Details
| Original language | English |
|---|---|
| Pages (from-to) | 1447-1459 |
| Number of pages | 13 |
| Journal | Nanoscale Advances |
| Volume | 6 |
| Issue number | 5 |
| Publication status | Published - 8 Feb 2024 |
Abstract
Early detection of specific oral bacterial species would enable timely treatment and prevention of certain oral diseases. In this work, we investigated the sensitivity and specificity of functionalized gold nanoparticles for plasmonic sensing of oral bacteria. This approach is based on the aggregation of positively charged gold nanoparticles on the negatively charged bacteria surface and the corresponding localized surface plasmon resonance (LSPR) shift. Gold nanoparticles were synthesized in different sizes, shapes and functionalization. A biosensor array was developed consisting of spherical- and anisotropic-shaped (1-hexadecyl) trimethylammonium bromide (CTAB) and spherical mercaptoethylamine (MEA) gold nanoparticles. It was used to detect four oral bacterial species (Aggregatibacter actinomycetemcomitans, Actinomyces naeslundii, Porphyromonas gingivalis and Streptococcus oralis). The plasmonic response was measured and analysed using RGB and UV-vis absorbance values. Both methods successfully detected the individual bacterial species based on their unique responses to the biosensor array. We present an in-depth study relating the bacteria zeta potential and AuNP aggregation to plasmonic response. The sensitivity depends on multiple parameters, such as bacterial species and concentration as well as gold nanoparticle shape, concentration and functionalization.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Chemistry(all)
- Materials Science(all)
- Engineering(all)
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In: Nanoscale Advances, Vol. 6, No. 5, 08.02.2024, p. 1447-1459 .
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Colorimetric detection of oral bacteria using functionalized gold nanoparticles as a plasmonic biosensor array
AU - Wenck, Christina
AU - Leopoldt, Dorthe
AU - Habib, Mosaieb
AU - Hegermann, Jan
AU - Stiesch, Meike
AU - Doll-Nikutta, Katharina
AU - Heisterkamp, Alexander
AU - Torres-Mapa, Maria Leilani
N1 - Funding Information: The work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under the Collaborative Research Center SFB/TRR-298-SIIRI—Project ID 426335750 (MS, K. D-N and AH) and partially under Germany's Excellence Strategy (DFG Cluster of Excellence) EXC 2177/1-Hearing4All—Project ID 390895286 (AH). MLT-M acknowledges funding from Caroline Herschel Fellowship, Leibniz University Hannover.
PY - 2024/2/8
Y1 - 2024/2/8
N2 - Early detection of specific oral bacterial species would enable timely treatment and prevention of certain oral diseases. In this work, we investigated the sensitivity and specificity of functionalized gold nanoparticles for plasmonic sensing of oral bacteria. This approach is based on the aggregation of positively charged gold nanoparticles on the negatively charged bacteria surface and the corresponding localized surface plasmon resonance (LSPR) shift. Gold nanoparticles were synthesized in different sizes, shapes and functionalization. A biosensor array was developed consisting of spherical- and anisotropic-shaped (1-hexadecyl) trimethylammonium bromide (CTAB) and spherical mercaptoethylamine (MEA) gold nanoparticles. It was used to detect four oral bacterial species (Aggregatibacter actinomycetemcomitans, Actinomyces naeslundii, Porphyromonas gingivalis and Streptococcus oralis). The plasmonic response was measured and analysed using RGB and UV-vis absorbance values. Both methods successfully detected the individual bacterial species based on their unique responses to the biosensor array. We present an in-depth study relating the bacteria zeta potential and AuNP aggregation to plasmonic response. The sensitivity depends on multiple parameters, such as bacterial species and concentration as well as gold nanoparticle shape, concentration and functionalization.
AB - Early detection of specific oral bacterial species would enable timely treatment and prevention of certain oral diseases. In this work, we investigated the sensitivity and specificity of functionalized gold nanoparticles for plasmonic sensing of oral bacteria. This approach is based on the aggregation of positively charged gold nanoparticles on the negatively charged bacteria surface and the corresponding localized surface plasmon resonance (LSPR) shift. Gold nanoparticles were synthesized in different sizes, shapes and functionalization. A biosensor array was developed consisting of spherical- and anisotropic-shaped (1-hexadecyl) trimethylammonium bromide (CTAB) and spherical mercaptoethylamine (MEA) gold nanoparticles. It was used to detect four oral bacterial species (Aggregatibacter actinomycetemcomitans, Actinomyces naeslundii, Porphyromonas gingivalis and Streptococcus oralis). The plasmonic response was measured and analysed using RGB and UV-vis absorbance values. Both methods successfully detected the individual bacterial species based on their unique responses to the biosensor array. We present an in-depth study relating the bacteria zeta potential and AuNP aggregation to plasmonic response. The sensitivity depends on multiple parameters, such as bacterial species and concentration as well as gold nanoparticle shape, concentration and functionalization.
UR - http://www.scopus.com/inward/record.url?scp=85184582832&partnerID=8YFLogxK
U2 - 10.1039/d3na00477e
DO - 10.1039/d3na00477e
M3 - Article
AN - SCOPUS:85184582832
VL - 6
SP - 1447
EP - 1459
JO - Nanoscale Advances
JF - Nanoscale Advances
IS - 5
ER -