Details
| Original language | English |
|---|---|
| Pages (from-to) | 37-43 |
| Number of pages | 7 |
| Journal | International Journal of Bioprinting |
| Volume | 2 |
| Issue number | 2 |
| Publication status | Published - 22 Jun 2016 |
Abstract
Laser Induced Forward Transfer (LIFT) bioprinting is one of a group of techniques that have been largely applied for printing mammalian cells so far. Bioprinting allows precise placement of viable cells in a defined matrix with the aim of directed three-dimensional development of tissues. In this study, laser bioprinting is used to precisely place eukaryotic microorganisms in specific patterns that allow growth and microscopic observation of the organisms' micro-colonies. Saccharomyces cerevisiae var. bayanus and Chlorella vulgaris are used as model organisms for this purpose. Growth and development of the micro-colonies are studied via confocal microscopy and the colonies' growth rates are determined by image analysis. The developed protocols for printing of microorganisms and growth-rate deter-mination of the micro-colonies are very promising for future studies of colony growth and development.
Keywords
- Bioprinting, Chlorella vulgaris, Growth rates of micro-colonies, Laser-induced forward transfer, Printing of microorganisms, Saccharomyces cerevisiae
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Materials Science(all)
- Materials Science (miscellaneous)
- Engineering(all)
- Industrial and Manufacturing Engineering
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In: International Journal of Bioprinting, Vol. 2, No. 2, 22.06.2016, p. 37-43.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Colony development of laser printed eukaryotic (yeast and microalga) microorganisms in co-culture
AU - Taidi, Behnam
AU - Lebernede, Guillaume
AU - Koch, Lothar
AU - Perre, Patrick
AU - Chichkov, Boris
PY - 2016/6/22
Y1 - 2016/6/22
N2 - Laser Induced Forward Transfer (LIFT) bioprinting is one of a group of techniques that have been largely applied for printing mammalian cells so far. Bioprinting allows precise placement of viable cells in a defined matrix with the aim of directed three-dimensional development of tissues. In this study, laser bioprinting is used to precisely place eukaryotic microorganisms in specific patterns that allow growth and microscopic observation of the organisms' micro-colonies. Saccharomyces cerevisiae var. bayanus and Chlorella vulgaris are used as model organisms for this purpose. Growth and development of the micro-colonies are studied via confocal microscopy and the colonies' growth rates are determined by image analysis. The developed protocols for printing of microorganisms and growth-rate deter-mination of the micro-colonies are very promising for future studies of colony growth and development.
AB - Laser Induced Forward Transfer (LIFT) bioprinting is one of a group of techniques that have been largely applied for printing mammalian cells so far. Bioprinting allows precise placement of viable cells in a defined matrix with the aim of directed three-dimensional development of tissues. In this study, laser bioprinting is used to precisely place eukaryotic microorganisms in specific patterns that allow growth and microscopic observation of the organisms' micro-colonies. Saccharomyces cerevisiae var. bayanus and Chlorella vulgaris are used as model organisms for this purpose. Growth and development of the micro-colonies are studied via confocal microscopy and the colonies' growth rates are determined by image analysis. The developed protocols for printing of microorganisms and growth-rate deter-mination of the micro-colonies are very promising for future studies of colony growth and development.
KW - Bioprinting
KW - Chlorella vulgaris
KW - Growth rates of micro-colonies
KW - Laser-induced forward transfer
KW - Printing of microorganisms
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=85045298288&partnerID=8YFLogxK
U2 - 10.18063/IJB.2016.02.001
DO - 10.18063/IJB.2016.02.001
M3 - Article
AN - SCOPUS:85045298288
VL - 2
SP - 37
EP - 43
JO - International Journal of Bioprinting
JF - International Journal of Bioprinting
IS - 2
ER -