Details
| Original language | English |
|---|---|
| Pages (from-to) | 1092-1108 |
| Number of pages | 17 |
| Journal | PHYTOCHEMISTRY |
| Volume | 72 |
| Issue number | 10 |
| Publication status | Published - Jul 2011 |
| Externally published | Yes |
Abstract
Mitochondria undertake respiration in plant cells, but through metabolic plasticity utilize differ proportions of substrates and deliver different proportions of products to cellular metabolic and biosynthetic pathways. In Arabidopsis the mitochondrial proteome from shoots and cell culture have been reported, but there has been little information on mitochondria in roots. We compare the root mitochondrial proteome with mitochondria isolated from photosynthetic shoots to define the role of protein abundance in these differences. The major differences observed were in the abundance and/or activities of enzymes in the TCA cycle and the mitochondrial enzymes involved in photorespiration. Metabolic pathways linked to TCA cycle and photorespiration were also altered, namely cysteine, formate and one-carbon metabolism, as well as amino acid metabolism focused on 2-oxoglutarate generation. Comparisons to microarray analysis of these same tissues showed a positive correlation between mRNA and mitochondrial protein abundance, but still ample evidence for the role of post-transcriptional processes in defining mitochondrial composition. Broader comparisons of transcript abundances for mitochondrial components across Arabidopsis tissues provided additional evidence for specialization of plant mitochondria, and clustering of these data in functional groups showed the constitutive vs variably expressed components of plant mitochondria.
Keywords
- Arabidopsis thaliana, Mitochondria, Photorespiration, Proteomics, TCA cycle
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Agricultural and Biological Sciences(all)
- Plant Science
- Agricultural and Biological Sciences(all)
- Horticulture
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: PHYTOCHEMISTRY, Vol. 72, No. 10, 07.2011, p. 1092-1108.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Combining proteomics of root and shoot mitochondria and transcript analysis to define constitutive and variable components in plant mitochondria
AU - Lee, Chun Pong
AU - Eubel, Holger
AU - O'Toole, Nicholas
AU - Millar, A. Harvey
N1 - Funding information: This work was supported by grants from the Australian Research Council (ARC) through the Centres of Excellence Program ( CE0561495 ) and by the WA State Government support to the Centre for Computational Systems Biology. C.P.L. is supported by an Australian Postgraduate Award, H.E. by an ARC Australian Post-Doctoral Fellowship and A.H.M. by an ARC Australian Professorial Fellowship.
PY - 2011/7
Y1 - 2011/7
N2 - Mitochondria undertake respiration in plant cells, but through metabolic plasticity utilize differ proportions of substrates and deliver different proportions of products to cellular metabolic and biosynthetic pathways. In Arabidopsis the mitochondrial proteome from shoots and cell culture have been reported, but there has been little information on mitochondria in roots. We compare the root mitochondrial proteome with mitochondria isolated from photosynthetic shoots to define the role of protein abundance in these differences. The major differences observed were in the abundance and/or activities of enzymes in the TCA cycle and the mitochondrial enzymes involved in photorespiration. Metabolic pathways linked to TCA cycle and photorespiration were also altered, namely cysteine, formate and one-carbon metabolism, as well as amino acid metabolism focused on 2-oxoglutarate generation. Comparisons to microarray analysis of these same tissues showed a positive correlation between mRNA and mitochondrial protein abundance, but still ample evidence for the role of post-transcriptional processes in defining mitochondrial composition. Broader comparisons of transcript abundances for mitochondrial components across Arabidopsis tissues provided additional evidence for specialization of plant mitochondria, and clustering of these data in functional groups showed the constitutive vs variably expressed components of plant mitochondria.
AB - Mitochondria undertake respiration in plant cells, but through metabolic plasticity utilize differ proportions of substrates and deliver different proportions of products to cellular metabolic and biosynthetic pathways. In Arabidopsis the mitochondrial proteome from shoots and cell culture have been reported, but there has been little information on mitochondria in roots. We compare the root mitochondrial proteome with mitochondria isolated from photosynthetic shoots to define the role of protein abundance in these differences. The major differences observed were in the abundance and/or activities of enzymes in the TCA cycle and the mitochondrial enzymes involved in photorespiration. Metabolic pathways linked to TCA cycle and photorespiration were also altered, namely cysteine, formate and one-carbon metabolism, as well as amino acid metabolism focused on 2-oxoglutarate generation. Comparisons to microarray analysis of these same tissues showed a positive correlation between mRNA and mitochondrial protein abundance, but still ample evidence for the role of post-transcriptional processes in defining mitochondrial composition. Broader comparisons of transcript abundances for mitochondrial components across Arabidopsis tissues provided additional evidence for specialization of plant mitochondria, and clustering of these data in functional groups showed the constitutive vs variably expressed components of plant mitochondria.
KW - Arabidopsis thaliana
KW - Mitochondria
KW - Photorespiration
KW - Proteomics
KW - TCA cycle
UR - http://www.scopus.com/inward/record.url?scp=79958172874&partnerID=8YFLogxK
U2 - 10.1016/j.phytochem.2010.12.004
DO - 10.1016/j.phytochem.2010.12.004
M3 - Article
AN - SCOPUS:79958172874
VL - 72
SP - 1092
EP - 1108
JO - PHYTOCHEMISTRY
JF - PHYTOCHEMISTRY
SN - 0031-9422
IS - 10
ER -