Details
| Original language | English |
|---|---|
| Pages (from-to) | 234-244 |
| Number of pages | 11 |
| Journal | Macromolecular bioscience |
| Volume | 11 |
| Issue number | 2 |
| Early online date | 15 Nov 2010 |
| Publication status | Published - 1 Feb 2011 |
Abstract
A fast and simple approach for immobilization using copolymers as interlayers is reported. The synthesized copolymers form stable self-assembled layers on implant materials like, e.g., titanium in a simple coating/drying/washing sequence and have functional groups which can bind proteins from an aqueous solution. The copolymer films have been characterized via ellipsometry and contact angle measurements and were tested for biocompatibility. An immunoassay was used to determine the amount of BMP2 and demonstrated an approximately 10-fold increase as compared to previously used self-assembled monolayers.1 A BMP2-responsive cell line with luciferase detection was used to determine the biological activity of the bound signaling protein. Copolymers were prepared that attach easily to titanium/titanium oxide surfaces and bind proteins without further modification. The structure of the partially crosslinked copolymer allows binding of the protein not only at the surface but also within the film. The growth factor BMP2 can be bound to the copolymer and remains biologically active.
Keywords
- Biological application of polymers, Copolymerization, Implant, Modification, Proteins
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Materials Science(all)
- Biomaterials
- Materials Science(all)
- Polymers and Plastics
- Materials Science(all)
- Materials Chemistry
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In: Macromolecular bioscience, Vol. 11, No. 2, 01.02.2011, p. 234-244.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Coating of Titanium Implant Materials with Thin Polymeric Films for Binding the Signaling Protein BMP2
AU - Lorenz, Corinna
AU - Hoffmann, Andrea
AU - Gross, Gerhard
AU - Windhagen, Henning
AU - Dellinger, Philip
AU - Möhwald, Kai
AU - Dempwolf, Wibke
AU - Menzel, Henning
PY - 2011/2/1
Y1 - 2011/2/1
N2 - A fast and simple approach for immobilization using copolymers as interlayers is reported. The synthesized copolymers form stable self-assembled layers on implant materials like, e.g., titanium in a simple coating/drying/washing sequence and have functional groups which can bind proteins from an aqueous solution. The copolymer films have been characterized via ellipsometry and contact angle measurements and were tested for biocompatibility. An immunoassay was used to determine the amount of BMP2 and demonstrated an approximately 10-fold increase as compared to previously used self-assembled monolayers.1 A BMP2-responsive cell line with luciferase detection was used to determine the biological activity of the bound signaling protein. Copolymers were prepared that attach easily to titanium/titanium oxide surfaces and bind proteins without further modification. The structure of the partially crosslinked copolymer allows binding of the protein not only at the surface but also within the film. The growth factor BMP2 can be bound to the copolymer and remains biologically active.
AB - A fast and simple approach for immobilization using copolymers as interlayers is reported. The synthesized copolymers form stable self-assembled layers on implant materials like, e.g., titanium in a simple coating/drying/washing sequence and have functional groups which can bind proteins from an aqueous solution. The copolymer films have been characterized via ellipsometry and contact angle measurements and were tested for biocompatibility. An immunoassay was used to determine the amount of BMP2 and demonstrated an approximately 10-fold increase as compared to previously used self-assembled monolayers.1 A BMP2-responsive cell line with luciferase detection was used to determine the biological activity of the bound signaling protein. Copolymers were prepared that attach easily to titanium/titanium oxide surfaces and bind proteins without further modification. The structure of the partially crosslinked copolymer allows binding of the protein not only at the surface but also within the film. The growth factor BMP2 can be bound to the copolymer and remains biologically active.
KW - Biological application of polymers
KW - Copolymerization
KW - Implant
KW - Modification
KW - Proteins
UR - http://www.scopus.com/inward/record.url?scp=79251543952&partnerID=8YFLogxK
U2 - 10.1002/mabi.201000342
DO - 10.1002/mabi.201000342
M3 - Article
C2 - 21080383
AN - SCOPUS:79251543952
VL - 11
SP - 234
EP - 244
JO - Macromolecular bioscience
JF - Macromolecular bioscience
SN - 1616-5187
IS - 2
ER -