Details
| Original language | English |
|---|---|
| Pages (from-to) | 259-267 |
| Number of pages | 9 |
| Journal | Proceedings of the Pakistan Academy of Sciences |
| Volume | 49 |
| Issue number | 4 |
| Publication status | Published - Dec 2012 |
Abstract
Rdrl is the first monogenic dominant resistance gene discovered in genus rosa. This locus is active against a fungal disease, black spot, of roses. Rdrl locus consists of nine genes named as muRdrlA to muRdrll. Functional and molecular characterization of this locus, revealed the presence of one gene, muRdrlH, active against Dort E4, race 6 of Diplocarpon rosae. In the present study we aimed to analyze the protein sequences of three members of this Rdrl gene family for their homologies, important domains, conserved regions and selection pressure to get insights of their functionality based on protein sequence. The protein sequences of one active gene, muRdrlH and two inactive genes, mu Rdr 1C and muRdrlG, were deduced and dissected using internet based bioinformatics tools. All three proteins belong to the T1R-NBS-LRR family of resistance genes. Although these proteins carry all necessary domains and conserved regions necessary for the functionality ofTIR-NBS-LRR proteins, LRR region of the proteins, found under selection pressure, indicated its role in effector identification.
Keywords
- Black spot, Rdr1, Resistance genes, Rose, TIR-NBS-LRR proteins
ASJC Scopus subject areas
- Social Sciences(all)
- General Social Sciences
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In: Proceedings of the Pakistan Academy of Sciences, Vol. 49, No. 4, 12.2012, p. 259-267.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Comparative sequence analysis of some Rdrl resistance genes from Rosa multiflora
AU - Yasmin, Aneela
AU - Jalbani, Akhtar A.
AU - Debener, Thomas
PY - 2012/12
Y1 - 2012/12
N2 - Rdrl is the first monogenic dominant resistance gene discovered in genus rosa. This locus is active against a fungal disease, black spot, of roses. Rdrl locus consists of nine genes named as muRdrlA to muRdrll. Functional and molecular characterization of this locus, revealed the presence of one gene, muRdrlH, active against Dort E4, race 6 of Diplocarpon rosae. In the present study we aimed to analyze the protein sequences of three members of this Rdrl gene family for their homologies, important domains, conserved regions and selection pressure to get insights of their functionality based on protein sequence. The protein sequences of one active gene, muRdrlH and two inactive genes, mu Rdr 1C and muRdrlG, were deduced and dissected using internet based bioinformatics tools. All three proteins belong to the T1R-NBS-LRR family of resistance genes. Although these proteins carry all necessary domains and conserved regions necessary for the functionality ofTIR-NBS-LRR proteins, LRR region of the proteins, found under selection pressure, indicated its role in effector identification.
AB - Rdrl is the first monogenic dominant resistance gene discovered in genus rosa. This locus is active against a fungal disease, black spot, of roses. Rdrl locus consists of nine genes named as muRdrlA to muRdrll. Functional and molecular characterization of this locus, revealed the presence of one gene, muRdrlH, active against Dort E4, race 6 of Diplocarpon rosae. In the present study we aimed to analyze the protein sequences of three members of this Rdrl gene family for their homologies, important domains, conserved regions and selection pressure to get insights of their functionality based on protein sequence. The protein sequences of one active gene, muRdrlH and two inactive genes, mu Rdr 1C and muRdrlG, were deduced and dissected using internet based bioinformatics tools. All three proteins belong to the T1R-NBS-LRR family of resistance genes. Although these proteins carry all necessary domains and conserved regions necessary for the functionality ofTIR-NBS-LRR proteins, LRR region of the proteins, found under selection pressure, indicated its role in effector identification.
KW - Black spot
KW - Rdr1
KW - Resistance genes
KW - Rose
KW - TIR-NBS-LRR proteins
UR - http://www.scopus.com/inward/record.url?scp=84872979354&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84872979354
VL - 49
SP - 259
EP - 267
JO - Proceedings of the Pakistan Academy of Sciences
JF - Proceedings of the Pakistan Academy of Sciences
SN - 0377-2969
IS - 4
ER -