Details
| Original language | English |
|---|---|
| Pages (from-to) | 3417-3420 |
| Number of pages | 4 |
| Journal | Archives of Virology |
| Volume | 166 |
| Issue number | 12 |
| Early online date | 4 Oct 2021 |
| Publication status | Published - Dec 2021 |
Abstract
Cucumber vein yellowing virus (CVYV) is a member of the genus Ipomovirus in the family Potyviridae. In the National Center for Biotechnology Information (NCBI) database, three complete genome sequences of CVYV isolates from Spain (NC_006941), Israel (KT276369), and Jordan (JF460793) are available. In this study, we report the complete sequence of an isolate of CVYV from Portugal (DSMZ PV-0776) along with the construction of an infectious full-length cDNA clone via Gibson assembly. The sequence of CVYV Portugal shows the closest relationship to a CVYV isolate from Spain (genome, 99.7% identity; polyprotein, 99.7% identity). The CVYV full-length cDNA clone was introduced by electroporation into Rhizobium radiobacter and infiltrated into the cotyledons of Cucumis sativus plantlets, resulting in symptoms resembling those of the wild-type virus. Transmission of the infectious CVYV full-length clone by the whitefly Bemisia tabaci was confirmed. This first report confirming the infectivity of a CVYV cDNA clone provides the opportunity to study gene functions in a consistent genomic background.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Virology
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In: Archives of Virology, Vol. 166, No. 12, 12.2021, p. 3417-3420.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Complete genome sequence and construction of an infectious full-length cDNA clone of a cucumber vein yellowing virus (CVYV) isolate from Portugal
AU - Cordes, Kaja
AU - Maiss, Edgar
AU - Winter, Stephan
AU - Rose, Hanna
N1 - Funding Information: We thank Birgit Milde for excellent technical assistance, and Dr. Wulf Menzel for providing CVYV-infected cucumber plantlets. Furthermore, we would like to thank Beate Stein for the Bemisia tabaci transmission.
PY - 2021/12
Y1 - 2021/12
N2 - Cucumber vein yellowing virus (CVYV) is a member of the genus Ipomovirus in the family Potyviridae. In the National Center for Biotechnology Information (NCBI) database, three complete genome sequences of CVYV isolates from Spain (NC_006941), Israel (KT276369), and Jordan (JF460793) are available. In this study, we report the complete sequence of an isolate of CVYV from Portugal (DSMZ PV-0776) along with the construction of an infectious full-length cDNA clone via Gibson assembly. The sequence of CVYV Portugal shows the closest relationship to a CVYV isolate from Spain (genome, 99.7% identity; polyprotein, 99.7% identity). The CVYV full-length cDNA clone was introduced by electroporation into Rhizobium radiobacter and infiltrated into the cotyledons of Cucumis sativus plantlets, resulting in symptoms resembling those of the wild-type virus. Transmission of the infectious CVYV full-length clone by the whitefly Bemisia tabaci was confirmed. This first report confirming the infectivity of a CVYV cDNA clone provides the opportunity to study gene functions in a consistent genomic background.
AB - Cucumber vein yellowing virus (CVYV) is a member of the genus Ipomovirus in the family Potyviridae. In the National Center for Biotechnology Information (NCBI) database, three complete genome sequences of CVYV isolates from Spain (NC_006941), Israel (KT276369), and Jordan (JF460793) are available. In this study, we report the complete sequence of an isolate of CVYV from Portugal (DSMZ PV-0776) along with the construction of an infectious full-length cDNA clone via Gibson assembly. The sequence of CVYV Portugal shows the closest relationship to a CVYV isolate from Spain (genome, 99.7% identity; polyprotein, 99.7% identity). The CVYV full-length cDNA clone was introduced by electroporation into Rhizobium radiobacter and infiltrated into the cotyledons of Cucumis sativus plantlets, resulting in symptoms resembling those of the wild-type virus. Transmission of the infectious CVYV full-length clone by the whitefly Bemisia tabaci was confirmed. This first report confirming the infectivity of a CVYV cDNA clone provides the opportunity to study gene functions in a consistent genomic background.
UR - http://www.scopus.com/inward/record.url?scp=85116310239&partnerID=8YFLogxK
U2 - 10.1007/s00705-021-05248-y
DO - 10.1007/s00705-021-05248-y
M3 - Article
VL - 166
SP - 3417
EP - 3420
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 12
ER -