8 cell stage. As cells differentiate into pluripotent stem cells (PSCs) and during gastrulation, the Cx expression pattern is adapted. Knockdown of Cx43 and Cx45 does not interfere with embryogenic development until the blastula stage, questioning the role of Cxs in PSC physiology and development. Studies in cultivated and induced PSCs (iPSCs) showed that Cx43 is essential for the maintenance of self-renewal and the expression of pluripotency markers. It was found that the role of Cxs in PSCs is more related to regulation of transcription or cell-cell adherence than to formation of GJ channels. Furthermore, a crucial role of Cxs for the self-renewal and differentiation was shown in cultivated adult mesenchymal stem cells. This review aims to highlight aspects that link Cxs to the function and physiology of stem cell development.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Medicine(all)
- Histology
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Tissue barriers, Vol. 9, No. 4, 1949242, 02.10.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Connexins in the development and physiology of stem cells
AU - Ngezahayo, Anaclet
AU - Ruhe, Frederike A
N1 - Funding Information: This work was supported by the Federal Ministry of Education and Research (BMBF), Grant TRANS-LARA 02NUK051A and the Deutsche Forschungsgemeinschaft (DFG), Grant NG 4/10-1 The authors thank Philip Palarz for helping by producing the figure.
PY - 2021/10/2
Y1 - 2021/10/2
N2 - Connexins (Cxs) form gap junction (GJ) channels linking vertebrate cells. During embryogenesis, Cxs are expressed as early as the 4-8 cell stage. As cells differentiate into pluripotent stem cells (PSCs) and during gastrulation, the Cx expression pattern is adapted. Knockdown of Cx43 and Cx45 does not interfere with embryogenic development until the blastula stage, questioning the role of Cxs in PSC physiology and development. Studies in cultivated and induced PSCs (iPSCs) showed that Cx43 is essential for the maintenance of self-renewal and the expression of pluripotency markers. It was found that the role of Cxs in PSCs is more related to regulation of transcription or cell-cell adherence than to formation of GJ channels. Furthermore, a crucial role of Cxs for the self-renewal and differentiation was shown in cultivated adult mesenchymal stem cells. This review aims to highlight aspects that link Cxs to the function and physiology of stem cell development.
AB - Connexins (Cxs) form gap junction (GJ) channels linking vertebrate cells. During embryogenesis, Cxs are expressed as early as the 4-8 cell stage. As cells differentiate into pluripotent stem cells (PSCs) and during gastrulation, the Cx expression pattern is adapted. Knockdown of Cx43 and Cx45 does not interfere with embryogenic development until the blastula stage, questioning the role of Cxs in PSC physiology and development. Studies in cultivated and induced PSCs (iPSCs) showed that Cx43 is essential for the maintenance of self-renewal and the expression of pluripotency markers. It was found that the role of Cxs in PSCs is more related to regulation of transcription or cell-cell adherence than to formation of GJ channels. Furthermore, a crucial role of Cxs for the self-renewal and differentiation was shown in cultivated adult mesenchymal stem cells. This review aims to highlight aspects that link Cxs to the function and physiology of stem cell development.
UR - http://www.scopus.com/inward/record.url?scp=85109829857&partnerID=8YFLogxK
U2 - 10.1080/21688370.2021.1949242
DO - 10.1080/21688370.2021.1949242
M3 - Article
C2 - 34227910
VL - 9
JO - Tissue barriers
JF - Tissue barriers
SN - 2168-8362
IS - 4
M1 - 1949242
ER -