TY - JOUR

T1 - Composition and Stability of the Oxidative Phosphorylation System in the Halophile Plant Cakile maritima

AU - Farhat, Nèjia

AU - Hichri, Sarra

AU - Hildebrandt, Tatjana Manuela

AU - Debez, Ahmed

AU - Braun, Hans Peter

N1 - Funding information: We thank Christa Ruppelt and Dagmar Lewejohann for the support in cell culture maintenance and mitochondria isolations. We also thank Dr. Holger Eubel for critically reading the manuscript. Funding. This study was supported by the German Academic Exchange Service (DAAD) in the framework of the “Change by Exchange”-program (program line 4), title of the project: “Proteomics and Halophyte Stress Tolerance” (ID 57247769). The publication of this manuscript was funded by the Open Access fund of Leibniz Universität Hannover.

PY - 2019/8/13

Y1 - 2019/8/13

N2 - Mitochondria play a central role in the energy metabolism of plants. At the same time, they provide energy for plant stress responses. We here report a first view on the mitochondrial Oxidative Phosphorylation (OXPHOS) system of the halophile (salt tolerant) plant Cakile maritima. Mitochondria were purified from suspension cultures of C. maritima and for comparison of Arabidopsis thaliana, a closely related glycophyte (salt sensitive) plant. Mitochondria were treated with digitonin and solubilized protein complexes were analyzed by 2D Blue native/SDS polyacrylamide gel electrophoresis. The OXPHOS systems of the two compared plants exhibit some distinct differences. C. maritima mitochondria include a very abundant respiratory supercomplex composed of monomeric complex I and dimeric complex III. At the same time the complexes II and IV are of reduced abundance. The stability of the OXPHOS complexes was investigated by combined salt and temperature treatments of isolated mitochondria. ATP synthase (complex V) is of increased stability in C. maritima. Also, the I + III2 supercomplex is present in high abundance during stress treatments. These results give insights into the mitochondrial contribution to the plant salt stress response.

AB - Mitochondria play a central role in the energy metabolism of plants. At the same time, they provide energy for plant stress responses. We here report a first view on the mitochondrial Oxidative Phosphorylation (OXPHOS) system of the halophile (salt tolerant) plant Cakile maritima. Mitochondria were purified from suspension cultures of C. maritima and for comparison of Arabidopsis thaliana, a closely related glycophyte (salt sensitive) plant. Mitochondria were treated with digitonin and solubilized protein complexes were analyzed by 2D Blue native/SDS polyacrylamide gel electrophoresis. The OXPHOS systems of the two compared plants exhibit some distinct differences. C. maritima mitochondria include a very abundant respiratory supercomplex composed of monomeric complex I and dimeric complex III. At the same time the complexes II and IV are of reduced abundance. The stability of the OXPHOS complexes was investigated by combined salt and temperature treatments of isolated mitochondria. ATP synthase (complex V) is of increased stability in C. maritima. Also, the I + III2 supercomplex is present in high abundance during stress treatments. These results give insights into the mitochondrial contribution to the plant salt stress response.

KW - Arabidopsis thaliana

KW - Blue native PAGE

KW - Cakile maritima

KW - halophyte

KW - mitochondria

KW - mitochondrial ATP synthase

KW - oxidative phosphorylation

KW - respiratory chain

UR - http://www.scopus.com/inward/record.url?scp=85072740119&partnerID=8YFLogxK

U2 - 10.3389/fpls.2019.01010

DO - 10.3389/fpls.2019.01010

M3 - Article

AN - SCOPUS:85072740119

VL - 10

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 1010

ER -