Details
| Original language | English |
|---|---|
| Pages (from-to) | 184-193 |
| Number of pages | 10 |
| Journal | Nature plants |
| Volume | 5 |
| Issue number | 2 |
| Early online date | 8 Feb 2019 |
| Publication status | Published - Feb 2019 |
Abstract
Eukaryotic mRNAs frequently contain upstream open reading frames (uORFs), encoding small peptides that may control translation of the main ORF (mORF). Here, we report the characterization of a distinct bicistronic transcript in Arabidopsis. We analysed loss-of-function phenotypes of the inorganic polyphosphatase TRIPHOSPHATE TUNNEL METALLOENZYME 3 (AtTTM3), and found that catalytically inactive versions of the enzyme could fully complement embryo and growth-related phenotypes. We could rationalize these puzzling findings by characterizing a uORF in the AtTTM3 locus encoding CELL DIVISION CYCLE PROTEIN 26 (CDC26), an orthologue of the cell cycle regulator. We demonstrate that AtCDC26 is part of the plant anaphase promoting complex/cyclosome (APC/C), regulates accumulation of APC/C target proteins and controls cell division, growth and embryo development. AtCDC26 and AtTTM3 are translated from a single transcript conserved across the plant lineage. While there is no apparent biochemical connection between the two gene products, AtTTM3 coordinates AtCDC26 translation by recruiting the transcript into polysomes. Our work highlights that uORFs may encode functional proteins in plant genomes.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Nature plants, Vol. 5, No. 2, 02.2019, p. 184-193.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Concerted expression of a cell cycle regulator and a metabolic enzyme from a bicistronic transcript in plants
AU - Lorenzo-Orts, Laura
AU - Witthoeft, Janika
AU - Deforges, Jules
AU - Martinez, Jacobo
AU - Loubéry, Sylvain
AU - Placzek, Aleksandra
AU - Poirier, Yves
AU - Hothorn, Ludwig A.
AU - Jaillais, Yvon
AU - Hothorn, Michael
N1 - Funding Information: The authors thank J. M. Perez-Perez for sending the CYCB1;1-GFP line, A. Wachter for lba1 and upf3-1 seeds, and R. Ulm, A. Wachter and N. Geldner for critical reading of the manuscript. This project was supported by an ERC starting grant from the European Research Council under the European Union’s Seventh Framework Programme (FP/2007–2013)/ERC Grant Agreement no. 310856, the Max Planck Society, the European Molecular Biology Organisation (EMBO) Young Investigator Programme (to M.H.), the Howard Hughes Medical Institute (International Research Scholar Award to M.H.) and the Swiss National Fund Sinergia grant (CRSII3-154471 to Y.P.).
PY - 2019/2
Y1 - 2019/2
N2 - Eukaryotic mRNAs frequently contain upstream open reading frames (uORFs), encoding small peptides that may control translation of the main ORF (mORF). Here, we report the characterization of a distinct bicistronic transcript in Arabidopsis. We analysed loss-of-function phenotypes of the inorganic polyphosphatase TRIPHOSPHATE TUNNEL METALLOENZYME 3 (AtTTM3), and found that catalytically inactive versions of the enzyme could fully complement embryo and growth-related phenotypes. We could rationalize these puzzling findings by characterizing a uORF in the AtTTM3 locus encoding CELL DIVISION CYCLE PROTEIN 26 (CDC26), an orthologue of the cell cycle regulator. We demonstrate that AtCDC26 is part of the plant anaphase promoting complex/cyclosome (APC/C), regulates accumulation of APC/C target proteins and controls cell division, growth and embryo development. AtCDC26 and AtTTM3 are translated from a single transcript conserved across the plant lineage. While there is no apparent biochemical connection between the two gene products, AtTTM3 coordinates AtCDC26 translation by recruiting the transcript into polysomes. Our work highlights that uORFs may encode functional proteins in plant genomes.
AB - Eukaryotic mRNAs frequently contain upstream open reading frames (uORFs), encoding small peptides that may control translation of the main ORF (mORF). Here, we report the characterization of a distinct bicistronic transcript in Arabidopsis. We analysed loss-of-function phenotypes of the inorganic polyphosphatase TRIPHOSPHATE TUNNEL METALLOENZYME 3 (AtTTM3), and found that catalytically inactive versions of the enzyme could fully complement embryo and growth-related phenotypes. We could rationalize these puzzling findings by characterizing a uORF in the AtTTM3 locus encoding CELL DIVISION CYCLE PROTEIN 26 (CDC26), an orthologue of the cell cycle regulator. We demonstrate that AtCDC26 is part of the plant anaphase promoting complex/cyclosome (APC/C), regulates accumulation of APC/C target proteins and controls cell division, growth and embryo development. AtCDC26 and AtTTM3 are translated from a single transcript conserved across the plant lineage. While there is no apparent biochemical connection between the two gene products, AtTTM3 coordinates AtCDC26 translation by recruiting the transcript into polysomes. Our work highlights that uORFs may encode functional proteins in plant genomes.
UR - http://www.scopus.com/inward/record.url?scp=85061296414&partnerID=8YFLogxK
U2 - 10.1038/s41477-019-0358-3
DO - 10.1038/s41477-019-0358-3
M3 - Article
C2 - 30737513
AN - SCOPUS:85061296414
VL - 5
SP - 184
EP - 193
JO - Nature plants
JF - Nature plants
SN - 2055-0278
IS - 2
ER -