Details
| Original language | English |
|---|---|
| Pages (from-to) | 222-229 |
| Number of pages | 8 |
| Journal | Journal of biotechnology |
| Volume | 154 |
| Issue number | 4 |
| Publication status | Published - 22 Apr 2011 |
Abstract
The polysialic acid (PSA) production in Escherichia coli (E. coli) K1 was studied using three different cultivation strategies. A batch cultivation, a fed-batch cultivation at a constant specific growth rate of 0.25h -1 and a fed-batch cultivation at a constant glucose concentration of 50mgl -1 was performed. PSA formation kinetics under different cultivation strategies were analyzed based on the Monod growth model and the Luedeking-Piret equation. The results revealed that PSA formation in E. coli K1 was completely growth associated, the highest specific PSA formation rate (0.0489gg -1h -1) was obtained in the batch cultivation. However, comparing biomass and PSA yields on the glucose consumed, both fed-batch cultivations provided higher yields than that of the batch cultivation and acetate formation was prevented. Moreover, PSA yield on glucose was also correlated to the specific growth rate of the cells. The optimal specific growth rate for PSA production was 0.32h -1 obtained in the fed-batch cultivation at a constant glucose concentration of 50mgl -1, with highest conversion efficiency of 43mgg -1.
Keywords
- Escherichia coli k1, Fed-batch cultivation, Flow injection analysis, Kinetic modeling, Polysialic acid
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 154, No. 4, 22.04.2011, p. 222-229.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Comparison of polysialic acid production in Escherichia coli K1 during batch cultivation and fed-batch cultivation applying two different control strategies
AU - Chen, Ran
AU - John, Jinu
AU - Rode, Bastian
AU - Hitzmann, Bernd
AU - Gerardy-Schahn, Rita
AU - Kasper, Cornelia
AU - Scheper, Thomas
PY - 2011/4/22
Y1 - 2011/4/22
N2 - The polysialic acid (PSA) production in Escherichia coli (E. coli) K1 was studied using three different cultivation strategies. A batch cultivation, a fed-batch cultivation at a constant specific growth rate of 0.25h -1 and a fed-batch cultivation at a constant glucose concentration of 50mgl -1 was performed. PSA formation kinetics under different cultivation strategies were analyzed based on the Monod growth model and the Luedeking-Piret equation. The results revealed that PSA formation in E. coli K1 was completely growth associated, the highest specific PSA formation rate (0.0489gg -1h -1) was obtained in the batch cultivation. However, comparing biomass and PSA yields on the glucose consumed, both fed-batch cultivations provided higher yields than that of the batch cultivation and acetate formation was prevented. Moreover, PSA yield on glucose was also correlated to the specific growth rate of the cells. The optimal specific growth rate for PSA production was 0.32h -1 obtained in the fed-batch cultivation at a constant glucose concentration of 50mgl -1, with highest conversion efficiency of 43mgg -1.
AB - The polysialic acid (PSA) production in Escherichia coli (E. coli) K1 was studied using three different cultivation strategies. A batch cultivation, a fed-batch cultivation at a constant specific growth rate of 0.25h -1 and a fed-batch cultivation at a constant glucose concentration of 50mgl -1 was performed. PSA formation kinetics under different cultivation strategies were analyzed based on the Monod growth model and the Luedeking-Piret equation. The results revealed that PSA formation in E. coli K1 was completely growth associated, the highest specific PSA formation rate (0.0489gg -1h -1) was obtained in the batch cultivation. However, comparing biomass and PSA yields on the glucose consumed, both fed-batch cultivations provided higher yields than that of the batch cultivation and acetate formation was prevented. Moreover, PSA yield on glucose was also correlated to the specific growth rate of the cells. The optimal specific growth rate for PSA production was 0.32h -1 obtained in the fed-batch cultivation at a constant glucose concentration of 50mgl -1, with highest conversion efficiency of 43mgg -1.
KW - Escherichia coli k1
KW - Fed-batch cultivation
KW - Flow injection analysis
KW - Kinetic modeling
KW - Polysialic acid
UR - http://www.scopus.com/inward/record.url?scp=79960393946&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2011.04.009
DO - 10.1016/j.jbiotec.2011.04.009
M3 - Article
C2 - 21530596
AN - SCOPUS:79960393946
VL - 154
SP - 222
EP - 229
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 4
ER -