Compatibility of Cells of the Nervous System with Structured Biodegradable Chitosan-Based Hydrogel Matrices

Research output: Contribution to journal › Article › Research › peer review

Authors

  • P. S. Timashev
  • K. N. Bardakova
  • N. V. Minaev
  • T. S. Demina
  • T. A. Mishchenko
  • E. V. Mitroshina
  • A. A. Akovantseva
  • A. V. Koroleva
  • D. S. Asyutin
  • L. F. Pimenova
  • N. A. Konovalov
  • T. A. Akopova
  • A. B. Solov’eva
  • I. V. Mukhina
  • M. V. Vedunova
  • B. N. Chichkov
  • V. N. Bagratashvili

External Research Organisations

  • Russian Academy of Sciences (RAS)
  • Nizhny Novgorod State Medical Acad.
  • Russian Ministry of Health
  • RAS - N.N.Semenov Institute of Chemical Physics
  • Nizhni Novgorod State University
View graph of relations

Details

Original languageEnglish
Pages (from-to)508-514
Number of pages7
JournalApplied Biochemistry and Microbiology
Volume52
Issue number5
Publication statusPublished - 10 Sept 2016
Externally publishedYes

Abstract

Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.

Keywords

    chitosan, neural networks, regenerative therapy, scaffolds, structured hydrogels, two-photon polymerization

ASJC Scopus subject areas

Cite this

Compatibility of Cells of the Nervous System with Structured Biodegradable Chitosan-Based Hydrogel Matrices. / Timashev, P. S.; Bardakova, K. N.; Minaev, N. V. et al.
In: Applied Biochemistry and Microbiology, Vol. 52, No. 5, 10.09.2016, p. 508-514.

Research output: Contribution to journal › Article › Research › peer review

Timashev, PS, Bardakova, KN, Minaev, NV, Demina, TS, Mishchenko, TA, Mitroshina, EV, Akovantseva, AA, Koroleva, AV, Asyutin, DS, Pimenova, LF, Konovalov, NA, Akopova, TA, Solov’eva, AB, Mukhina, IV, Vedunova, MV, Chichkov, BN & Bagratashvili, VN 2016, 'Compatibility of Cells of the Nervous System with Structured Biodegradable Chitosan-Based Hydrogel Matrices', Applied Biochemistry and Microbiology, vol. 52, no. 5, pp. 508-514. https://doi.org/10.1134/s0003683816050161
Timashev, P. S., Bardakova, K. N., Minaev, N. V., Demina, T. S., Mishchenko, T. A., Mitroshina, E. V., Akovantseva, A. A., Koroleva, A. V., Asyutin, D. S., Pimenova, L. F., Konovalov, N. A., Akopova, T. A., Solov’eva, A. B., Mukhina, I. V., Vedunova, M. V., Chichkov, B. N., & Bagratashvili, V. N. (2016). Compatibility of Cells of the Nervous System with Structured Biodegradable Chitosan-Based Hydrogel Matrices. Applied Biochemistry and Microbiology, 52(5), 508-514. https://doi.org/10.1134/s0003683816050161
Timashev PS, Bardakova KN, Minaev NV, Demina TS, Mishchenko TA, Mitroshina EV et al. Compatibility of Cells of the Nervous System with Structured Biodegradable Chitosan-Based Hydrogel Matrices. Applied Biochemistry and Microbiology. 2016 Sept 10;52(5):508-514. doi: 10.1134/s0003683816050161
Timashev, P. S. ; Bardakova, K. N. ; Minaev, N. V. et al. / Compatibility of Cells of the Nervous System with Structured Biodegradable Chitosan-Based Hydrogel Matrices. In: Applied Biochemistry and Microbiology. 2016 ; Vol. 52, No. 5. pp. 508-514.
Download
@article{7f756b93ed32491d95af6096c0a5366c,
title = "Compatibility of Cells of the Nervous System with Structured Biodegradable Chitosan-Based Hydrogel Matrices",
abstract = "Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.",
keywords = "chitosan, neural networks, regenerative therapy, scaffolds, structured hydrogels, two-photon polymerization",
author = "Timashev, {P. S.} and Bardakova, {K. N.} and Minaev, {N. V.} and Demina, {T. S.} and Mishchenko, {T. A.} and Mitroshina, {E. V.} and Akovantseva, {A. A.} and Koroleva, {A. V.} and Asyutin, {D. S.} and Pimenova, {L. F.} and Konovalov, {N. A.} and Akopova, {T. A.} and Solov{\textquoteright}eva, {A. B.} and Mukhina, {I. V.} and Vedunova, {M. V.} and Chichkov, {B. N.} and Bagratashvili, {V. N.}",
year = "2016",
month = sep,
day = "10",
doi = "10.1134/s0003683816050161",
language = "English",
volume = "52",
pages = "508--514",
journal = "Applied Biochemistry and Microbiology",
issn = "0003-6838",
publisher = "Pleiades Publishing",
number = "5",

}

Download

TY - JOUR

T1 - Compatibility of Cells of the Nervous System with Structured Biodegradable Chitosan-Based Hydrogel Matrices

AU - Timashev, P. S.

AU - Bardakova, K. N.

AU - Minaev, N. V.

AU - Demina, T. S.

AU - Mishchenko, T. A.

AU - Mitroshina, E. V.

AU - Akovantseva, A. A.

AU - Koroleva, A. V.

AU - Asyutin, D. S.

AU - Pimenova, L. F.

AU - Konovalov, N. A.

AU - Akopova, T. A.

AU - Solov’eva, A. B.

AU - Mukhina, I. V.

AU - Vedunova, M. V.

AU - Chichkov, B. N.

AU - Bagratashvili, V. N.

PY - 2016/9/10

Y1 - 2016/9/10

N2 - Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.

AB - Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.

KW - chitosan

KW - neural networks

KW - regenerative therapy

KW - scaffolds

KW - structured hydrogels

KW - two-photon polymerization

UR - http://www.scopus.com/inward/record.url?scp=84987654691&partnerID=8YFLogxK

U2 - 10.1134/s0003683816050161

DO - 10.1134/s0003683816050161

M3 - Article

AN - SCOPUS:84987654691

VL - 52

SP - 508

EP - 514

JO - Applied Biochemistry and Microbiology

JF - Applied Biochemistry and Microbiology

SN - 0003-6838

IS - 5

ER -