Details
| Original language | English |
|---|---|
| Pages (from-to) | 6271-6278 |
| Number of pages | 8 |
| Journal | Journal of Bacteriology |
| Volume | 179 |
| Issue number | 20 |
| Publication status | Published - Oct 1997 |
| Externally published | Yes |
Abstract
A 65-kb legion of DNA from Streptomyces viridochromogenes Tu57, containing genes encoding proteins involved in the biosynthesis of avilamycins, was isolated. The DNA sequence of a 6.4-kb fragment from this region revealed four open reading frames (ORF1 to ORF4), three of which are fully contained within the sequenced fragment. The deduced amino acid sequence of AviM, encoded by ORF2, shows 37% identity to a 6-methylsalicylic acid synthase from Penicillium patulum. Cultures of S. lividans TK24 and S. coelicolor CH999 containing plasmids with ORF2 on a 5.5-kb PstI fragment were able to produce orsellinic acid, an unreduced version of 6-methylsalicylic acid. The amino acid sequence encoded by ORF3 (AviD) is 62% identical to that of StrD, a dTDP-glucose synthase from S. griseus. The deduced amino acid sequence of AviE, encoded by ORF4, shows 55% identity to a dTDP-glucose dehydratase (StrE) from S. griseus. Gene insertional inactivation experiments of aviE abolished avilamycin production, indicating the involvement of aviE in the biosynthesis of avilamycins.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: Journal of Bacteriology, Vol. 179, No. 20, 10.1997, p. 6271-6278.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Cloning of an avilamycin biosynthetic gene cluster from Streptomyces viridochromogenes Tu57
AU - Gaisser, Sibylle
AU - Trefzer, Axel
AU - Stockert, Sigrid
AU - Kirschning, Andreas
AU - Bechthold, Andreas
PY - 1997/10
Y1 - 1997/10
N2 - A 65-kb legion of DNA from Streptomyces viridochromogenes Tu57, containing genes encoding proteins involved in the biosynthesis of avilamycins, was isolated. The DNA sequence of a 6.4-kb fragment from this region revealed four open reading frames (ORF1 to ORF4), three of which are fully contained within the sequenced fragment. The deduced amino acid sequence of AviM, encoded by ORF2, shows 37% identity to a 6-methylsalicylic acid synthase from Penicillium patulum. Cultures of S. lividans TK24 and S. coelicolor CH999 containing plasmids with ORF2 on a 5.5-kb PstI fragment were able to produce orsellinic acid, an unreduced version of 6-methylsalicylic acid. The amino acid sequence encoded by ORF3 (AviD) is 62% identical to that of StrD, a dTDP-glucose synthase from S. griseus. The deduced amino acid sequence of AviE, encoded by ORF4, shows 55% identity to a dTDP-glucose dehydratase (StrE) from S. griseus. Gene insertional inactivation experiments of aviE abolished avilamycin production, indicating the involvement of aviE in the biosynthesis of avilamycins.
AB - A 65-kb legion of DNA from Streptomyces viridochromogenes Tu57, containing genes encoding proteins involved in the biosynthesis of avilamycins, was isolated. The DNA sequence of a 6.4-kb fragment from this region revealed four open reading frames (ORF1 to ORF4), three of which are fully contained within the sequenced fragment. The deduced amino acid sequence of AviM, encoded by ORF2, shows 37% identity to a 6-methylsalicylic acid synthase from Penicillium patulum. Cultures of S. lividans TK24 and S. coelicolor CH999 containing plasmids with ORF2 on a 5.5-kb PstI fragment were able to produce orsellinic acid, an unreduced version of 6-methylsalicylic acid. The amino acid sequence encoded by ORF3 (AviD) is 62% identical to that of StrD, a dTDP-glucose synthase from S. griseus. The deduced amino acid sequence of AviE, encoded by ORF4, shows 55% identity to a dTDP-glucose dehydratase (StrE) from S. griseus. Gene insertional inactivation experiments of aviE abolished avilamycin production, indicating the involvement of aviE in the biosynthesis of avilamycins.
UR - http://www.scopus.com/inward/record.url?scp=0030744904&partnerID=8YFLogxK
U2 - 10.1128/jb.179.20.6271-6278.1997
DO - 10.1128/jb.179.20.6271-6278.1997
M3 - Article
C2 - 9335272
AN - SCOPUS:0030744904
VL - 179
SP - 6271
EP - 6278
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 20
ER -