Details
| Original language | English |
|---|---|
| Pages (from-to) | 35-37 |
| Number of pages | 3 |
| Journal | Analytical biochemistry |
| Volume | 477 |
| Early online date | 12 Feb 2015 |
| Publication status | Published - 15 May 2015 |
Abstract
In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming component above 1 and 5 wt% had considerable effects on the protein quantification in both assays, respectively, resulting in diminished protein recoveries/absorption values by increasing poly(ethylene glycol) (PEG)/salt concentration and PEG molecular weight. Therefore, a convenient dilution of both components (up to 1 and 5 wt%) before protein quantification is recommended in both assays, respectively, where the BCA assay is favored in comparison with the Bradford assay.
Keywords
- Aqueous two-phase system, BCA assay, Bradford assay, Colorimetric analysis, Protein quantification
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Analytical biochemistry, Vol. 477, 15.05.2015, p. 35-37.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems
AU - Glyk, Anna
AU - Heinisch, Sandra L.
AU - Scheper, Thomas
AU - Beutel, Sascha
PY - 2015/5/15
Y1 - 2015/5/15
N2 - In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming component above 1 and 5 wt% had considerable effects on the protein quantification in both assays, respectively, resulting in diminished protein recoveries/absorption values by increasing poly(ethylene glycol) (PEG)/salt concentration and PEG molecular weight. Therefore, a convenient dilution of both components (up to 1 and 5 wt%) before protein quantification is recommended in both assays, respectively, where the BCA assay is favored in comparison with the Bradford assay.
AB - In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming component above 1 and 5 wt% had considerable effects on the protein quantification in both assays, respectively, resulting in diminished protein recoveries/absorption values by increasing poly(ethylene glycol) (PEG)/salt concentration and PEG molecular weight. Therefore, a convenient dilution of both components (up to 1 and 5 wt%) before protein quantification is recommended in both assays, respectively, where the BCA assay is favored in comparison with the Bradford assay.
KW - Aqueous two-phase system
KW - BCA assay
KW - Bradford assay
KW - Colorimetric analysis
KW - Protein quantification
UR - http://www.scopus.com/inward/record.url?scp=84963937436&partnerID=8YFLogxK
U2 - 10.15488/1324
DO - 10.15488/1324
M3 - Article
C2 - 25684109
AN - SCOPUS:84963937436
VL - 477
SP - 35
EP - 37
JO - Analytical biochemistry
JF - Analytical biochemistry
SN - 0003-2697
ER -