Details
| Original language | English |
|---|---|
| Pages (from-to) | 1111-1120 |
| Number of pages | 10 |
| Journal | Journal of cellular physiology |
| Volume | 238 |
| Issue number | 5 |
| Publication status | Published - 16 May 2023 |
Abstract
In vitro cultivation conditions play a crucial role in cell physiology and the cellular response to external stimuli. Oxygen concentrations represent an essential microenvironmental factor influencing cell physiology and behaviour both in vivo and in vitro. Therefore, new approaches are urgently needed to monitor and control oxygen concentrations in 2D and 3D cultures, as well as cell reactions to these concentrations. In this work, we modified two types of human endothelial cells–human microvascular (huMECs) and umbilical vein endothelial cells (huVECs) with genetically encoded hypoxia biosensors and monitored cell reactions in 2D to different oxygen concentrations. Moreover, we fabricated 3D cell spheroids of different cell numbers and sizes to reveal the onset of hypoxia in huVECs and huMECs. We could demonstrate a quantitative sensor response of two cell types to reduced oxygen supply in 2D and reveal different thresholds for hypoxic response. In 3D cell spheroids we could estimate critical construct sizes for the appearance of a hypoxic core. This work for the first time directly demonstrates different hypoxic signatures for huVECs and huMECs in 2D and 3D cell culture systems.
Keywords
- 3D cell culture, cell spheroids, endothelial cells, huMECs, huVECs, hypoxia
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of cellular physiology, Vol. 238, No. 5, 16.05.2023, p. 1111-1120.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Comparative analysis of hypoxic response of human microvascular and umbilical vein endothelial cells in 2D and 3D cell culture systems
AU - Dienemann, Sandra
AU - Schmidt, Vanessa
AU - Fleischhammer, Tabea
AU - Mueller, Julia H.
AU - Lavrentieva, Antonina
N1 - Funding Information: We would like to thank Vsevolod V. Belousov and Ekaterina Potekhina for providing sensor plasmids. We also thank Christopher Bartram, Marline Kirsch and Guillem Vernet Armengol for their help with data analysis. Open Access funding enabled and organized by Projekt DEAL. This study was funded by the German Research Foundation, DFG Project 398007461 488 ‘Biomolecular Sensor Platform for Elucidating Hypoxic Signatures in 2D and 3D in vitro culture Systems’.
PY - 2023/5/16
Y1 - 2023/5/16
N2 - In vitro cultivation conditions play a crucial role in cell physiology and the cellular response to external stimuli. Oxygen concentrations represent an essential microenvironmental factor influencing cell physiology and behaviour both in vivo and in vitro. Therefore, new approaches are urgently needed to monitor and control oxygen concentrations in 2D and 3D cultures, as well as cell reactions to these concentrations. In this work, we modified two types of human endothelial cells–human microvascular (huMECs) and umbilical vein endothelial cells (huVECs) with genetically encoded hypoxia biosensors and monitored cell reactions in 2D to different oxygen concentrations. Moreover, we fabricated 3D cell spheroids of different cell numbers and sizes to reveal the onset of hypoxia in huVECs and huMECs. We could demonstrate a quantitative sensor response of two cell types to reduced oxygen supply in 2D and reveal different thresholds for hypoxic response. In 3D cell spheroids we could estimate critical construct sizes for the appearance of a hypoxic core. This work for the first time directly demonstrates different hypoxic signatures for huVECs and huMECs in 2D and 3D cell culture systems.
AB - In vitro cultivation conditions play a crucial role in cell physiology and the cellular response to external stimuli. Oxygen concentrations represent an essential microenvironmental factor influencing cell physiology and behaviour both in vivo and in vitro. Therefore, new approaches are urgently needed to monitor and control oxygen concentrations in 2D and 3D cultures, as well as cell reactions to these concentrations. In this work, we modified two types of human endothelial cells–human microvascular (huMECs) and umbilical vein endothelial cells (huVECs) with genetically encoded hypoxia biosensors and monitored cell reactions in 2D to different oxygen concentrations. Moreover, we fabricated 3D cell spheroids of different cell numbers and sizes to reveal the onset of hypoxia in huVECs and huMECs. We could demonstrate a quantitative sensor response of two cell types to reduced oxygen supply in 2D and reveal different thresholds for hypoxic response. In 3D cell spheroids we could estimate critical construct sizes for the appearance of a hypoxic core. This work for the first time directly demonstrates different hypoxic signatures for huVECs and huMECs in 2D and 3D cell culture systems.
KW - 3D cell culture
KW - cell spheroids
KW - endothelial cells
KW - huMECs
KW - huVECs
KW - hypoxia
UR - http://www.scopus.com/inward/record.url?scp=85150929672&partnerID=8YFLogxK
U2 - 10.1002/jcp.31002
DO - 10.1002/jcp.31002
M3 - Article
C2 - 36947660
AN - SCOPUS:85150929672
VL - 238
SP - 1111
EP - 1120
JO - Journal of cellular physiology
JF - Journal of cellular physiology
SN - 0021-9541
IS - 5
ER -