Details
| Original language | English |
|---|---|
| Article number | 001207 |
| Pages (from-to) | 308-320 |
| Number of pages | 13 |
| Journal | Journal of General Virology |
| Volume | 100 |
| Issue number | 2 |
| Early online date | 22 Jan 2019 |
| Publication status | E-pub ahead of print - 22 Jan 2019 |
Abstract
Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5¢ untranslated region (UTR) of 13 nt and a 3¢ UTR consisting of 586 nt that is not polyadenylated. CeLV RNA shares nt sequence identity of only about 40 % with other members of the Potyviridae (potyvirids). The CeLV polyprotein is notable in that it starts with a signal peptide, has a putative P3N-PIPO ORF and shares low aa sequence identity (about 18 %) with other potyvirids. Although potential cleavage sites were not identified for the N-terminal two-thirds of the polyprotein, the latter possesses a number of sequence motifs, the identity and position of which are characteristic of other potyvirids. Attempts at constructing an infectious full-length cDNA clone of CeLV were successful following Rhizobium radiobacter infiltration of Nicotiana benthamiana and Apium graveolens. CeLV appears to have the largest genome of all known potyvirids and some unique genome features that may warrant the creation of a new genus, for which we propose the name ‘celavirus’.
Keywords
- Celavirus, Celery latent virus, Full-length clone, Potyviridae, Signal peptide
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Virology
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In: Journal of General Virology, Vol. 100, No. 2, 001207, 22.01.2019, p. 308-320.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus – an unusual member of a putative new genus within the Potyviridae
AU - Rose, Hanna
AU - Döring, Ines
AU - Vetten, Heinrich Josef
AU - Menzel, Wulf
AU - Richert-Pöggeler, Katja R.
AU - Maiss, Edgar
PY - 2019/1/22
Y1 - 2019/1/22
N2 - Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5¢ untranslated region (UTR) of 13 nt and a 3¢ UTR consisting of 586 nt that is not polyadenylated. CeLV RNA shares nt sequence identity of only about 40 % with other members of the Potyviridae (potyvirids). The CeLV polyprotein is notable in that it starts with a signal peptide, has a putative P3N-PIPO ORF and shares low aa sequence identity (about 18 %) with other potyvirids. Although potential cleavage sites were not identified for the N-terminal two-thirds of the polyprotein, the latter possesses a number of sequence motifs, the identity and position of which are characteristic of other potyvirids. Attempts at constructing an infectious full-length cDNA clone of CeLV were successful following Rhizobium radiobacter infiltration of Nicotiana benthamiana and Apium graveolens. CeLV appears to have the largest genome of all known potyvirids and some unique genome features that may warrant the creation of a new genus, for which we propose the name ‘celavirus’.
AB - Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5¢ untranslated region (UTR) of 13 nt and a 3¢ UTR consisting of 586 nt that is not polyadenylated. CeLV RNA shares nt sequence identity of only about 40 % with other members of the Potyviridae (potyvirids). The CeLV polyprotein is notable in that it starts with a signal peptide, has a putative P3N-PIPO ORF and shares low aa sequence identity (about 18 %) with other potyvirids. Although potential cleavage sites were not identified for the N-terminal two-thirds of the polyprotein, the latter possesses a number of sequence motifs, the identity and position of which are characteristic of other potyvirids. Attempts at constructing an infectious full-length cDNA clone of CeLV were successful following Rhizobium radiobacter infiltration of Nicotiana benthamiana and Apium graveolens. CeLV appears to have the largest genome of all known potyvirids and some unique genome features that may warrant the creation of a new genus, for which we propose the name ‘celavirus’.
KW - Celavirus
KW - Celery latent virus
KW - Full-length clone
KW - Potyviridae
KW - Signal peptide
UR - http://www.scopus.com/inward/record.url?scp=85061118087&partnerID=8YFLogxK
U2 - 10.1099/jgv.0.001207
DO - 10.1099/jgv.0.001207
M3 - Article
C2 - 30667354
AN - SCOPUS:85061118087
VL - 100
SP - 308
EP - 320
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 2
M1 - 001207
ER -