Details
| Original language | English |
|---|---|
| Pages (from-to) | 231-240 |
| Number of pages | 10 |
| Journal | VIRUS GENES |
| Volume | 36 |
| Issue number | 1 |
| Publication status | Published - 29 Nov 2007 |
Abstract
Okra mosaic virus (OkMV) is a tymovirus infecting members of the family Malvaceae. Early infections in okra (Abelmoschus esculentus) lead to yield losses of 12-19.5%. Besides intensive biological characterizations of OkMV only minor molecular data were available. Therefore, we determined the complete nucleotide sequence of a Nigerian isolate of OkMV. The complete genomic RNA (gRNA) comprises 6,223 nt and its genome organization showed three major ORFs coding for a putative movement protein (MP) of Mr 73.1 kDa, a large replication-associated protein (RP) of Mr 202.4 kDa and a coat protein (CP) of Mr 19.6 kDa. Prediction of secondary RNA structures showed three hairpin structures with internal loops in the 5′-untranslated region (UTR) and a 3′-terminal tRNA-like structure (TLS) which comprises the anticodon for valine, typical for a member of the genus Tymovirus. Phylogenetic comparisons based on the RP, MP and CP amino acid sequences showed the close relationship of OkMV not only to other completely sequenced tymoviruses like Kennedya yellow mosaic virus (KYMV), Turnip yellow mosaic virus (TYMV) and Erysimum latent virus (ErLV), but also to Calopogonium yellow vein virus (CalYVV), Clitoria yellow vein virus (CYVV) and Desmodium yellow mottle virus (DYMoV). This is the first report of a complete OkMV genome sequence from one of the various OkMV isolates originating from West Africa described so far. Additionally, the experimental host range of OkMV including several Nicotiana species was determined.
Keywords
- Complete sequence, Host range, Okra mosaic virus (OkMV), Phylogeny, Symptoms, Tymovirus
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Immunology and Microbiology(all)
- Virology
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In: VIRUS GENES, Vol. 36, No. 1, 29.11.2007, p. 231-240.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Complete nucleotide sequence and experimental host range of Okra mosaic virus
AU - Stephan, Dirk
AU - Siddiqua, Mahbuba
AU - Ta Hoang, Anh
AU - Engelmann, Jill
AU - Winter, Stephan
AU - Maiss, Edgar
PY - 2007/11/29
Y1 - 2007/11/29
N2 - Okra mosaic virus (OkMV) is a tymovirus infecting members of the family Malvaceae. Early infections in okra (Abelmoschus esculentus) lead to yield losses of 12-19.5%. Besides intensive biological characterizations of OkMV only minor molecular data were available. Therefore, we determined the complete nucleotide sequence of a Nigerian isolate of OkMV. The complete genomic RNA (gRNA) comprises 6,223 nt and its genome organization showed three major ORFs coding for a putative movement protein (MP) of Mr 73.1 kDa, a large replication-associated protein (RP) of Mr 202.4 kDa and a coat protein (CP) of Mr 19.6 kDa. Prediction of secondary RNA structures showed three hairpin structures with internal loops in the 5′-untranslated region (UTR) and a 3′-terminal tRNA-like structure (TLS) which comprises the anticodon for valine, typical for a member of the genus Tymovirus. Phylogenetic comparisons based on the RP, MP and CP amino acid sequences showed the close relationship of OkMV not only to other completely sequenced tymoviruses like Kennedya yellow mosaic virus (KYMV), Turnip yellow mosaic virus (TYMV) and Erysimum latent virus (ErLV), but also to Calopogonium yellow vein virus (CalYVV), Clitoria yellow vein virus (CYVV) and Desmodium yellow mottle virus (DYMoV). This is the first report of a complete OkMV genome sequence from one of the various OkMV isolates originating from West Africa described so far. Additionally, the experimental host range of OkMV including several Nicotiana species was determined.
AB - Okra mosaic virus (OkMV) is a tymovirus infecting members of the family Malvaceae. Early infections in okra (Abelmoschus esculentus) lead to yield losses of 12-19.5%. Besides intensive biological characterizations of OkMV only minor molecular data were available. Therefore, we determined the complete nucleotide sequence of a Nigerian isolate of OkMV. The complete genomic RNA (gRNA) comprises 6,223 nt and its genome organization showed three major ORFs coding for a putative movement protein (MP) of Mr 73.1 kDa, a large replication-associated protein (RP) of Mr 202.4 kDa and a coat protein (CP) of Mr 19.6 kDa. Prediction of secondary RNA structures showed three hairpin structures with internal loops in the 5′-untranslated region (UTR) and a 3′-terminal tRNA-like structure (TLS) which comprises the anticodon for valine, typical for a member of the genus Tymovirus. Phylogenetic comparisons based on the RP, MP and CP amino acid sequences showed the close relationship of OkMV not only to other completely sequenced tymoviruses like Kennedya yellow mosaic virus (KYMV), Turnip yellow mosaic virus (TYMV) and Erysimum latent virus (ErLV), but also to Calopogonium yellow vein virus (CalYVV), Clitoria yellow vein virus (CYVV) and Desmodium yellow mottle virus (DYMoV). This is the first report of a complete OkMV genome sequence from one of the various OkMV isolates originating from West Africa described so far. Additionally, the experimental host range of OkMV including several Nicotiana species was determined.
KW - Complete sequence
KW - Host range
KW - Okra mosaic virus (OkMV)
KW - Phylogeny
KW - Symptoms
KW - Tymovirus
UR - http://www.scopus.com/inward/record.url?scp=39049157446&partnerID=8YFLogxK
U2 - 10.1007/s11262-007-0181-1
DO - 10.1007/s11262-007-0181-1
M3 - Article
C2 - 18049886
AN - SCOPUS:39049157446
VL - 36
SP - 231
EP - 240
JO - VIRUS GENES
JF - VIRUS GENES
SN - 0920-8569
IS - 1
ER -