Cold generation of smoke flavour by the first phenolic acid decarboxylase from a filamentous ascomycete: Isaria farinosa

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Authors

  • Diana Linke
  • Stephanie Riemer
  • Silke Schimanski
  • Annabel Nieter
  • Ulrich Krings
  • Ralf G. Berger

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Details

Original languageEnglish
Pages (from-to)763-774
Number of pages12
JournalFungal biology
Volume121
Issue number9
Publication statusPublished - 3 Jun 2017

Abstract

A decarboxylase (IfPAD) from the ascomycete Isaria farinosa converted ferulic acid to 4-vinylguaiacol (4-VG), a volatile which imparts the distinct “smoke flavor” of pyrolized wood. The activity was enhanced by adding (E)-ferulic acid to the culture medium and peaked with 3.6 U g−1 mycelium (1 μmol 4-VG min−1). The coding sequence of 543 bp was translated into a 25 kDa protein with a homology of 91 % to putative phenolic acid decarboxylases of its teleomorph, Cordyceps militaris, and Beauveria bassiana, the anamorph of Cordyceps bassiana. Cold shock expression in Escherichia coli yielded 411 U g−1 wet mass. Substrate conversion required a hydroxyl substituent para to a trans-unsaturated C3-side chain of the aromatic ring. Km and kcat/Km values were determined to 0.3 mM and 78.4 mM−1s−1 for p-coumaric acid and 1.9 mM and 45.1 mM−1s−1 for (E)-ferulic acid, respectively. The native enzyme and its recombinant counterpart showed pH-optima at pH 6.0 and pH 5.5, and low temperature optima of 19 °C and 14 °C, respectively. IfPAD produced 4-VG from destarched wheat bran and sugar beet fiber, confirming activity on complex plant biomass. This is the first report on the biochemical characterization of a phenolic acid decarboxylase from a filamentous ascomycete.

Keywords

    (E)-ferulic acid, 4-Vinylguaiacol, Biotransformation, Complex plant biomass, Filamentous fungus, PAD family

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Sustainable Development Goals

Cite this

Cold generation of smoke flavour by the first phenolic acid decarboxylase from a filamentous ascomycete: Isaria farinosa. / Linke, Diana; Riemer, Stephanie; Schimanski, Silke et al.
In: Fungal biology, Vol. 121, No. 9, 03.06.2017, p. 763-774.

Research output: Contribution to journalArticleResearchpeer review

Linke D, Riemer S, Schimanski S, Nieter A, Krings U, Berger RG. Cold generation of smoke flavour by the first phenolic acid decarboxylase from a filamentous ascomycete: Isaria farinosa. Fungal biology. 2017 Jun 3;121(9):763-774. doi: 10.1016/j.funbio.2017.05.006
Linke, Diana ; Riemer, Stephanie ; Schimanski, Silke et al. / Cold generation of smoke flavour by the first phenolic acid decarboxylase from a filamentous ascomycete : Isaria farinosa. In: Fungal biology. 2017 ; Vol. 121, No. 9. pp. 763-774.
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abstract = "A decarboxylase (IfPAD) from the ascomycete Isaria farinosa converted ferulic acid to 4-vinylguaiacol (4-VG), a volatile which imparts the distinct “smoke flavor” of pyrolized wood. The activity was enhanced by adding (E)-ferulic acid to the culture medium and peaked with 3.6 U g−1 mycelium (1 μmol 4-VG min−1). The coding sequence of 543 bp was translated into a 25 kDa protein with a homology of 91 % to putative phenolic acid decarboxylases of its teleomorph, Cordyceps militaris, and Beauveria bassiana, the anamorph of Cordyceps bassiana. Cold shock expression in Escherichia coli yielded 411 U g−1 wet mass. Substrate conversion required a hydroxyl substituent para to a trans-unsaturated C3-side chain of the aromatic ring. Km and kcat/Km values were determined to 0.3 mM and 78.4 mM−1s−1 for p-coumaric acid and 1.9 mM and 45.1 mM−1s−1 for (E)-ferulic acid, respectively. The native enzyme and its recombinant counterpart showed pH-optima at pH 6.0 and pH 5.5, and low temperature optima of 19 °C and 14 °C, respectively. IfPAD produced 4-VG from destarched wheat bran and sugar beet fiber, confirming activity on complex plant biomass. This is the first report on the biochemical characterization of a phenolic acid decarboxylase from a filamentous ascomycete.",
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T2 - Isaria farinosa

AU - Linke, Diana

AU - Riemer, Stephanie

AU - Schimanski, Silke

AU - Nieter, Annabel

AU - Krings, Ulrich

AU - Berger, Ralf G.

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