Details
| Original language | English |
|---|---|
| Pages (from-to) | 123-133 |
| Number of pages | 11 |
| Journal | Journal of proteomics |
| Volume | 78 |
| Publication status | Published - 22 Nov 2012 |
Abstract
Somatic embryogenesis can efficiently foster the propagation of Theobroma cacao, but the poor quality of resulted plantlet hinders the use of this technique in the commercial scale. The current study has been initiated to systematically compare the physiological mechanisms underlying somatic and zygotic embryogenesis in T. cacao on the proteome level. About 1000 protein spots per fraction could be separated by two-dimensional isoelectric focusing/SDS PAGE. More than 50 of the protein spots clearly differed in abundance between zygotic and somatic embryos: 33 proteins spots were at least 3-fold higher in abundance in zygotic embryos and 20 in somatic embryos. Analyses of these protein spots differing in volume by mass spectrometry resulted in the identification of 68 distinct proteins. Many of the identified proteins are involved in genetic information processing (21 proteins), carbohydrate metabolism (11 proteins) and stress response (7 proteins). Somatic embryos especially displayed many stress related proteins, few enzymes involved in storage compound synthesis and an exceptional high abundance of endopeptidase inhibitors. Phosphoenolpyruvate carboxylase, which was accumulated more than 3-fold higher in zygotic embryos, represents a prominent enzyme in the storage compound metabolism in cacao seeds. Implications on the improvement of somatic embryogenesis in cacao are discussed.
Keywords
- Embryogenesis, Metabolic pathways, Proteomics, Theobroma cacao
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
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In: Journal of proteomics, Vol. 78, 22.11.2012, p. 123-133.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Comparative proteomic analysis of early somatic and zygotic embryogenesis in Theobroma cacao L.
AU - Noah, Alexandre Mboene
AU - Niemenak, Nicolas
AU - Sunderhaus, Stephanie
AU - Haase, Christin
AU - Omokolo, Denis Ndoumou
AU - Winkelmann, Traud
AU - Braun, Hans Peter
N1 - Funding information: This research has been carried out at the Institute of Plant Genetics (Department of Plant Proteomics) of Hannover University, Germany. Work of Alexandre Mboene Noah was financed by a short-term Wood–Whelan Fellowship from the International Union of Biochemistry and Molecular Biology (IUBMB) and by the Institute of Plant Genetics . Support of Alexandre Mboene Noah during his stay in Germany by Kerstin Hartung and Dr. Wolfgang Geller is gratefully acknowledged.
PY - 2012/11/22
Y1 - 2012/11/22
N2 - Somatic embryogenesis can efficiently foster the propagation of Theobroma cacao, but the poor quality of resulted plantlet hinders the use of this technique in the commercial scale. The current study has been initiated to systematically compare the physiological mechanisms underlying somatic and zygotic embryogenesis in T. cacao on the proteome level. About 1000 protein spots per fraction could be separated by two-dimensional isoelectric focusing/SDS PAGE. More than 50 of the protein spots clearly differed in abundance between zygotic and somatic embryos: 33 proteins spots were at least 3-fold higher in abundance in zygotic embryos and 20 in somatic embryos. Analyses of these protein spots differing in volume by mass spectrometry resulted in the identification of 68 distinct proteins. Many of the identified proteins are involved in genetic information processing (21 proteins), carbohydrate metabolism (11 proteins) and stress response (7 proteins). Somatic embryos especially displayed many stress related proteins, few enzymes involved in storage compound synthesis and an exceptional high abundance of endopeptidase inhibitors. Phosphoenolpyruvate carboxylase, which was accumulated more than 3-fold higher in zygotic embryos, represents a prominent enzyme in the storage compound metabolism in cacao seeds. Implications on the improvement of somatic embryogenesis in cacao are discussed.
AB - Somatic embryogenesis can efficiently foster the propagation of Theobroma cacao, but the poor quality of resulted plantlet hinders the use of this technique in the commercial scale. The current study has been initiated to systematically compare the physiological mechanisms underlying somatic and zygotic embryogenesis in T. cacao on the proteome level. About 1000 protein spots per fraction could be separated by two-dimensional isoelectric focusing/SDS PAGE. More than 50 of the protein spots clearly differed in abundance between zygotic and somatic embryos: 33 proteins spots were at least 3-fold higher in abundance in zygotic embryos and 20 in somatic embryos. Analyses of these protein spots differing in volume by mass spectrometry resulted in the identification of 68 distinct proteins. Many of the identified proteins are involved in genetic information processing (21 proteins), carbohydrate metabolism (11 proteins) and stress response (7 proteins). Somatic embryos especially displayed many stress related proteins, few enzymes involved in storage compound synthesis and an exceptional high abundance of endopeptidase inhibitors. Phosphoenolpyruvate carboxylase, which was accumulated more than 3-fold higher in zygotic embryos, represents a prominent enzyme in the storage compound metabolism in cacao seeds. Implications on the improvement of somatic embryogenesis in cacao are discussed.
KW - Embryogenesis
KW - Metabolic pathways
KW - Proteomics
KW - Theobroma cacao
UR - http://www.scopus.com/inward/record.url?scp=84871774170&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2012.11.007
DO - 10.1016/j.jprot.2012.11.007
M3 - Article
C2 - 23178419
AN - SCOPUS:84871774170
VL - 78
SP - 123
EP - 133
JO - Journal of proteomics
JF - Journal of proteomics
SN - 1874-3919
ER -