Details
| Original language | English |
|---|---|
| Pages (from-to) | 165-168 |
| Number of pages | 4 |
| Journal | Meat science |
| Volume | 59 |
| Issue number | 2 |
| Early online date | 11 Jul 2001 |
| Publication status | Published - Oct 2001 |
Abstract
A rinsing-technique with a spraying gun in combination with the ATP-bioluminescence for determining the microbial load was investigated. In each case 3.8-cm2 pig-rind was rinsed with buffered peptone water using a modified sprinkle-bottle. The sample-suspensions were gathered in a one-way syringe and sent through a filter cascade consisting of a coarse filter in order to remove bigger particles and a sensitive filter to attach the micro-organisms as well as somatic cells. This procedure was followed by an extraction of the ATP from the sensitive filter with CellSolver FBC-solution. The destructively gained samples were determined on their TVC by the conventional method. Results of the carcass-surface spray test combined with bioluminescence compared to the conventional destructive method followed by plate-count showed a correlation r=0.93 with a detection limit down to 104 cfu/cm2.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
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In: Meat science, Vol. 59, No. 2, 10.2001, p. 165-168.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Comparison of destructively and rinsing gained samples to determine TVC of pig carcasses by bioluminescence
AU - Werlein, H. D.
PY - 2001/10
Y1 - 2001/10
N2 - A rinsing-technique with a spraying gun in combination with the ATP-bioluminescence for determining the microbial load was investigated. In each case 3.8-cm2 pig-rind was rinsed with buffered peptone water using a modified sprinkle-bottle. The sample-suspensions were gathered in a one-way syringe and sent through a filter cascade consisting of a coarse filter in order to remove bigger particles and a sensitive filter to attach the micro-organisms as well as somatic cells. This procedure was followed by an extraction of the ATP from the sensitive filter with CellSolver FBC-solution. The destructively gained samples were determined on their TVC by the conventional method. Results of the carcass-surface spray test combined with bioluminescence compared to the conventional destructive method followed by plate-count showed a correlation r=0.93 with a detection limit down to 104 cfu/cm2.
AB - A rinsing-technique with a spraying gun in combination with the ATP-bioluminescence for determining the microbial load was investigated. In each case 3.8-cm2 pig-rind was rinsed with buffered peptone water using a modified sprinkle-bottle. The sample-suspensions were gathered in a one-way syringe and sent through a filter cascade consisting of a coarse filter in order to remove bigger particles and a sensitive filter to attach the micro-organisms as well as somatic cells. This procedure was followed by an extraction of the ATP from the sensitive filter with CellSolver FBC-solution. The destructively gained samples were determined on their TVC by the conventional method. Results of the carcass-surface spray test combined with bioluminescence compared to the conventional destructive method followed by plate-count showed a correlation r=0.93 with a detection limit down to 104 cfu/cm2.
UR - http://www.scopus.com/inward/record.url?scp=0035645232&partnerID=8YFLogxK
U2 - 10.1016/S0309-1740(01)00066-3
DO - 10.1016/S0309-1740(01)00066-3
M3 - Article
AN - SCOPUS:0035645232
VL - 59
SP - 165
EP - 168
JO - Meat science
JF - Meat science
SN - 0309-1740
IS - 2
ER -