Details
| Original language | English |
|---|---|
| Title of host publication | Difference Gel Electrophoresis (DIGE) |
| Subtitle of host publication | Methods and Protocols |
| Pages | 145-154 |
| Number of pages | 10 |
| Publication status | Published - 12 Jan 2012 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 854 |
| ISSN (Print) | 1064-3745 |
Abstract
Classically, DIGE is carried out on the basis of two-dimensional (2D) IEF/SDS PAGE. This allows comparative analyses of large protein sets. However, 2D IEF/SDS PAGE only poorly resolves hydrophobic proteins and is not compatible with native protein characterizations. Blue native PAGE represents a powerful alternative. Combined with CyDye labeling, blue native DIGE offers several useful applications like quantitative comparison of protein complexes of related protein fractions. Here we present a protocol for fluorophore labeling of native protein fractions for separation by blue native PAGE.
Keywords
- Blue native PAGE, DIGE labeling, Membrane proteins, Mitochondria, Protein complexes
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
Cite this
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Difference Gel Electrophoresis (DIGE): Methods and Protocols. 2012. p. 145-154 (Methods in Molecular Biology; Vol. 854).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Comparative Analyses of Protein Complexes by Blue Native DIGE
AU - Peters, Katrin
AU - Braun, Hans Peter
PY - 2012/1/12
Y1 - 2012/1/12
N2 - Classically, DIGE is carried out on the basis of two-dimensional (2D) IEF/SDS PAGE. This allows comparative analyses of large protein sets. However, 2D IEF/SDS PAGE only poorly resolves hydrophobic proteins and is not compatible with native protein characterizations. Blue native PAGE represents a powerful alternative. Combined with CyDye labeling, blue native DIGE offers several useful applications like quantitative comparison of protein complexes of related protein fractions. Here we present a protocol for fluorophore labeling of native protein fractions for separation by blue native PAGE.
AB - Classically, DIGE is carried out on the basis of two-dimensional (2D) IEF/SDS PAGE. This allows comparative analyses of large protein sets. However, 2D IEF/SDS PAGE only poorly resolves hydrophobic proteins and is not compatible with native protein characterizations. Blue native PAGE represents a powerful alternative. Combined with CyDye labeling, blue native DIGE offers several useful applications like quantitative comparison of protein complexes of related protein fractions. Here we present a protocol for fluorophore labeling of native protein fractions for separation by blue native PAGE.
KW - Blue native PAGE
KW - DIGE labeling
KW - Membrane proteins
KW - Mitochondria
KW - Protein complexes
UR - http://www.scopus.com/inward/record.url?scp=84858131909&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-573-2_10
DO - 10.1007/978-1-61779-573-2_10
M3 - Contribution to book/anthology
C2 - 22311758
AN - SCOPUS:84858131909
SN - 9781617795725
T3 - Methods in Molecular Biology
SP - 145
EP - 154
BT - Difference Gel Electrophoresis (DIGE)
ER -