Details
| Original language | English |
|---|---|
| Pages (from-to) | 863-867 |
| Number of pages | 5 |
| Journal | Plant cell reports |
| Volume | 18 |
| Issue number | 10 |
| Publication status | Published - Jun 1999 |
| Externally published | Yes |
Abstract
CO2 accumulation in different culture systems containing embryogenic cell suspension cultures of cyclamen (Cyclamen persicum Mill.) was analyzed. In bioreactors equipped with a bubble-free or a bubble aeration system, CO2 mole fractions in the gas phase of more than 10% were determined whereas in Erlenmeyer flasks, CO2 mole fractions were below 2%. CO2 accumulation in bioreactors was severely growth inhibiting in comparison to the flasks. By removing CO2 in the aeration gas of a bubble-free aerated bioreactor, cell growth comparable to that in flasks was achieved. The regeneration ability of cell suspensions after being cultured in bioreactors with CO2 accumulation was better than those after culture in bioreactors without CO2 accumulation or in flasks.
Keywords
- Bioreactor, Carbon dioxide, Cell suspension culture, Cyclamen persicum Mill., Somatic embryogenesis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant cell reports, Vol. 18, No. 10, 06.1999, p. 863-867.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - CO2 accumulation in bioreactor suspension cultures of Cyclamen persicum Mill. and its effect on cell growth and regeneration of somatic embryos
AU - Hohe, A.
AU - Winkelmann, T.
AU - Schwenkel, H. G.
PY - 1999/6
Y1 - 1999/6
N2 - CO2 accumulation in different culture systems containing embryogenic cell suspension cultures of cyclamen (Cyclamen persicum Mill.) was analyzed. In bioreactors equipped with a bubble-free or a bubble aeration system, CO2 mole fractions in the gas phase of more than 10% were determined whereas in Erlenmeyer flasks, CO2 mole fractions were below 2%. CO2 accumulation in bioreactors was severely growth inhibiting in comparison to the flasks. By removing CO2 in the aeration gas of a bubble-free aerated bioreactor, cell growth comparable to that in flasks was achieved. The regeneration ability of cell suspensions after being cultured in bioreactors with CO2 accumulation was better than those after culture in bioreactors without CO2 accumulation or in flasks.
AB - CO2 accumulation in different culture systems containing embryogenic cell suspension cultures of cyclamen (Cyclamen persicum Mill.) was analyzed. In bioreactors equipped with a bubble-free or a bubble aeration system, CO2 mole fractions in the gas phase of more than 10% were determined whereas in Erlenmeyer flasks, CO2 mole fractions were below 2%. CO2 accumulation in bioreactors was severely growth inhibiting in comparison to the flasks. By removing CO2 in the aeration gas of a bubble-free aerated bioreactor, cell growth comparable to that in flasks was achieved. The regeneration ability of cell suspensions after being cultured in bioreactors with CO2 accumulation was better than those after culture in bioreactors without CO2 accumulation or in flasks.
KW - Bioreactor
KW - Carbon dioxide
KW - Cell suspension culture
KW - Cyclamen persicum Mill.
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=0032979170&partnerID=8YFLogxK
U2 - 10.1007/s002990050675
DO - 10.1007/s002990050675
M3 - Article
AN - SCOPUS:0032979170
VL - 18
SP - 863
EP - 867
JO - Plant cell reports
JF - Plant cell reports
SN - 0721-7714
IS - 10
ER -